LOCUS       Exported               14694 bp ds-DNA     circular SYN 24-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 24, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2688..2958
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2991..3029
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2991..3000
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3162..3878
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(5071..5095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5145..6356
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6193..6212
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6481..7227
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7265..7838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7432..7449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7659..7678
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7897..8916
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    9382..9970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9435..9455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9853..9864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9973..9989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9974..9990)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10499..10679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10705..10722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10711..10935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10981..11409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11068..11087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11278..11299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11418..11438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11423..11752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11603..11738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11665..11684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11800..11847
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11866..12240
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12370..12491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12407..12426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12461..12480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12540..12556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12540..12556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12553..12575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12564..12580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12588..12618)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12633..12654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12771..12788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12942..13530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13022..13041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13701..14561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14324..14343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14562..14666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttc
     2641 attacaggga cagcagagat ccagtttgga ctaggatcct ttaccactcc ctatcagtga
     2701 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2761 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2821 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2881 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2941 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg
     3001 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     3061 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
     3121 ccgaactagt ccagtgtggt ggaattctgc agatatcaac catggtgagc aagggcgagg
     3181 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3241 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3301 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct
     3361 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3421 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3481 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3541 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact
     3601 ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3661 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     3721 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     3781 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3841 ccgccgggat cactctcggc atggacgagc tgtacaagag atccatggac gcagccactt
     3901 tgacatatga tactctccgg tttgctgaat ttgaagattt ccctgagacc tcagagcctg
     3961 tttggattct gggcagaaaa tacagcattt tcacagagaa ggacgaaatc ttgtctgatg
     4021 ttgcatccag actttggttt acatacagga gaaactttcc agctattggg ggaactggcc
     4081 ctacttcaga cacaggctgg ggtgcaatgc ttcggtgtgg acagatgatc tttgcccagg
     4141 ccctggtatg ccggcactta ggtcgagatt ggaggtggac tcagcggaag aggcagcctg
     4201 acagctactt taatgtcctc aatgctttcc tcgacaggaa ggacagctac tattccatcc
     4261 atcagatagc gcaaatggga gttggcgaag gcaagtctat aggccagtgg tacgggccga
     4321 acactgttgc ccaggtcctc aagaaacttg ctgtattcga cacatggagt tccttggctg
     4381 ttcacatagc aatggacaac actgtggtga tggaggaaat cagaaggtta tgcagggcca
     4441 accttccctg tgttggggcg gctgcacttc ctactgattc agagaggcac tgtaatgggt
     4501 tccctgctgg agctgaagtc accaacaggc catcggcttg gagaccacta gtgcttctca
     4561 tccctctccg cctgggactg acagacatca atgaggccta tgtggagacg ctgaagcact
     4621 gtttcatgat gccccagtcc ctgggtgtta ttggagggaa gcccaacagt gcccactact
     4681 ttattggcta tgttggggag gagctcatct atctggaccc ccacactaca cagcctgcag
     4741 tggagctgac tgacagctgc ttcatccccg acgagagctt ccactgccag caccctccct
     4801 ctaggatggg cattggagag cttgacccgt ccattgctgt ggggtttttc tgtaagacgg
     4861 aggaagactt taacgactgg tgtcagcaag tcaaaaagct atcccagctt ggaggtgccc
     4921 tgcccatgtt tgagctggtg gaacagcagc cttcccatct ggcctgccag gatgtcctga
     4981 acctgtccct agactcttct gatgtagaga gactggagag gttttttgac tctgaggatg
     5041 aagactttga aatcttatcc ctctgataaa acccagcttt cttgtacaaa gtggttgata
     5101 acatctcgag ccataaagat ggttaattaa cccacccaag atctggcctc cgcgccgggt
     5161 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     5221 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     5281 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     5341 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     5401 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     5461 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     5521 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     5581 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     5641 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     5701 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     5761 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     5821 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     5881 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     5941 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     6001 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     6061 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     6121 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     6181 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     6241 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     6301 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     6361 cttggtaccg agctcggatc tccaccccgt accggtcctg caggatttaa atggcgcgcc
     6421 gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt cgaattcacc
     6481 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     6541 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     6601 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     6661 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     6721 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     6781 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     6841 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     6901 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     6961 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     7021 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     7081 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     7141 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     7201 tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat
     7261 tccgcccctc tccccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     7321 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     7381 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     7441 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     7501 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     7561 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     7621 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     7681 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     7741 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     7801 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttaccggtac gcgtgatagc
     7861 ttgccacaac ccgtaccaaa gatggataga tccggaaagc ctgaactcac cgcgacgtct
     7921 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     7981 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     8041 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     8101 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     8161 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     8221 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     8281 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     8341 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     8401 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     8461 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     8521 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     8581 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     8641 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     8701 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     8761 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     8821 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     8881 cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc
     8941 tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga
     9001 acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg
     9061 tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca
     9121 cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc
     9181 ccgtgcctgc cgattgggtg cagatctcta gatctgtaca agtaaagcgg ccgcgactct
     9241 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9301 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     9361 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     9421 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9481 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9541 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9601 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9661 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9781 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9841 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9901 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9961 gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac
    10021 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
    10081 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
    10141 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
    10201 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
    10261 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
    10321 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
    10381 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
    10441 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
    10501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10681 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
    10741 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
    10801 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
    10861 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
    10921 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
    10981 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    11041 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    11101 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    11161 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    11221 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    11281 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    11341 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    11401 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    11461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    11521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    11581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
    11701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    11761 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
    11821 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
    11881 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
    11941 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
    12001 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
    12061 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
    12121 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
    12181 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
    12241 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    12301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    12361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    12421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    12481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    12541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    12601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    12661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    12721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    12781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    12841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    12901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    12961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    13021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    13081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    13141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    13201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    13261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    13321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    13381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    13441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    13501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    13561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    13621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    13681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    13741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    13801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    13861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    13921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    13981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    14041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    14101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    14161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    14221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    14281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    14341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    14401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    14461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    14521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    14581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    14641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
