LOCUS       pTRANS-Pho2-1/2-2-Csy4-Addgene_#161763       17682 bp    DNA     circular UNA 02-OCT-2020
DEFINITION  pTRANS T-DNA with nptII selection and 35S:Cas9 with 6x Csy4-spliced
            gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and
            MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113).
ACCESSION   urn.local...fwe-c141g9j
VERSION     urn.local...fwe-c141g9j
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            complement(291..1085)
                     /note="aadA (kanamycin resistance) gene amplified from
                     pIG121Hm; kanamycin gene amplified from pIG121Hm"
                     /product="aminoglycoside phosphotransferase"
                     /db_xref="GI:7638070; GI:7638076; GI:7638147; GI:7638151"
                     /standard_name="KanR"
     misc_feature    1510..1535
                     /note="Left border repeat from C58 T-DNA"
                     /standard_name="T-DNA border (L)"
     terminator      complement(1602..1792)
     CDS             complement(1843..2640)
                     /standard_name="neomycin phosphotransferase (kanR)"
     promoter        complement(2671..3439)
     misc_feature    3715..9625
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pMOD_A0501 - Fragment 1
                     (concatenated sequence 2)"
     misc_feature    3715..3718
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     promoter        3727..4568
     CDS             4581..5141
                     /standard_name="Csy4"
     misc_feature    5142..5150
                     /standard_name="GSG linker"
     misc_feature    5151..5207
                     /standard_name="P2A"
     CDS             5208..9347
                     /standard_name="AtCas9"
     misc_feature    5235..5237
                     /standard_name="D10"
     misc_feature    7725..7727
                     /standard_name="H840"
     misc_feature    9312..9344
                     /note="Geneious type: NLS"
                     /standard_name="SV40 NLS (with linker)"
     terminator      9356..9605
     misc_feature    9533..9552
                     /standard_name="TC 430 primer"
     misc_feature    9622..11036
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of
                     pMOD_B2103_PHOAC_B_TARGETS_120617_txt - Fragment 1
                     (concatenated sequence 3)"
     misc_feature    9622..9625
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     promoter        9633..10097
     misc_feature    10105..10124
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10125..10144
                     /note="Geneious type: misc"
                     /note="Phoac-1"
                     /standard_name="Pho2-1abc-1 (formerly Phoac-1)"
     misc_feature    10145..10220
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10221..10240
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10241..10260
                     /note="Geneious type: misc"
                     /note="Phoac-2"
                     /standard_name="Pho2-1abcd-2 (formerly Phoac-2)"
     misc_feature    10261..10336
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10337..10356
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10357..10376
                     /note="Geneious type: misc"
                     /note="Phoac-3"
                     /standard_name="Pho2-1abcd-3 (formerly Phoac-3)"
     misc_feature    10377..10452
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10453..10472
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10473..10492
                     /note="Geneious type: misc"
                     /note="Phob-1"
                     /standard_name="Pho2-2abcd-1 (formerly Phob-1)"
     misc_feature    10493..10568
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10569..10588
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10589..10608
                     /note="Geneious type: misc"
                     /note="Phob-2"
                     /standard_name="Pho2-2abcd-2 (formerly Phob-2)"
     misc_feature    10609..10684
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10685..10704
                     /note="Csy4"
                     /standard_name="Csy4"
     misc_feature    10705..10724
                     /note="Geneious type: misc"
                     /note="Phob-3"
                     /standard_name="Pho2-2abcd-3 (formerly Phob-3)"
     misc_feature    10725..10800
                     /note="gRNA Repeat"
                     /standard_name="gRNA Repeat"
     misc_feature    10801..10820
                     /note="Csy4"
                     /standard_name="Csy4"
     terminator      10834..11024
     misc_feature    11033..12689
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pMOD_C2911 - Fragment 2
                     (concatenated sequence 4)"
     misc_feature    11033..11036
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    11060..11571
                     /note="Geneious type: promoter prokaryotic"
                     /standard_name="rolD promoter"
     misc_feature    11403..11423
                     /note="Geneious type: primer"
                     /standard_name="rolD F1"
     misc_feature    11560..11562
                     /standard_name="start"
     CDS             11587..12297
                     /note="Geneious type: cds"
                     /standard_name="TREX2"
     terminator      12304..12609
     misc_feature    12686..3718
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pTRANS_220 - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    12686..12689
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    12932..12957
                     /note="T-DNA repeat"
                     /standard_name="T-DNA border (R)"
     misc_feature    complement(13999..14999)
                     /standard_name="STA region from pVS1"
     misc_feature    complement(15592..16592)
                     /standard_name="pVS1 ori"
     misc_feature    complement(17002..17262)
                     /standard_name="bom site from pBR322"
     rep_origin      complement(17402..17682)
ORIGIN      
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcggtacc cggggatcgg
     3721 cgcgccagat ttgccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
     3781 cttacgcagc aggtatcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
     3841 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     3901 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     3961 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
     4021 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
     4081 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
     4141 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
     4201 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
     4261 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
     4321 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
     4381 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4441 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4501 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     4561 gggggactcc tgcaggaaaa atggatcatt atcttgatat tagacttaga cctgatccag
     4621 aatttccacc agctcaactt atgtctgttc tttttggaaa acttcatcaa gctcttgttg
     4681 ctcaaggagg agatagaatt ggagtttctt ttcctgatct tgatgaatca agatcaagac
     4741 ttggagaaag acttagaatt catgcttctg ctgatgatct tagagctttg cttgctagac
     4801 cttggcttga aggacttaga gatcatcttc aatttggaga accagctgtt gttccacatc
     4861 caactcctta tagacaagtt tcaagagttc aagctaaatc taatccagaa agacttagaa
     4921 gaagacttat gagaagacat gatctttctg aagaagaagc tagaaaaaga attcctgata
     4981 ctgttgctag agctttggat ttgccttttg ttacacttag atcacaatct actggacaac
     5041 attttagact ttttattaga catggaccac ttcaagttac tgctgaagaa ggaggattta
     5101 cttgttatgg actttctaag ggaggttttg ttccttggtt tggatctgga gctactaatt
     5161 tttctcttct taagcaagct ggagatgttg aagaaaatcc tggacccatg gataagaagt
     5221 actctatcgg actcgatatc ggaactaact ctgtgggatg ggctgtgatc accgatgagt
     5281 acaaggtgcc atctaagaag ttcaaggttc tcggaaacac cgataggcac tctatcaaga
     5341 aaaaccttat cggtgctctc ctcttcgatt ctggtgaaac tgctgaggct accagactca
     5401 agagaaccgc tagaagaagg tacaccagaa gaaagaacag gatctgctac ctccaagaga
     5461 tcttctctaa cgagatggct aaagtggatg attcattctt ccacaggctc gaagagtcat
     5521 tcctcgtgga agaagataag aagcacgaga ggcaccctat cttcggaaac atcgttgatg
     5581 aggtggcata ccacgagaag taccctacta tctaccacct cagaaagaag ctcgttgatt
     5641 ctactgataa ggctgatctc aggctcatct acctcgctct cgctcacatg atcaagttca
     5701 gaggacactt cctcatcgag ggtgatctca accctgataa ctctgatgtg gataagttgt
     5761 tcatccagct cgtgcagacc tacaaccagc ttttcgaaga gaaccctatc aacgcttcag
     5821 gtgtggatgc taaggctatc ctctctgcta ggctctctaa gtcaagaagg cttgagaacc
     5881 tcattgctca gctccctggt gagaagaaga acggactttt cggaaacttg atcgctctct
     5941 ctctcggact cacccctaac ttcaagtcta acttcgatct cgctgaggat gcaaagctcc
     6001 agctctcaaa ggatacctac gatgatgatc tcgataacct cctcgctcag atcggagatc
     6061 agtacgctga tttgttcctc gctgctaaga acctctctga tgctatcctc ctcagtgata
     6121 tcctcagagt gaacaccgag atcaccaagg ctccactctc agcttctatg atcaagagat
     6181 acgatgagca ccaccaggat ctcacacttc tcaaggctct tgttagacag cagctcccag
     6241 agaagtacaa agagattttc ttcgatcagt ctaagaacgg atacgctggt tacatcgatg
     6301 gtggtgcatc tcaagaagag ttctacaagt tcatcaagcc tatcctcgag aagatggatg
     6361 gaaccgagga actcctcgtg aagctcaata gagaggatct tctcagaaag cagaggacct
     6421 tcgataacgg atctatccct catcagatcc acctcggaga gttgcacgct atccttagaa
     6481 ggcaagagga tttctaccca ttcctcaagg ataacaggga aaagattgag aagattctca
     6541 ccttcagaat cccttactac gtgggacctc tcgctagagg aaactcaaga ttcgcttgga
     6601 tgaccagaaa gtctgaggaa accatcaccc cttggaactt cgaagaggtg gtggataagg
     6661 gtgctagtgc tcagtctttc atcgagagga tgaccaactt cgataagaac cttccaaacg
     6721 agaaggtgct ccctaagcac tctttgctct acgagtactt caccgtgtac aacgagttga
     6781 ccaaggttaa gtacgtgacc gagggaatga ggaagcctgc ttttttgtca ggtgagcaaa
     6841 agaaggctat cgttgatctc ttgttcaaga ccaacagaaa ggtgaccgtg aagcagctca
     6901 aagaggatta cttcaagaaa atcgagtgct tcgattcagt tgagatttct ggtgttgagg
     6961 ataggttcaa cgcatctctc ggaacctacc acgatctcct caagatcatt aaggataagg
     7021 atttcttgga taacgaggaa aacgaggata tcttggagga tatcgttctt accctcaccc
     7081 tctttgaaga tagagagatg attgaagaaa ggctcaagac ctacgctcat ctcttcgatg
     7141 ataaggtgat gaagcagttg aagagaagaa gatacactgg ttggggaagg ctctcaagaa
     7201 agctcattaa cggaatcagg gataagcagt ctggaaagac aatccttgat ttcctcaagt
     7261 ctgatggatt cgctaacaga aacttcatgc agctcatcca cgatgattct ctcaccttta
     7321 aagaggatat ccagaaggct caggtttcag gacagggtga tagtctccat gagcatatcg
     7381 ctaacctcgc tggatctcct gcaatcaaga agggaatcct ccagactgtg aaggttgtgg
     7441 atgagttggt gaaggtgatg ggaaggcata agcctgagaa catcgtgatc gaaatggcta
     7501 gagagaacca gaccactcag aagggacaga agaactctag ggaaaggatg aagaggatcg
     7561 aggaaggtat caaagagctt ggatctcaga tcctcaaaga gcaccctgtt gagaacactc
     7621 agctccagaa tgagaagctc tacctctact acctccagaa cggaagggat atgtatgtgg
     7681 atcaagagtt ggatatcaac aggctctctg attacgatgt tgatcatatc gtgccacagt
     7741 cattcttgaa ggatgattct atcgataaca aggtgctcac caggtctgat aagaacaggg
     7801 gtaagagtga taacgtgcca agtgaagagg ttgtgaagaa aatgaagaac tattggaggc
     7861 agctcctcaa cgctaagctc atcactcaga gaaagttcga taacttgact aaggctgaga
     7921 ggggaggact ctctgaattg gataaggcag gattcatcaa gaggcagctt gtggaaacca
     7981 ggcagatcac taagcacgtt gcacagatcc tcgattctag gatgaacacc aagtacgatg
     8041 agaacgataa gttgatcagg gaagtgaagg ttatcaccct caagtcaaag ctcgtgtctg
     8101 atttcagaaa ggatttccaa ttctacaagg tgagggaaat caacaactac caccacgctc
     8161 acgatgctta ccttaacgct gttgttggaa ccgctctcat caagaagtat cctaagctcg
     8221 agtcagagtt cgtgtacggt gattacaagg tgtacgatgt gaggaagatg atcgctaagt
     8281 ctgagcaaga gatcggaaag gctaccgcta agtatttctt ctactctaac atcatgaatt
     8341 tcttcaagac cgagattacc ctcgctaacg gtgagatcag aaagaggcca ctcatcgaga
     8401 caaacggtga aacaggtgag atcgtgtggg ataagggaag ggatttcgct accgttagaa
     8461 aggtgctctc tatgccacag gtgaacatcg ttaagaaaac cgaggtgcag accggtggat
     8521 tctctaaaga gtctatcctc cctaagagga actctgataa gctcattgct aggaagaagg
     8581 attgggaccc taagaaatac ggtggtttcg attctcctac cgtggcttac tctgttctcg
     8641 ttgtggctaa ggttgagaag ggaaagagta agaagctcaa gtctgttaag gaacttctcg
     8701 gaatcactat catggaaagg tcatctttcg agaagaaccc aatcgatttc ctcgaggcta
     8761 agggatacaa agaggttaag aaggatctca tcatcaagct cccaaagtac tcactcttcg
     8821 aactcgagaa cggtagaaag aggatgctcg cttctgctgg tgagcttcaa aagggaaacg
     8881 agcttgctct cccatctaag tacgttaact ttctttacct cgcttctcac tacgagaagt
     8941 tgaagggatc tccagaagat aacgagcaga agcaactttt cgttgagcag cacaagcact
     9001 acttggatga gatcatcgag cagatctctg agttctctaa aagggtgatc ctcgctgatg
     9061 caaacctcga taaggtgttg tctgcttaca acaagcacag agataagcct atcagggaac
     9121 aggcagagaa catcatccat ctcttcaccc ttaccaacct cggtgctcct gctgctttca
     9181 agtacttcga tacaaccatc gataggaaga gatacacctc taccaaagaa gtgctcgatg
     9241 ctaccctcat ccatcagtct atcactggac tctacgagac taggatcgat ctctcacagc
     9301 tcggtggtga ttcaagggct gatcctaaga agaagaggaa ggtttgacgt cgacgatatg
     9361 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     9421 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     9481 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     9541 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     9601 ataagccaga gctctggtga cggacggcgc gctggcagac atactgtccc acaaatgaag
     9661 atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc
     9721 ctttaatcaa taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct
     9781 ttttctgacg aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat
     9841 cttcagactc caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt
     9901 gggaaatgtc cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg
     9961 agcaaaatct cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt
    10021 caaggcggcg aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac
    10081 aggtaagagc taagcttcct gcaggttcac tgccgtatag gcagattaga acctaaagga
    10141 accggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
    10201 aagtggcacc gagtcggtgc gttcactgcc gtataggcag aattgggaag ataaagacca
    10261 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    10321 ggcaccgagt cggtgcgttc actgccgtat aggcaggttt gagtctccta aatacggttt
    10381 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
    10441 ccgagtcggt gcgttcactg ccgtataggc agattatgta actaaggggc cagttttaga
    10501 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    10561 gtcggtgcgt tcactgccgt ataggcagtc acacgcaaac tggcagctgt tttagagcta
    10621 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
    10681 gtgcgttcac tgccgtatag gcaggttcaa aggccgctcg aggagtttta gagctagaaa
    10741 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
    10801 gttcactgcc gtataggcag gtcgatcgac aagctcgagt ttctccataa taatgtgtga
    10861 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
    10921 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
    10981 cctaaaacca aaatccagta ctaaaatcca gatcccccga attagagctc taccggcgta
    11041 atatggcgcg ccgaagagcg cgaggtcgac ggtatcgata agcttgatat cgaattcctg
    11101 cagcccgggg gatccgcccg ctctagactg ccttgtaggt gcgaattttc tctgctcaaa
    11161 ttgttgaggt tagcggattt gtaaacgcgt ttatatgggc tgcttggagg gtacttttgg
    11221 attaattttt ttctgccagc gcattctgac gcggcaccgc tttggaaagt gcgctgtggg
    11281 tccgcgtttt ctacaataat gtgccgatcc ggtcagaaag tatatggatg agttgtgcca
    11341 gcctcaccaa cgtgctgcag gcccatcatg actacttcaa tgttaatggg ggtaatgaat
    11401 aaataggcga aattgggttc acggtgggcc cagggaatat aatattgccg cagaggtagt
    11461 cggatgccaa ggcccgcaac taatagttca cgaacaaatt cattgtagtg ggcggccaac
    11521 tccaaaacca attgccagtt attgtattgc aatacatata tggcttcctc ccttgcctgc
    11581 aggatgtctg agccacctcg ggctgagacc tttgtattcc tggacctaga agccactggg
    11641 ctcccaaaca tggaccctga gattgcagag atatcccttt ttgctgttca ccgctcttcc
    11701 ctggagaacc cagaacggga tgattctggt tccttggtgc tgccccgtgt tctggacaag
    11761 ctcacactgt gcatgtgccc ggagcgcccc tttactgcca aggccagtga gattactggt
    11821 ttgagcagcg aaagcctgat gcactgcggg aaggctggtt tcaatggcgc tgtggtaagg
    11881 acactgcagg gcttcctaag ccgccaggag ggccccatct gccttgtggc ccacaatggc
    11941 ttcgattatg acttcccact gctgtgcacg gagctacaac gtctgggtgc ccatctgccc
    12001 caagacactg tctgcctgga cacactgcct gcattgcggg gcctggaccg tgctcacagc
    12061 cacggcacca gggctcaagg ccgcaaaagc tacagcctgg ccagtctctt ccaccgctac
    12121 ttccaggctg aacccagtgc tgcccattca gcagaaggtg atgtgcacac cctgcttctg
    12181 atcttcctgc atcgtgctcc tgagctgctc gcctgggcag atgagcaggc ccgcagctgg
    12241 gctcatattg agcccatgta cgtgccacct gatggtccaa gcctcgaagc ctgataactc
    12301 gaggttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt
    12361 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
    12421 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
    12481 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga
    12541 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact
    12601 tgtagttgtg agctcgcgcg tagtcctcgg taatatcgca gaacaaaagt acctgatatc
    12661 gagtgtactt caagtcacac cggcgagtgt ggcactggcc gtcgttttac aacgtcgtga
    12721 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    12781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    12841 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
    12901 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
    12961 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
    13021 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
    13081 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
    13141 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
    13201 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
    13261 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
    13321 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
    13381 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
    13441 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
    13501 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
    13561 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
    13621 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
    13681 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
    13741 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
    13801 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
    13861 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
    13921 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
    13981 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
    14041 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
    14101 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
    14161 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
    14221 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
    14281 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
    14341 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
    14401 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
    14461 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
    14521 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
    14581 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
    14641 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
    14701 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
    14761 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
    14821 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
    14881 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
    14941 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
    15001 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
    15061 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
    15121 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
    15181 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
    15241 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
    15301 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
    15361 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
    15421 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
    15481 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
    15541 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
    15601 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
    15661 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
    15721 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
    15781 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
    15841 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
    15901 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
    15961 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
    16021 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
    16081 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
    16141 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
    16201 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
    16261 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
    16321 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
    16381 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
    16441 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
    16501 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
    16561 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
    16621 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
    16681 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
    16741 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    16801 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    16861 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    16921 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    16981 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    17041 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    17101 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    17161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    17221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    17281 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    17341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    17401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    17461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    17521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    17581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    17641 ctggcagcag ccactggtaa caggattagc agagcgaggt at
//
