LOCUS       pMOD_C2911-Addgene_#161764        3670 bp    DNA     circular UNA 02-OCT-2020
DEFINITION  MODULE C plasmid compatible with the Voytas Plant Genome
            Engineering Tool kit with an Agrobacterium promoter rolD expressing
            the TREX2 exonuclease. .
ACCESSION   urn.local...3je-ch5h1ru
VERSION     urn.local...3je-ch5h1ru
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     cds             6..716
                     /vntifkey=21
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="TREX2"
     terminator      723..1028
                     /%_Identity=100
                     /Motif="GTTCGAGTATTATGGCATTGGGAAAACTGTTTTTCTTGTACCATTTGTTG
                     TGCTTGTAATTTACTGTGTTTTTTATTCGGTTTTCGCTATCGAACTGTGAAATGGAAA
                     TGGATGGAGAAGAGTTAATGAATGATATGGTCCTTTTGTTCATTCTCAAATTAATATT
                     ATTTGTTTTTTCTCTTATTTGTTGTGTGTTGAATTTGAAATTATAAGAGATATGCAAA
                     CATTTTGTTTTGAGTAAAAATGTGTCAAATCGTGGCCTCTAATGACCGAAGTTAATAT
                     GAGGAGTAAAACACTTGTAGTTGT"
                     /annotation_group="pea rbcs: 3,187 -> 3,492"
                     /modified_by="User"
                     /X-Transferred_From="pMOD_C2806; pMOD_C2812"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pMOD_C2806, pMOD_C2812, pMOD_C2813"
                     /Transferred_Similarity="100.00%"
                     /label="pea rbcS E9 terminator"
     overhang        1036..1051
                     /%_Identity=100
                     /Motif="CGCGTAGTCCTCGGTA"
                     /annotation_group="CGCGTAGTCCTCGGTA: 928 -> 943"
                     /modified_by="User"
                     /X-Transferred_From="pMOD_C0000; pMOD_C2200; pMOD_C2515;
                     pMOD_C2516; pMOD_C2517; pMOD_C2518; pMOD_C2519;
                     pMOD_C2520; pMOD_C2616; pMOD_C2806; pMOD_C2812"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pMOD_C0000, pMOD_C2200, pMOD_C2515,
                     pMOD_C2516, pMOD_C2517, pMOD_C2518, pMOD_C2519,
                     pMOD_C2520, pMOD_C2616, pMOD_C2806, pMOD_C2812,
                     pMOD_C2813"
                     /Transferred_Similarity="100.00%"
                     /label="Gibson overlap"
     overhang        1080..1096
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="pMOD_C0000; pMOD_C2200; pMOD_C2515;
                     pMOD_C2516; pMOD_C2517; pMOD_C2518; pMOD_C2519;
                     pMOD_C2520; pMOD_C2616; pMOD_C2806; pMOD_C2812"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pMOD_C0000, pMOD_C2200, pMOD_C2515,
                     pMOD_C2516, pMOD_C2517, pMOD_C2518, pMOD_C2519,
                     pMOD_C2520, pMOD_C2616, pMOD_C2806, pMOD_C2812,
                     pMOD_C2813"
                     /Transferred_Similarity="100.00%"
                     /label="Gibson overlap"
     gene            1135..2188
                     /ApEinfo_fwdcolor="#66ffff; #e9d024"
                     /ApEinfo_revcolor="#66ffff; #e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="pMOD_A0000; pMOD_A0101; pMOD_A0102;
                     pMOD_A0103; pMOD_A0108; pMOD_A0109 - BAD!!!; pMOD_A0111;
                     pMOD_A0201; pMOD_A0202; pMOD_A0208; pMOD_A0301;
                     pMOD_A0302; pMOD_A0308; pMOD_A0309 - BAD!!!; pMOD_A0401;
                     pMOD_A0402; pMOD_A0408; pMOD_A0409 - BAD!!!; pMOD_A0501;
                     pMOD_A0502; pMOD_A0503; pMOD_A0508; pMOD_A0509 - BAD!!!;
                     pMOD_A0601; pMOD_A0602; pMOD_A0608; pMOD_A0609 - BAD!!!;
                     pMOD_A0701; pMOD_A0702; pMOD_A0708; pMOD_A0709 - BAD!!!;
                     pMOD_A0801; pMOD_A0802; pMOD_A0808; pMOD_A0809 - BAD!!!;
                     pMOD_A0901; pMOD_A0902; pMOD_A0908 - BAD!!!; pMOD_A0909 -
                     BAD!!!; pMOD_A1001; pMOD_A1002; pMOD_A1010; pMOD_A1110;
                     pMOD_A1210; pMOD_A1310; pMOD_A1410; pMOD_A1510;
                     pMOD_A1610; pMOD_A1710; pMOD_A1810; pMOD_A1910;
                     pMOD_A3001; pMOD_A3003; pMOD_A3010; pMOD_A3012;
                     pMOD_A3013; pMOD_A3014; pMOD_B0000; pMOD_B2000;
                     pMOD_B2101; pMOD_B2103; pMOD_B2103b; pMOD_B2109;
                     pMOD_B2112; pMOD_B2203; pMOD_B2301; pMOD_B2303;
                     pMOD_B2309; pMOD_B2312; pMOD_B2403; pMOD_B2515;
                     pMOD_B2517; pMOD_B2518; pMOD_B2519; pMOD_B2520;
                     pMOD_B2615; pMOD_C0000; pMOD_C2200; pMOD_C2515;
                     pMOD_C2516; pMOD_C2517; pMOD_C2518; pMOD_C2519;
                     pMOD_C2520; pMOD_C2616"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 99.91%"
                     /%_Identity=100
                     /Motif="GTCAGGTGGCACTTTTCGGGGAAATGTGCGATGAACCCCTATTTGTTTAT
                     TTTTCTAAATACATTCAAATATGTATCCGCTAATGAGACAATAACCCTGATAAATGCT
                     TCAATTATATTGAAAAAGGAAGAGTATGAGTATCCAACATTTCCGTGTCGCCCTTATT
                     CCCTTTTTTGCGGCCTTCTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAG
                     TAAAAGATGCTGAAGATCAGTTGGGTGCCCGAGTGGGTTACATTGAACTGGATCTCAA
                     CAGCGGCAAAATCTTAGAGAGTTTTCGCCCCGAAGAACGCTTCCCAATGATGAGCACT
                     TTCAAAGTTCTGCTATGTGGCGCGGTGTTATCCCGTATTGATGCTGGGCAAGAGCAAC
                     TCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGA
                     AAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGTGCTGCCATAACCATG
                     AGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAA
                     CCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGA
                     GCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
                     ACAACTCTCCGCAAACTATTAACTGGCGAACTACTTACTCTTGCTTCACGCCAGCAAC
                     TCATTGACTGGATGGAGGCGGACAAAGTTGCAGGACCACTTCTGCGCTCGGCACTTCC
                     GGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
                     ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGG
                     GGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACT
                     GATTAAGCATTGGTAACTGTCAGACGAAGGGAAATAAATAGTAGCCCGCCTGATGTGC
                     GGGCTTTTTTTTGGTGGT"
                     /annotation_group="AmpR: 213 -> 1,266"
                     /modified_by="User"
                     /modified_by="User"
                     /%_Identity=100
                     /annotation_group="AmpR: 213 -> 1,266"
                     /Transferred_From="pMOD_C0000, pMOD_C2515, pMOD_C2516,
                     pMOD_C2517, pMOD_C2518, pMOD_C2519, pMOD_C2520,
                     pMOD_C2806, pMOD_C2812, pMOD_C2813, pMOD_C2200,
                     pMOD_C2616"
                     /Transferred_Similarity="100.00% -> 99.91%"
                     /label="AmpR"
     primer          complement(1280..1302)
                     /created_by="Garvin project"
                     /label="ZY015R "
     rep_origin      2256..3112
                     /modified_by="User"
                     /created_by="User"
                     /%_Identity=100
                     /Motif="GCCTTGACGGCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCA
                     GACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCT
                     GCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGA
                     GCTACTAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACT
                     GTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTA
                     CATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTG
                     TCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGA
                     ACGGGGGGTTCGTGCTCACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAT
                     ACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG
                     GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGA
                     AACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT
                     TTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTT
                     TTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCC
                     CCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCA
                     GCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCC"
                     /annotation_group="High Copy Ori: 1 -> 856"
                     /ApEinfo_fwdcolor="#800080; #ff8000"
                     /ApEinfo_revcolor="#ff00ff; #ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="pMOD_A0000; pMOD_A0101; pMOD_A0102;
                     pMOD_A0103; pMOD_A0108; pMOD_A0109 - BAD!!!; pMOD_A0111;
                     pMOD_A0201; pMOD_A0202; pMOD_A0208; pMOD_A0301;
                     pMOD_A0302; pMOD_A0308; pMOD_A0309 - BAD!!!; pMOD_A0401;
                     pMOD_A0402; pMOD_A0408; pMOD_A0409 - BAD!!!; pMOD_A0501;
                     pMOD_A0502; pMOD_A0503; pMOD_A0508; pMOD_A0509 - BAD!!!;
                     pMOD_A0601; pMOD_A0602; pMOD_A0608; pMOD_A0609 - BAD!!!;
                     pMOD_A0701; pMOD_A0702; pMOD_A0708; pMOD_A0709 - BAD!!!;
                     pMOD_A0801; pMOD_A0802; pMOD_A0808; pMOD_A0809 - BAD!!!;
                     pMOD_A0901; pMOD_A0902; pMOD_A0908 - BAD!!!; pMOD_A0909 -
                     BAD!!!; pMOD_A1001; pMOD_A1002; pMOD_A1010; pMOD_A1110;
                     pMOD_A1210; pMOD_A1310; pMOD_A1410; pMOD_A1510;
                     pMOD_A1610; pMOD_A1710; pMOD_A1810; pMOD_A1910;
                     pMOD_A3001; pMOD_A3003; pMOD_A3010; pMOD_A3012;
                     pMOD_A3013; pMOD_A3014; pMOD_B0000; pMOD_B2000;
                     pMOD_B2101; pMOD_B2103; pMOD_B2103b; pMOD_B2109;
                     pMOD_B2112; pMOD_B2203; pMOD_B2301; pMOD_B2303;
                     pMOD_B2309; pMOD_B2312; pMOD_B2403; pMOD_B2515;
                     pMOD_B2517; pMOD_B2518; pMOD_B2519; pMOD_B2520;
                     pMOD_B2615; pMOD_C0000; pMOD_C2200; pMOD_C2515;
                     pMOD_C2516; pMOD_C2517; pMOD_C2518; pMOD_C2519;
                     pMOD_C2520; pMOD_C2616"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 99.88%"
                     /modified_by="User"
                     /Transferred_From="pMOD_C0000, pMOD_C2515, pMOD_C2516,
                     pMOD_C2517, pMOD_C2518, pMOD_C2519, pMOD_C2520,
                     pMOD_C2616, pMOD_C2806, pMOD_C2812, pMOD_C2813,
                     pMOD_C2200"
                     /Transferred_Similarity="100.00% -> 99.88%"
                     /label="High Copy Ori"
     misc_feature    3149..3660
                     /modified_by="Garvin project"
                     /label="rolD promoter"
                     /note="Geneious type: promoter prokaryotic"
     primer          3492..3512
                     /created_by="Garvin project"
                     /label="rolD F1"
     misc_feature    3649..3651
                     /label="start"
     misc_feature    3652..3660
                     /label="ASS enhancer"
     misc_feature    3664
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
ORIGIN      
        1 ggatgtctga gccacctcgg gctgagacct ttgtattcct ggacctagaa gccactgggc
       61 tcccaaacat ggaccctgag attgcagaga tatccctttt tgctgttcac cgctcttccc
      121 tggagaaccc agaacgggat gattctggtt ccttggtgct gccccgtgtt ctggacaagc
      181 tcacactgtg catgtgcccg gagcgcccct ttactgccaa ggccagtgag attactggtt
      241 tgagcagcga aagcctgatg cactgcggga aggctggttt caatggcgct gtggtaagga
      301 cactgcaggg cttcctaagc cgccaggagg gccccatctg ccttgtggcc cacaatggct
      361 tcgattatga cttcccactg ctgtgcacgg agctacaacg tctgggtgcc catctgcccc
      421 aagacactgt ctgcctggac acactgcctg cattgcgggg cctggaccgt gctcacagcc
      481 acggcaccag ggctcaaggc cgcaaaagct acagcctggc cagtctcttc caccgctact
      541 tccaggctga acccagtgct gcccattcag cagaaggtga tgtgcacacc ctgcttctga
      601 tcttcctgca tcgtgctcct gagctgctcg cctgggcaga tgagcaggcc cgcagctggg
      661 ctcatattga gcccatgtac gtgccacctg atggtccaag cctcgaagcc tgataactcg
      721 aggttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
      781 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
      841 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
      901 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
      961 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     1021 gtagttgtga gctcgcgcgt agtcctcggt aatatcgcag aacaaaagta cctgatatcg
     1081 agtgtacttc aagtcacacc ggcgagtgac tagcaggtga tgaaaagcct tagggtcagg
     1141 tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc
     1201 aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag
     1261 gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg
     1321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1381 gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt
     1441 tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt
     1501 gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1561 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1621 agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1681 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1801 cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac
     1861 tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact
     1921 tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1981 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2101 aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg
     2161 cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc
     2221 tcttaggcgg tccgattagc gacatagtca tcaatgcctt gacggcaaaa tcccttaacg
     2281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2401 ggtttgtttg ccggatcaag agctactaac tctttttccg aaggtaactg gcttcagcag
     2461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2641 gcggtcgggc tgaacggggg gttcgtgctc acagcccagc ttggagcgaa cgacctacac
     2701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc cacctgcaac
     3121 gccggcgtaa tatggcgcgc cgaagagcgc gaggtcgacg gtatcgataa gcttgatatc
     3181 gaattcctgc agcccggggg atccgcccgc tctagactgc cttgtaggtg cgaattttct
     3241 ctgctcaaat tgttgaggtt agcggatttg taaacgcgtt tatatgggct gcttggaggg
     3301 tacttttgga ttaatttttt tctgccagcg cattctgacg cggcaccgct ttggaaagtg
     3361 cgctgtgggt ccgcgttttc tacaataatg tgccgatccg gtcagaaagt atatggatga
     3421 gttgtgccag cctcaccaac gtgctgcagg cccatcatga ctacttcaat gttaatgggg
     3481 gtaatgaata aataggcgaa attgggttca cggtgggccc agggaatata atattgccgc
     3541 agaggtagtc ggatgccaag gcccgcaact aatagttcac gaacaaattc attgtagtgg
     3601 gcggccaact ccaaaaccaa ttgccagtta ttgtattgca atacatatat ggcttcctcc
     3661 cttgcctgca
//
