LOCUS       Exported                1727 bp ds-DNA     linear   SYN 26-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 1, 2020 from SnapGene 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    290..400
                     /label=exon 1
                     /note="exon 1"
                     /note="color: #fd8008"
     misc_feature    376..398
                     /label=Cas9_sg1{0
                     /note="Cas9_sg1{0: 1, 1: 1, 2: 2, 3: 12, 4: 105}"
                     /note="color: #fd8008; direction: RIGHT"
     misc_feature    386..1335
                     /label=Cas9_sg2{0: 1, 1: 0, 2: 0, 3: 1, 4: 45}
                     /note="Cas9_sg2{0: 1, 1: 0, 2: 0, 3: 1, 4: 45}"
                     /note="color: #fd8008; direction: RIGHT"
     misc_feature    401..1285
                     /label=HaloTag (codon-optimized)
                     /note="HaloTag (codon-optimized)"
                     /note="color: #8000ff"
     misc_feature    1286..1327
                     /label=HaloTag linker
                     /note="HaloTag linker (3XG4S)"
                     /note="color: #8000ff"
     misc_feature    1328..1334
                     /label=exon 1
                     /note="exon 1"
                     /note="color: #fd8008"
ORIGIN
        1 ccagccgccg ggaccgcccc ctcgccccgc ccccagcggg cactcaggcc gtaccactgt
       61 gccttcatgg gggtggagat agatcgtggg ctagtcctgc cgaggagaga ggggttcttc
      121 ctcaaaaaat atgattatgt atagtattcg catgattcta gttaacttgt ttcccttctg
      181 cctgctcgga ccctctacct gccctacgaa gggggcggag tgcgttcctg cctccccctg
      241 ctcttccgcg tttggtgcgc gcctgcgcgg tgcgtaggcg gcggagcgta cttaagcttc
      301 gacgcaggag gcggggctgc tcagtcctcc aggcgtcggt actcagcggt gttggaactt
      361 cgttgcttgc ttgcctgtgc gcgcgtgcgc ggacatggcc gagatcggca ccggctttcc
      421 cttcgatcct cactacgtgg aagtgctggg cgagagaatg cactatgtgg acgtgggccc
      481 cagagatgga acccctgtgc tgtttctgca cggcaaccct accagcagct acgtgtggcg
      541 gaacatcatc cctcacgtgg cccctacaca cagatgtatc gcccctgacc tgatcggcat
      601 gggcaagagc gataagcccg acctgggcta cttcttcgac gaccacgtgc ggttcatgga
      661 cgcctttatt gaggccctcg gcctggaaga ggtggtgctg gttattcacg attggggcag
      721 cgccctggga ttccactggg ccaagagaaa ccccgagaga gtgaagggaa tcgccttcat
      781 ggaattcatc aggcccattc ctacctggga cgagtggcct gagttcgcca gagagacatt
      841 ccaggccttc agaaccaccg acgtgggcag aaagctgatc atcgaccaga acgtgttcat
      901 cgagggcacc ctgcctatgg gagtcgtcag accactgacc gaggtggaaa tggaccacta
      961 cagagagccc tttctgaacc ccgtggaccg ggaacctctt tggagattcc ccaacgagct
     1021 gcccattgcc ggcgagcctg ctaatattgt ggccctggtg gaagagtaca tggactggct
     1081 gcatcagagc cccgtgccta agctgctgtt ttggggaaca cccggcgtgc tgattcctcc
     1141 tgctgaagct gccagactgg ccaagagcct gcctaattgc aaggccgtgg atatcggccc
     1201 tggcctgaat ctgctgcaag aggacaaccc cgatctgatc ggctccgaga ttgccagatg
     1261 gctgagcacc ctggaaatca gcggaggagg aggttctgga ggcggtggaa gtggtggcgg
     1321 aggtagctca aacggtaggt aagggcgcga ggcgacggcg gcggcgcacc cggccgaggc
     1381 ctcccagctg ggcttttcgt tttcagtggg accggggcgg cgatcccgtg tgggattttt
     1441 tggcgcccct gtggcgggaa gccgcggaga agagtaactg gaggaggctg gtgtcgccat
     1501 tttgtttcgc tcctctggcc ctcgcgcgcg gggcgggaag tcttttcttt gcagtccgtt
     1561 tgcttggggt gggcgttggg agggacgctt cttaggggtt tgaagcgtca ggtgagggtg
     1621 gaaaacgccc attctccgtg gcctcgcctc ccccaactcc cggccccgcg ctcgagcccg
     1681 ctttgtcgca gtgctgcatc cgggcactcg cggcgcgcac gcgctct
//
