LOCUS       Exported                7593 bp ds-DNA     circular SYN 02-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     1..34
                     /label=LoxP
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     239..260
                     /label=HSV TK p(A)-RV
     CDS             complement(367..1077)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     377..386
                     /label=mCherry RP
     misc_feature    complement(1114..1235)
                     /label=p10 promoter
     primer_bind     complement(1197..1216)
                     /label=p10-FW
     promoter        1275..1366
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1299..1318
                     /label=bac1
     CDS             1419..1445
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1459..1477
                     /label=MSC 1
     primer_bind     complement(1517..1536)
                     /label=SV40polyA
     polyA_signal    1623..1757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1876..3233
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1892..3233)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      complement(3385..3825)
                     /direction=LEFT
                     /label=F1 ori
     promoter        4117..4221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4222..5082
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5253..5841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     6404..7231
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6404..6528
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6566..7171)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcttac ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
      421 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac
      481 gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc
      541 gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc
      601 gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac
      661 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      721 ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg
      781 ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg
      841 cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg
      901 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc
      961 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
     1021 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatacc
     1081 tcctggctcg agatcccggg tgatcaagtc ttcgtcgagt gattgtaaat aaaatgtaat
     1141 ttacagtata gtattttaat taatatacaa atgatttgat aataattctt atttaactat
     1201 aatatattgt gttgggttga attaaaggtc cgtatactag tatcgattcg cgacctactc
     1261 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     1321 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     1381 ataccgtccc accatcgggc gcggatcccg gtccgatgta cccatacgat gttccagatt
     1441 acgctcttat ggccatggcg gccgctttcg aatctagagc ctgcagtctc gacaagcttg
     1501 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1561 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1621 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1681 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1741 cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc
     1801 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggc
     1861 gattaattga tccgggttat tagtacattt attaagcgct agattctgtg cgttgttgat
     1921 ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt
     1981 atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaaat
     2041 cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt ttttccgaac
     2101 cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc aaatcttgta
     2161 gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt
     2221 cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg tacaattgac
     2281 tcgacgtaaa cacgttaaat aaagcttgga catatttaac atcgggcgtg ttagctttat
     2341 taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc gaagacgatt
     2401 ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg atcaaatttg
     2461 tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt ttcaatctaa
     2521 ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc ggtggtaaca
     2581 tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa tctaccatcg
     2641 gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc ggtgatgcag
     2701 acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca actattgtac
     2761 tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt ttttcgtttc
     2821 taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga ggttccgtcg
     2881 gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt ggaggcgctg
     2941 gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt tgttctggtt
     3001 tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc acaacggaag
     3061 gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa ttggaataca
     3121 aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg ccgatattta
     3181 acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc ggatcgatcc
     3241 cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg agacacttcg
     3301 ctgtcgtcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3361 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     3601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3721 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     3841 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3901 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3961 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4021 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4081 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4141 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4201 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4441 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6001 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6061 ccgattcatt aatgcagaac ggctccgccc actattaatg aaattaaaaa ttccaatttt
     6121 aaaaaacgca gcaagagaaa catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc
     6181 gtcgacatgc tgaacaacaa gattaatatg cctccgtgta taaaaaaaat attgaacgat
     6241 ttgaaagaaa acaatgtacc gcgcggcggt atgtacagga agaggtttat actaaactgt
     6301 tacattgcaa acgtggtttc gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg
     6361 acgcatttct acaaccacga ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa
     6421 tcccaagatg tgtataaacc accaaactgc caaaaaatga aaactgtcga caagctctgt
     6481 ccgtttgctg gcaactgcaa gggtctcaat cctatttgta attattgaat aataaaacaa
     6541 ttataaatgc taaatttgtt ttttattaac gatacaaacc aaacgcaaca agaacatttg
     6601 tagtattatc tataattgaa aacgcgtagt tataatcgct gaggtaatat ttaaaatcat
     6661 tttcaaatga ttcacagtta atttgcgaca atataatttt attttcacat aaactagacg
     6721 ccttgtcgtc ttcttcttcg tattccttct ctttttcatt tttctcctca taaaaattaa
     6781 catagttatt atcgtatcca tatatgtatc tatcgtatag agtaaatttt ttgttgtcat
     6841 aaatatatat gtctttttta atggggtgta tagtaccgct gcgcatagtt tttctgtaat
     6901 ttacaacagt gctattttct ggtagttctt cggagtgtgt tgctttaatt attaaattta
     6961 tataatcaat gaatttggga tcgtcggttt tgtacaatat gttgccggca tagtacgcag
     7021 cttcttctag ttcaattaca ccatttttta gcagcaccgg attaacataa ctttccaaaa
     7081 tgttgtacga accgttaaac aaaaacagtt cacctccctt ttctatacta ttgtctgcga
     7141 gcagttgttt gttgttaaaa ataacagcca ttgtaatgag acgcacaaac taatatcaca
     7201 aactggaaat gtctatcaat atatagttgc tgatatccca ttttaccacc ataatactta
     7261 aaatgatcca caaacaagcc accaccaatc gctacactca ccgccgctac agacgctaca
     7321 atcacctcat atttattctc ctcacatatt tattctattc taattctcaa tcataccaat
     7381 caacttctca tactcaatca taccaatcaa tacacattat actactactc atttccaatc
     7441 attcacattc ataatatatc tgttatacta ctccttatca ctactctctc catccacatt
     7501 attatcctcc atcatacgac gctacataac tacgtggttc ctaactacac caagcatagc
     7561 cctagccagc cagccacacc ttacagcctt ttt
//
