LOCUS       Exported                8889 bp ds-DNA     circular SYN 05-OCT-2020
DEFINITION  Campeau blast R4-R3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     The 42-2 promoterless vector was digested in a 3ug reaction with 
            BstX1 in Buffer 3 for 2 hours.  This method was used instead of the 
            EcoRV partial digest since this method failed to produce good 
            results when used for the other Campeau vectors.  This digest was 
            cleaned, and gel confirmed.
            This linearized vector was then blunted, using the NEB Quick 
            Blunting kit to remove the 3' overhands left from the digest.  19ul 
            of the cleaned digest was used in the standard protocol.  
            This blunted vector backbone was then used in a ligation reaction, 
            with 1ul of the vector and 3ul of the pSpcRFA43 EcoRV cassette.  A 
            control igation was also done, using 1ul of the backbone and no 
            insert.  Both ligations were Xfed into DB3.1 cells and plated on A/C
            at 30degrees. 11/3/2009  JM
            There were 2 colonies on the ligation plate, and 0 on the control 
            plate. These colonies were picked. 11/4/2009 JM  Both colonies are 
            the correct size, and will be digested for directionality. 11/5/2009
             JM
            Both clones digested with EcoR1 to check for directionality.  Both 
            appear to be in the forward orientation, and will be sent to 
            sequencing to confirm. 11/6/2009 JM
            Clone 1 was sequence confirmed.  11/19/2009
            A maxi prep was done and filed. 11/19/2009 JM
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             231..411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1796..1912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1944..2067
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2108..2413)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2755..3414)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3468..3498)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3523..3646)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3675..4263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4146..4157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4319..4649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4500..4635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4697..4744
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4763..5161
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5413..5646
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5718..5839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5858..5993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6026..6044)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6054..6070)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6215..6670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6699..6803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6804..7664
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7835..8423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8711..8732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8747..8777
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8785..8801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8809..8825
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8846..8864
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac gggtctctct
      241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      421 gaacagggac ctgaaagcga aagggaaacc agagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcctca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg ttaactttta
     1801 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     1861 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca
     1921 gtgtggtgga attctgcaga tatcaacttt gtatagaaaa gttgaacgag aaacgtaaaa
     1981 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     2041 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
     2101 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
     2161 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
     2221 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggggactt
     2281 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
     2341 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
     2401 ccttaaactg catttcacca gcccctgttc tcgtcggcaa aagagccgtt catttcaata
     2461 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga
     2521 cgacgggctt cattctgcat ggttgtgctt accgaaccgg agatattgac atcatatatg
     2581 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat
     2641 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
     2701 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatcctc tagattacgc
     2761 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     2821 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2881 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2941 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3001 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3061 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3121 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3181 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     3241 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3301 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     3361 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     3421 ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat tatacgagcc
     3481 ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gccatagtga ctggatatgt
     3541 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     3601 tttatatcat tttacgtttc tcgttcaact ttattataca tagttgatat ccagcacagt
     3661 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3721 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3781 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3841 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3901 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3961 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4021 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4081 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4141 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4201 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4261 cgcctggaat tctgcagata tccggttagt aatgagtttg gaattaattc tgtggaatgt
     4321 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4561 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4621 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4681 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     4741 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     4801 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4861 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     4921 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     4981 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5041 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5101 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5161 agcacaattc gagctcggct gggtcgcggg agggccggtc gcagtgattc aggcccttct
     5221 ggattgtgtt ggtccccagg gcacgattgt catgcccacg cactcgggtg atctgactga
     5281 tcccgcagat tggagatcgc cgcccgtgcc tgccgattgg gtgcagatcc gtcgagggcc
     5341 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5401 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     5461 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc tgcctaggga
     5521 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5581 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5641 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5701 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5761 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5821 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     5881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5941 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6001 ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6061 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6121 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6181 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc
     6241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6661 tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7681 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7741 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7981 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8461 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8521 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8581 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8641 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8701 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8761 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8821 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8881 tgcaagctt
//
