LOCUS       Exported                9681 bp ds-DNA     circular SYN 05-OCT-2020
DEFINITION  Multisite R4-R3 Campeau hygromycin vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 7, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     w12-4 promoterless vector from Campeau was Xfed into Stbl3 cells and
            plated on Amp at 30degrees. 10/5/2009 JLM
            There was a lawn on the plate, as so vector was streaked out. 
            10/6/2009 JM
            2 colonies were picked and Qiagen mini prepped.  They were size 
            confirmed, and one clone was saved along with its corresponding 
            glycerol stock. 10/8/2009  JM
            An EcoR5 digest was done on the 745-2 DNA, using 6ul of the DNA, 
            0.5ul of the enzyme and a digest time of 15 minutes at 37 degrees.  
            This digest was run on a gel, but the band is running smaller than 
            the nicked band, so this is telling me that it might have been a 
            complete digest.  
            669.1 checks out by sequencing, plate 108703, 10/22/2009, wells 
            G08/G09.  Changes in the RRE region were added by DE on 10/27.  
            Sequence file is confirmed through attR regions.  DE 10/27/09
            This digest was repeated, using 8ul of the DNA and a 1:10 amount of 
            enzyme for 15 minutes at 37 degrees.  However, when this was run out
            on a gel, there is a small amount of nicked, which is encouraging, 
            but there is also still a fair amount of completely digested 
            material.  With the larger amount of DNA used to start, you can 
            clearly see the faint band at 650bp, indicating that the woodchuck 
            element had been popped out.  So, this nicked band could be gel 
            extracted. 10/9/2009 JM
            A BstX1 digest with w12-4 was done, using 15ul of the vector, and 
            digesting at 37 degrees for 1 hour.  This was done as a back-up, 
            since the partial digests are not working properly. This digest 
            properly linearized the vector, and was therefore cleaned. 
            10/13/2009 JM
            The BstX1 digest was cleaned again, this time MinEluting in 15ul of 
            TE in order to concentrate the digest.  The NEB Quick Blunting kit 
            was used to treat the ends of this linearized vector.  The protocol 
            from the kit was followed, using 12ul of the digest along with 7ul 
            of water.  The heat inactivated sample was then used for ligations.
            A ligation was done using 2ul of the blunted w12-4 BstX1 vector and 
            4ul of the pSpcRFA43 EcoR5 cut cassette.  This  ligation was Xfed 
            into DB3.1 cells and plated on Amp/Chlor plates at 30degrees.  
            10/14/2009  JM
            There were 3 colonies on this plate. 10/15/2009  JM  All 3 colonies 
            were picked. 10/19/2009  JM
            All were prepped and run on a gel to verify sizes. They were all the
            correct size, and were digested with Pst1 to check for 
            directionality. 10/20/2009  JM  There were 2 forward clones and 1 
            reverse.  Clones 1 and 2 were sent to sequencing, as these were the 
            forward ones. 10/20/2009  JM
            Clone 669.1 was sequence confirmed.   Maxi prep was done of the 
            confirmed clone. 10/28/2009  JM
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4012..4128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4140..4263
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4304..4609)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4951..5610)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5664..5694)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5719..5842)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5861..6449
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6332..6343)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6505..7004
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    7022..7837
                     /label=HygR
     LTR             8422..8655
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8727..8848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8867..9002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9035..9053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9063..9079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9224..9679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgtccagatc
     4141 aactttgtat agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     4201 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     4261 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     4321 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     4381 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     4441 cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg
     4501 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     4561 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     4621 ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     4681 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     4741 gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     4801 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     4861 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     4921 tctggctttt agtaagccgg atcctctaga ttacgccccg ccctgccact catcgcagta
     4981 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     5041 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     5101 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     5161 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     5221 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     5281 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     5341 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     5401 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     5461 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     5521 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     5581 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     5641 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
     5701 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     5761 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     5821 tcaactttat tatacatagt tgatgtggcg gccgctcgac aatcaacctc tggattacaa
     5881 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5941 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6001 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6061 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6121 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6181 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6241 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6301 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6361 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6421 tcggatctcc ctttgggccg cctccccgcc tggaattctg cagatatccg gactagtgat
     6481 ctctcgaggt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     6541 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     6601 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     6661 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     6721 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6781 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6841 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6901 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6961 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atcccgccac
     7021 catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     7081 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     7141 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     7201 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     7261 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     7321 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     7381 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     7441 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     7501 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     7561 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     7621 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     7681 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     7741 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7801 gcggctccgg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     7861 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     7921 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     7981 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     8041 aggaatagag tagatgccga ccgaacaaga gctgatttcg agaacgcctc agccagcaac
     8101 tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg gcacagttct
     8161 cgtccacagt tcgctaagct cgctcggctg ggtcgcggga gggccggtcg cagtgattca
     8221 ggcccttctg gattgtgttg gtccccaggg cacgattgtc atgcccacgc actcgggtga
     8281 tctgactgat cccgcagatt ggagatcgcc gcccgtgcct gccgattggg tgcagatccg
     8341 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8401 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     8461 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8521 gcctagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8581 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8641 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8701 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8821 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8941 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9001 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9061 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9181 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     9241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9661 atattaacgt ttacaatttc c
//
