LOCUS       pEGFP-N1-HSD17B11-S172L-FLAGC        5623 bp    DNA     circular      7-AUG-2020
SOURCE      
  ORGANISM  
COMMENT     Created by Sébastien Britton
FEATURES             Location/Qualifiers
     rep_origin      4342..4961
                     /vntifkey="33"
                     /label=pBR322\Ori
     promoter        5070..5622
                     /vntifkey="29"
                     /label=CMV\Promoter
     misc_feature    967..990
                     /vntifkey="21"
                     /label=FLAGC
     polyA_signal    3986..4183
                     /vntifkey="25"
                     /label=HSV\TK\PolyA
     promoter        2772..2907
                     /vntifkey="29"
                     /label=SV40\Promoter
     misc_feature    1739..1967
                     /vntifkey="21"
                     /label=SV40\PolyA
     CDS             2956..3750
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=Kana/NeoR
     CDS             1006..1725
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /db_xref="GI:1377912"
                     /vntifkey="4"
                     /label=EGFP
     gene            1006..1725
                     /gene="egfp"
                     /vntifkey="60"
     misc_feature    61..960
                     /vntifkey="21"
                     /label=HSD17B11
     misc_feature    574..576
                     /vntifkey="21"
                     /label=S172L
BASE COUNT     1406 a      1451 c      1443 g      1323 t 
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagccacc 
       61 atgaaatttc ttctggacat cctcctgctt ctcccgttac tgatcgtctg ctccctagag 
      121 tccttcgtga agctttttat tcctaagagg agaaaatcag tcaccggcga aatcgtgctg 
      181 attacaggag ctgggcatgg aattgggaga ctgactgcct atgaatttgc taaacttaaa 
      241 agcaagctgg ttctctggga tataaataag catggactgg aggaaacagc tgccaaatgc 
      301 aagggactgg gtgccaaggt tcataccttt gtggtagact gcagcaaccg agaagatatt 
      361 tacagctctg caaagaaggt gaaggcagaa attggagatg ttagtatttt agtaaataat 
      421 gctggtgtag tctatacatc agatttgttt gctacacaag atcctcagat tgaaaagact 
      481 tttgaagtta atgtacttgc acatttctgg actacaaagg catttcttcc tgcaatgacg 
      541 aagaataacc atggccatat tgtcactgtg gctttggcag ctggacatgt ttcggtcccc 
      601 ttcttactgg cttactgttc aagcaagttt gctgctgttg gatttcataa aactttgaca 
      661 gatgaactgg ctgccttaca aataactgga gtcaaaacaa catgtctgtg tcctaatttc 
      721 gtaaacactg gcttcatcaa aaatccaagt acaagtttgg gacccactct ggaacctgag 
      781 gaagtggtaa acaggctgat gcatgggatt ctgactgagc agaagatgat ttttattcca 
      841 tcttctatag cttttttaac aacattggaa aggatccttc ctgagcgttt cctggcagtt 
      901 ttaaaacgaa aaatcagtgt taagtttgat gcagttattg gatataaaat gaaagcgcaa 
      961 acgcgtgatt acaaggatga cgacgataag ccaccggtcg ccaccatggt gagcaagggc 
     1021 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 
     1081 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 
     1141 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 
     1201 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 
     1261 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 
     1321 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 
     1381 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 
     1441 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 
     1501 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 
     1561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 
     1621 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 
     1681 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct 
     1741 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 
     1801 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 
     1861 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     1921 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 
     1981 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 
     2041 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 
     2101 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 
     2161 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 
     2221 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 
     2281 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 
     2341 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 
     2401 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 
     2461 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     2521 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 
     2581 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     2641 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     2701 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     2761 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     2821 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 
     2881 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 
     2941 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 
     3001 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 
     3061 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 
     3121 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 
     3181 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 
     3241 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 
     3301 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 
     3361 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 
     3421 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 
     3481 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 
     3541 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 
     3601 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 
     3661 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 
     3721 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 
     3781 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 
     3841 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 
     3901 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 
     3961 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 
     4021 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 
     4081 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 
     4141 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 
     4201 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     4261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     4321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     4381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     4441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     4501 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     4561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     4621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     4681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     4741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     4801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     4861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     4921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     4981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     5041 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat 
     5101 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     5161 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     5221 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 
     5281 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     5341 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     5401 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     5461 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     5521 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     5581 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gca 
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