LOCUS       pEGFP-N1-HPGD-FLAGC        5521 bp    DNA     circular     16-MAR-2018
SOURCE      
  ORGANISM  
COMMENT     Created by Sébastien Britton
FEATURES             Location/Qualifiers
     misc_feature    11..808
                     /vntifkey="21"
                     /label=HPGD
     gene            854..1573
                     /gene="egfp"
                     /vntifkey="60"
     CDS             854..1573
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /db_xref="GI:1377912"
                     /vntifkey="4"
                     /label=EGFP
     CDS             2804..3598
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=Kana/NeoR
     misc_feature    1587..1815
                     /vntifkey="21"
                     /label=SV40\PolyA
     promoter        2620..2755
                     /vntifkey="29"
                     /label=SV40\Promoter
     polyA_signal    3834..4031
                     /vntifkey="25"
                     /label=HSV\TK\PolyA
     misc_feature    815..838
                     /vntifkey="21"
                     /label=FLAGC
     promoter        4918..5470
                     /vntifkey="29"
                     /label=CMV\Promoter
     rep_origin      4190..4809
                     /vntifkey="33"
                     /label=pBR322\Ori
BASE COUNT     1389 a      1427 c      1436 g      1269 t 
ORIGIN
        1 tcgagccacc atgcacgtga acggcaaagt ggcgctggtg accggcgcgg ctcagggcat 
       61 aggcagagcc tttgcagagg cgctgctgct taagggcgcc aaggtagcgc tggtggattg 
      121 gaatcttgaa gcaggtgtac agtgtaaagc tgccctggat gagcagtttg aacctcagaa 
      181 gactctgttc atccagtgcg atgtggctga ccagcaacaa ctgagagaca cttttagaaa 
      241 agttgtagac cactttggaa gactggacat tttggtcaat aatgctggag tgaataatga 
      301 gaaaaactgg gaaaaaactc tgcaaattaa tttggtttct gttatcagtg gaacctatct 
      361 tggtttggat tacatgagta agcaaaatgg aggtgaaggc ggcatcatta tcaatatgtc 
      421 atctttagca ggactcatgc ccgttgcaca gcagccggtt tattgtgctt caaagcatgg 
      481 catagttgga ttcacacgct cagcagcgtt ggctgctaat cttatgaaca gtggtgtgag 
      541 actgaatgcc atttgtccag gctttgttaa cacagccatc cttgaatcaa ttgaaaaaga 
      601 agaaaacatg ggacaatata tagaatataa ggatcatatc aaggatatga ttaaatacta 
      661 tggaattttg gacccaccat tgattgccaa tggattgata acactcattg aagatgatgc 
      721 tttaaatggt gctattatga agatcacaac ttctaaggga attcattttc aagactatga 
      781 tacaactcca tttcaagcaa aaacccaaac gcgtgattac aaggatgacg acgataagcc 
      841 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 
      901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 
      961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 
     1021 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 
     1081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 
     1141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 
     1201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 
     1261 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 
     1321 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 
     1381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 
     1441 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 
     1501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 
     1561 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag 
     1621 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 
     1681 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     1741 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     1801 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 
     1861 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 
     1921 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 
     1981 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 
     2041 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 
     2101 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 
     2161 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 
     2221 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 
     2281 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     2341 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
     2401 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 
     2461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     2521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
     2581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 
     2641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     2701 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
     2761 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 
     2821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 
     2881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 
     2941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 
     3001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 
     3061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 
     3121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 
     3181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 
     3241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 
     3301 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 
     3361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 
     3421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 
     3481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 
     3541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 
     3601 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 
     3661 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 
     3721 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 
     3781 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 
     3841 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 
     3901 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 
     3961 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 
     4021 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 
     4081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     4141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     4201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     4261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     4321 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 
     4381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     4441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     4501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     4561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     4621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     4681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     4741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     4801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
     4861 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata 
     4921 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 
     4981 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 
     5041 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 
     5101 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 
     5161 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 
     5221 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 
     5281 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 
     5341 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 
     5401 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 
     5461 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat 
     5521 c 
//
