LOCUS       pE-N1-RDH11             4925 bp    DNA     circular     23-JUN-2019
SOURCE      
  ORGANISM  
COMMENT     Created by Sébastien Britton
FEATURES             Location/Qualifiers
     gene            1026..1028
                     /gene="egfp"
                     /vntifkey="60"
     CDS             2259..3053
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=Kana/NeoR
     polyA_signal    1042..1270
                     /vntifkey="25"
                     /label=SV40\PolyA
     polyA_signal    3289..3486
                     /vntifkey="25"
                     /label=HSV\TK\PolyA
     rep_origin      3645..4264
                     /vntifkey="33"
                     /label=pBR322\Ori
     misc_feature    4373..4925
                     /vntifkey="21"
                     /label=CMV\Promoter
     misc_feature    complement(1411..1717)
                     /vntifkey="21"
                     /label=f1\Origin
     misc_feature    1908..2176
                     /vntifkey="21"
                     /label=SV40\Promoter
     misc_feature    72..1025
                     /vntifkey="21"
                     /label=RDH11
BASE COUNT     1198 a      1265 c      1278 g      1184 t 
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca 
       61 agcttgccac catggttgag ctcatgttcc cgctgttgct cctccttctg cccttccttc 
      121 tgtatatggc tgcgccccaa atcaggaaaa tgctgtccag tggggtgtgt acatcaactg 
      181 ttcagcttcc tgggaaagta gttgtggtca caggagctaa tacaggtatc gggaaggaga 
      241 cagccaaaga gctggctcag agaggagctc gagtatattt agcttgccgg gatgtggaaa 
      301 agggggaatt ggtggccaaa gagatccaga ccacgacagg gaaccagcag gtgttggtgc 
      361 ggaaactgga cctgtctgat actaagtcta ttcgagcttt tgctaagggc ttcttagctg 
      421 aggaaaagca cctccacgta ttgatcaaca atgcaggagt gatgatgtgt ccgtactcga 
      481 agacagcaga tggctttgag atgcacatag gagtcaacca cttgggtcac ttcctcctaa 
      541 cccatctgct gctagagaaa ctaaaggaat cagccccatc aaggatagta aatgtgtctt 
      601 ccctcgcaca tcacctggga aggatccact tccataacct gcagggcgag aaattctaca 
      661 atgcaggcct ggcctactgt cacagcaagc tagccaacat cctcttcacc caggaactgg 
      721 cccggagact aaaaggctct ggcgttacga cgtattctgt acaccctggc acagtccaat 
      781 ctgaactggt tcggcactca tctttcatga gatggatgtg gtggcttttc tcctttttca 
      841 tcaagactcc tcagcaggga gcccagacca gcctgcactg tgccttaaca gaaggtcttg 
      901 agattctaag tgggaatcat ttcagtgact gtcatgtggc atgggtctct gcccaagctc 
      961 gtaatgagac tatagcaagg cggctgtggg acgtcagttg tgacctgctg ggcctcccaa 
     1021 tagactaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 
     1081 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
     1141 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     1201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     1261 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 
     1321 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
     1381 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
     1441 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
     1501 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 
     1561 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 
     1621 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 
     1681 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 
     1741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     1801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     1861 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 
     1921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 
     1981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     2041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     2101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     2161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     2221 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
     2281 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     2341 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     2401 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 
     2461 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     2521 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     2581 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     2641 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     2701 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     2761 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 
     2821 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     2881 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     2941 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     3001 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
     3061 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
     3121 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     3181 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 
     3241 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 
     3301 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 
     3361 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 
     3421 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 
     3481 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 
     3541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     3601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     3781 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 
     3841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     3901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     3961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     4021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     4081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     4141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     4201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     4261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     4321 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat 
     4381 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 
     4441 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
     4501 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
     4561 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 
     4621 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 
     4681 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
     4741 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 
     4801 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
     4861 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
     4921 taagc 
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