LOCUS       pEGFP-C1-FLAG-BRAT1      7222 bp    DNA     circular     26-AUG-2016
SOURCE      
  ORGANISM  
COMMENT     Created by Sebastien Britton
FEATURES             Location/Qualifiers
     rep_origin      5892..6511
                     /vntifkey="33"
                     /label=pBR322_origin
     terminator      5475..5744
                     /vntifkey="43"
                     /label=TK_PA_terminator
     gene            4509..5297
                     /gene="Neomycin"
                     /vntifkey="60"
     CDS             4506..5300
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="Orf frame 2"
     rep_origin      4322..4399
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        4155..4423
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    complement(4143..4358)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        4043..4071
                     /vntifkey="30"
                     /label=AmpR_promoter
     rep_origin      complement(3658..3964)
                     /vntifkey="33"
                     /label=f1_origin
     terminator      3291..3519
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     gene            1..717
                     /gene="EGFP"
                     /vntifkey="60"
     promoter        7130..7199
                     /vntifkey="30"
                     /label=CMV_promoter
     promoter        6620..7172
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    6675..6962
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    1..726
                     /vntifkey="21"
                     /label=GFP
     misc_feature    772..3237
                     /vntifkey="21"
                     /label=BRAT1
BASE COUNT     1517 a      2146 c      2043 g      1516 t 
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc 
      721 ggactcagat ctgattacaa ggatgacgac gataagctcg agggcggccg catggaccca 
      781 gaatgcgccc agctgctccc ggctctctgt gctgttctgg tagatcccgg gcagccggtg 
      841 gcagatgaca cctgtttgga gaagctcctg gactggttta aaacggtcac tgaaggagag 
      901 tccagtgtcg tgctgctgca ggagcacccc tgcctggtgg agctgctgtc ccatgtgctg 
      961 aaagtccagg acctgagttc tggggtcctc tccttctcac tgcgcctggc aggaaccttc 
     1021 gcagcccagg aaaactgctt ccagtatctt cagcaggggg agttactacc agggctcttt 
     1081 ggggagccag gacccctcgg ccgagcaacc tgggccgtcc ccaccgtgcg cagcggctgg 
     1141 atccagggcc tgcgctccct ggcacagcac cccagcgccc tgcgcttcct ggccgaccat 
     1201 ggtgcggtcg acaccatctt ctccctgcag ggagactcca gcctgtttgt ggcctcggcg 
     1261 gccagtcagc tcctggtgca cgtcctggct ttgtccatgc gaggtggagc cgaggggcag 
     1321 ccctgcctgc cggggggtga ctggcccgcg tgtgcccaga agatcatgga tcacgttgaa 
     1381 gagtccttgt gctccgcggc cacccccaag gtcactcagg ccctgaacgt cctgaccacg 
     1441 accttcgggc gctgccagag cccctggacg gaagccctgt gggtgcggct gagtccccgc 
     1501 gtggcctgtc tgctggagag agaccccatc cccgccgcac actcgttcgt ggacctgctt 
     1561 ctctgtgtgg ctcgttctcc cgtgttcagt tcttccgacg gcagcctgtg ggagacagtg 
     1621 gcgcgggctc tgagctgcct gggtcccacc cacatgggac ccctggcttt ggggatcctg 
     1681 aagctcgagc actgtccaca ggcactgagg acccaggcct tccaggtcct tctccagccc 
     1741 ctggcctgtg tcctgaaggc cacggttcag gcccccggac ccccaggctt gctggacggg 
     1801 acggcagacg atgccacgac ggtggacaca ctcctggcct ccaagtcgtc ctgcgccggc 
     1861 ctcctgtgcc gcaccctggc tcacctggag gagctgcagc cgctgcccca gcgcccttca 
     1921 ccgtggcccc aggcgtctct actgggggct acagtgactg tcctgcggct ctgtgacggc 
     1981 tcggctgccc ctgcctccag tgtggggggc cacctctgtg ggaccctggc gggctgcgtc 
     2041 cgggtccagc gagcagccct cgacttcctg gggacgctgt cacaggggac aggcccccag 
     2101 gagctggtga cgcaggcgct tgctgtcctc ctggagtgcc tcgagagccc cggctccagc 
     2161 cccacggttc tgaagaaggc cttccaggcc acgctcaggt ggctcctgag ctcacccaag 
     2221 acccccggct gctctgatct cggccccctc atcccgcagt tcctcagaga gctgttccct 
     2281 gtgctgcaga aacgcctgtg ccacccctgc tgggaggtga gggactccgc cctcgagttc 
     2341 ctgacccagc tgagcaggca ctggggagga caggctgact tcagatgcgc actcttggct 
     2401 tcagaggtgc ctcagctggc cctgcagctc ctccaggacc ctgagagtta tgtccgagcg 
     2461 agtgcagtga ccgccatggg gcagctgtcc agccagggcc tgcacgcccc caccagccct 
     2521 gagcatgcag aggcccggca gagcctgttc ctggagctcc tgcacatcct ctccgtagac 
     2581 tcggagggct tcccacggcg ggcggtcatg caagtcttca ctgagtggct gcgggacggc 
     2641 cacgccgacg cggcccagga cacggagcag ttcgtggcca ctgtgctgca ggcggcgagc 
     2701 cgagacctgg actgggaggt ccgcgcccag ggcctggagc tggccctcgt gttcctgggc 
     2761 cagactttgg ggccgccgcg tacccactgc ccctatgccg tggccctacc cgaggtggcc 
     2821 ccagcccagc cactcaccga ggcactgagg gctctctgcc acgtggggct ctttgacttc 
     2881 gccttttgtg ccttgtttga ctgcgaccgc cctgtggcgc agaagtcttg tgacctcctt 
     2941 ctcttcctga gggacaagat tgcttcctac agcagcctgc gggaggccag gggcagcccc 
     3001 aacactgcct ccgcagaggc caccctgccg aggtggcggg cgggtgagca ggcccagccc 
     3061 ccaggggacc aggagcctga ggctgtgctg gccatgctca ggtccctaga cctggagggc 
     3121 ctgcggagca cgctggccga gagcagcgac cacgtggaaa agagtcccca gtccctcctg 
     3181 caggacatgc tggccacggg aggcttcctg cagggggacg aggccgactg ctactgagaa 
     3241 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat 
     3301 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 
     3361 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 
     3421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     3481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta 
     3541 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 
     3601 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 
     3661 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 
     3721 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 
     3781 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 
     3841 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 
     3901 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 
     3961 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     4021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     4081 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 
     4141 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     4201 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     4261 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     4321 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     4381 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     4441 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 
     4501 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 
     4561 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 
     4621 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 
     4681 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 
     4741 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 
     4801 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 
     4861 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 
     4921 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 
     4981 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 
     5041 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 
     5101 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 
     5161 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 
     5221 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 
     5281 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 
     5341 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 
     5401 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 
     5461 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 
     5521 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 
     5581 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 
     5641 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 
     5701 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 
     5761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     5821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     6601 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
     6661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
     6721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
     6781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
     6841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
     6901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
     6961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
     7021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
     7081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
     7141 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc 
     7201 gctagcgcta ccggtcgcca cc 
//
