LOCUS       pDY32:_JT39_minCMV      7462 bp ds-DNA     circular     16-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      6869..7457
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2362..2380
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3608..3757
                     /label="PDGFRB Transmembrane Domain"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     misc_feature    2741..2746
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2434..2452
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(6677..6745)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4774..5610
                     /label="right_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        325..342
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4590..4590
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4556..4576
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             1393..1992
                     /label="PuroR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2670..2728
                     /label="minCMV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2797..2816
                     /label="sgRNA_Sp"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3578..3607
                     /label="Myc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3833..3838
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2803..2822
                     /label="sgRNA_Sa"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        343..349
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4751..4758
                     /label="Insertion from Gibson assembly"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2614..2632
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3839..3841
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2849..3529
                     /label="hIgG1-Fc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2326..2344
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             3845..4555
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2398..2416
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     381..397
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    471..1274
                     /label="left_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4591..4591
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     promoter        418..436
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    283..304
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     polyA_signal    4587..4708
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4586..4589
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     promoter        319..324
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4592..4717
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2542..2560
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2309..2632
                     /label="9xTRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    357..373
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             1330..1383
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3842..3844
                     /label="Citrine"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2030..2254
                     /label="polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2578..2596
                     /label="TRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2470..2488
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2506..2524
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1281..1306
                     /label="SA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2747..2809
                     /label="Igκ leader"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    4582..4585
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(5885..6676)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             3773..3826
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3530..3577
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
       61 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      181 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      241 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      301 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      361 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcagaat
      421 taaccctcac taaagggact agtcctgcag gtttaaacga attcgccctt tgctttctct
      481 gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt ggcctgggtc
      541 acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt cttcctccac
      601 tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc cggagcactt
      661 ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg tctgggtcct
      721 ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct gggttccctt
      781 ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct tctccgacgg
      841 atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc gtttttctgg
      901 acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc ttgctttctt
      961 tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt catttgggca
     1021 gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc ctgtcatggc
     1081 atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc ccctatgtcc
     1141 acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct gggaccacct
     1201 tatattccca gggccggtta atgtggctct ggttctgggt acttttatct gtcccctcca
     1261 ccccacagtg gggcaagctt ctgacctctt ctcttcctcc cacagggcct cgagagatct
     1321 ggcagcggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     1381 cctaggctcg agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     1741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     1861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1921 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1981 cccggtgcct gatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2041 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2101 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2161 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2221 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaga accagctggg
     2281 ggctagcaaa gcctaggaac agctgcatat agcactgcag tttactccct atcagtgata
     2341 gagagtcaaa aggccatgcc ctccctatca gtgatagaga gagcagactc aaggtggtcc
     2401 ctatcagtga tagagagatt gagcaagagg gcctccctat cagtgataga gaatagcact
     2461 gcagtttact ccctatcagt gatagagagt caaaaggcca tgccctccct atcagtgata
     2521 gagagagcag actcaaggtg gtccctatca gtgatagaga gattgagcaa gagggcctcc
     2581 ctatcagtga tagagattga tccagtcagg gcctccctat cagtgataga gaagctatgt
     2641 gcaggtaggg ctgtacagtg ggaatgcatG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
     2701 CAGAGCTCGT TTAGTGAACC GTCAGATCcc accgcggtgg gccaccATGG AGACAGACAC
     2761 ACTCCTGCTA TGGGTACTGC TGCTCTGGGT TCCAGGTTCC ACTGGTGACG CTAGCGAGAC
     2821 GGGCGGATCA GGCGGTCGTC TCGGATCCGA CAAAACTCAC ACATGCCCAC CGTGCCCAGC
     2881 ACCTGAACTC CTGGGGGGAC CGTCAGTCTT CCTCTTCCCC CCAAAACCCA AGGACACCCT
     2941 CATGATCTCC CGGACCCCTG AGGTCACATG CGTGGTGGTG GACGTGAGCC ACGAAGACCC
     3001 TGAGGTCAAG TTCAACTGGT ACGTGGACGG CGTGGAGGTG CATAATGCCA AGACAAAGCC
     3061 GCGGGAGGAG CAGTACAACA GCACGTACCG TGTGGTCAGC GTCCTCACCG TCCTGCACCA
     3121 GGACTGGCTG AATGGCAAGG AGTACAAGTG CAAGGTCTCC AACAAAGCCC TCCCAGCCCC
     3181 CATCGAGAAA ACCATCTCCA AAGCCAAAGG GCAGCCCCGA GAACCACAGG TCTACACCCT
     3241 GCCCCCATCC CGGGAGGAGA TGACCAAGAA CCAGGTCAGC CTGACCTGCC TGGTCAAAGG
     3301 CTTCTATCCC AGCGACATCG CCGTGGAGTG GGAGAGCAAT GGGCAGCCGG AGAACAACTA
     3361 CAAGACCACG CCTCCCGTGC TGGACTCCGA CGGCTCCTTC TTCCTCTACA GCAAGCTCAC
     3421 CGTGGACAAG AGCAGGTGGC AGCAGGGGAA CGTCTTCTCA TGCTCCGTGA TGCACGAGGC
     3481 TCTGCACAAC CACTACACGC AGAAGAGCCT CTCCCTGTCT CCGGGTAAAG GAGGAGGTGG
     3541 AAGCGGAGGA GGAGGAAGCG GAGGAGGAGG TAGCggaGAA CAAAAACTCA TCTCAGAAGA
     3601 GGATCTGAAT GCTGTGGGCC AGGACACGCA GGAGGTCATC GTGGTGCCAC ACTCCTTGCC
     3661 CTTTAAGGTG GTGGTGATCT CAGCCATCCT GGCCCTGGTG GTGCTCACCA TCATCTCCCT
     3721 TATCATCCTC ATCATGCTTT GGCAGAAGAA GCCACGTtgt acaGGCAGTG GAGAGGGCAG
     3781 AGGAAGTCTG CTAACATGCG GTGACGTCGA GGAGAATCCT GGCCCAccgg tcgccaccat
     3841 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3901 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     3961 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4021 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     4081 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4141 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4201 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4261 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4321 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4381 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4441 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4501 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagCCAAA
     4561 AAAGAAGAGA AAGGTAtagt taactaaact tgtttattgc agcttataat ggttacaaat
     4621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4681 gtttgtccaa actcatcaat gtatcttatc atgtctgCAt atgtaaattg taagcgtctc
     4741 ggcctctaga GTCGAGATga cattgattat tgaactaggg acaggattgg tgacagaaaa
     4801 gccccatcct taggcctcct ccttcctagt ctcctgatat tgggtctaac ccccacctcc
     4861 tgttaggcag attccttatc tggtgacaca cccccatttc ctggagccat ctctctcctt
     4921 gccagaacct ctaaggtttg cttacgatgg agccagagag gatcctggga gggagagctt
     4981 ggcagggggt gggagggaag ggggggatgc gtgacctgcc cggttctcag tggccaccct
     5041 gcgctaccct ctcccagaac ctgagctgct ctgacgcggc tgtctggtgc gtttcactga
     5101 tcctggtgct gcagcttcct tacacttccc aagaggagaa gcagtttgga aaaacaaaat
     5161 cagaataagt tggtcctgag ttctaacttt ggctcttcac ctttctagtc cccaatttat
     5221 attgttcctc cgtgcgtcag ttttacctgt gagataaggc cagtagccag ccccgtcctg
     5281 gcagggctgt ggtgaggagg ggggtgtccg tgtggaaaac tccctttgtg agaatggtgc
     5341 gtcctaggtg ttcaccaggt cgtggccgcc tctactccct ttctctttct ccatccttct
     5401 ttccttaaag agtccccagt gctatctggg acatattcct ccgcccagag cagggtcccg
     5461 cttccctaag gccctgctct gggcttctgg gtttgagtcc ttggcaagcc caggagaggc
     5521 gctcaggctt ccctgtcccc cttcctcgtc caccatctca tgcccctggc tctcctgccc
     5581 cttccctaca ggggttcctg gctctgctct tcgccttctt gacgagttct tctgaattat
     5641 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5701 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5761 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc
     5821 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5881 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5941 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6001 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6061 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6121 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6181 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6241 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6301 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6361 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6421 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6481 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6541 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6601 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6661 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6721 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6781 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7441 ggcggagcct atggaaaaac gc
//