LOCUS       pJT040:_lenti_MCS_pEF-m 8047 bp ds-DNA     circular     19-DEC-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3965..3995
                     /label="MCS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5205..6164
                     /label="mIFP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7393..7573
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7644..7653)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(350..1009)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3035..3268
                     /label="RRE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2184..2364
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6266..6866
                     /label="wPRE"
                     /ApEinfo_revcolor=#0060ff
                     /ApEinfo_fwdcolor=#0060ff
     misc_feature    4986..5140
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_recomb     6187..6220
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     rep_origin      complement(join(7617..8047,1..252))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_fwdcolor=#808080
     misc_feature    1603..2166
                     /label="pU6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4023..4984
                     /label="EF1Alpha"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2106..2115)
                     /label="GC Box"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7056..7070
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2941..2947)
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3799..3813
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1249..1277)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(join(7620..8047,1..192))
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
       61 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      121 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      181 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      241 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      301 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      361 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      421 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      481 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      541 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      601 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      661 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      721 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      781 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      841 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      901 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      961 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1021 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1081 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1141 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1201 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1261 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1321 cccgaaaagt gccacctgac gcttaagcgg tcgacggatc gggagatctc ccgatcccct
     1381 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1441 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1501 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1561 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     1621 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1681 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1741 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1801 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1861 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1921 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1981 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2041 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2101 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2161 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2341 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2401 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2461 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2521 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2581 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2641 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2701 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     2761 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     2821 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     2881 ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2941 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3001 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3061 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3121 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3181 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3241 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3301 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3361 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3421 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3481 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3541 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3601 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3661 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     3721 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     3781 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     3841 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     3901 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc
     3961 ccgctctaga GATCCCATTT GACTCAGgcg gccgcgtgga taaccgtatt accgccatgc
     4021 atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG
     4081 GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT
     4141 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT
     4201 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG
     4261 TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC
     4321 CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG
     4381 TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG
     4441 GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA
     4501 TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA
     4561 TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG
     4621 CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG
     4681 GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT
     4741 CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC
     4801 GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG
     4861 GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC
     4921 AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT
     4981 CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT
     5041 TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA TGTAATTCTC
     5101 CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
     5161 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAGC CACCATGTCG GTACCGCTGA
     5221 CTACCTCAGC ATTCGGCCAC GCGTTTCTGG CTAACTGTGA ACGCGAGCAG ATCCACCTGG
     5281 CGGGCTCCAT TCAGCCGCAC GGTATCCTGC TGGCTGTGAA AGAGCCGGAC AACGTGGTGA
     5341 TCCAGGCTTC TATTAACGCT GCGGAGTTCC TGAACACCAA CTCTGTTGTT GGCCGTCCGC
     5401 TGCGTGACCT GGGCGGCGAT CTGCCTTTGC AGATCCTGCC GCACCTGAAC GGCCCGCTGC
     5461 ACCTGGCTCC GATGACCCTG CGTTGTACCG TGGGTTCTCC GCCGCGTCGT GTGGACTGTA
     5521 CCATTCATCG TCCGTCTAAC GGCGGCCTGA TCGTAGAACT GGAACCAGCA ACCAAGACCA
     5581 CTAACATTGC GCCGGCTCTG GACGGTGCGT TTCATCGTAT CACTTCTTCA TCCTCCCTGA
     5641 TGGGCCTGTG TGACGAAACC GCGACTATTA TCCGTGAGAT TACTGGCTAC GACCGTGTGA
     5701 TGGTAGTACG TTTCGATGAA GAGGGTAATG GCGAAATTCT GTCCGAACGT CGTCGTGCGG
     5761 ACCTGGAAGC GTTCCTGGGT AACCGCTACC CGGCGTCTAC TATTCCGCAG ATCGCTCGTC
     5821 GCCTGTACGA ACATAACCGT GTTCGCCTGC TGGTAGATGT GAACTATACT CCGGTTCCGC
     5881 TACAGCCGCG CATCAGCCCG CTGAACGGTC GTGATCTGGA TATGTCCCTG TCTTGCCTGC
     5941 GCTCTATGTC CCCGATCCAC CAGAAATACA TGCAGGACAT GGGCGTTGGC GCGACCCTGG
     6001 TTTGCTCTCT GATGGTGTCT GGTCGTCTGT GGGGTCTGAT CGCTTGCCAC CACTACGAAC
     6061 CGCGCTTCGT TCCGTTCCAC ATTCGCGCTG CTGGCGAAGC GCTGGCGGAA ACTTGTGCGA
     6121 TCCGCATCGC GACGCTGGAG AGCTTTGCAC AGTCTCAGTC CAAATAGgaa ttcgtcgagg
     6181 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
     6241 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     6301 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6361 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6421 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6481 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6541 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6601 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6661 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6721 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6781 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6841 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     6901 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     6961 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7021 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7081 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7141 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     7201 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     7261 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     7321 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     7381 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7441 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7501 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7561 aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8041 ttgaagt
//