LOCUS       IBMc318                 2846 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(2025..2044)
                     /label="VR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1192..1935
                     /label="ER-LBD"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1040..1059
                     /label="VF2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(59..919)
                     /label="ampR (rev)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1158..1179
                     /label="RFC10 Prefix (full)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1947..1967
                     /label="RFC10 Suffix"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(2131..2745)
                     /label="rep (pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1169..1190
                     /label="IBMo0681"
                     /note="sequence: CGCTTCTAGAGGAAGACGCTGG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(1166..1191)
                     /label="IBMo0643"
                     /note="sequence: TGATCATCGTCTCATCCAGCGTCTTCCTCTAGAAGCGGCC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1936..1961
                     /label="IBMo0642"
                     /note="sequence: TGATCATCGTCTCTCAAGAAGAGCtactagtagcggccg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2801..2834
                     /label="IBMo0535"
                     /note="sequence: ggtcatgagattatcaaaaaggatcttcacctag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1825..1855
                     /label="IBMo0757"
                     /note="sequence: CGTCATATGAGCAATAAAGGTATGGAACACC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1937..1959)
                     /label="IBMo0682"
                     /note="sequence: GCCGCTACTAGTAGCTCTTCTTG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2801..2834)
                     /label="IBMo0534"
                     /note="sequence: ctaggtgaagatcctttttgataatctcatgacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
       61 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      181 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      361 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      661 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      781 tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1021 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1081 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     1141 gctaaggatg atttctggaa ttcgcggccg cttctagagG AAGACGCTGG AAATAGCCTG
     1201 GCACTGAGCC TGACCGCAGA TCAGATGGTT AGCGCACTGC TGGATGCAGA ACCGCCTATT
     1261 CTGTATAGCG AATATGATCC GACACGTCCG TTTAGCGAAG CAAGCATGAT GGGTCTGCTG
     1321 ACCAATCTGG CAGATCGTGA ACTGGTTCAT ATGATTAATT GGGCAAAACG TGTTCCGGGT
     1381 TTTGTTGATC TGACCCTGCA TGATCAGGTT CATCTGCTGG AATGTGCATG GCTGGAAATT
     1441 CTGATGATTG GTCTGGTTTG GCGTAGCATG GAACATCCGG GTAAACTGCT GTTTGCACCG
     1501 AATCTGCTGC TGGATCGTAA TCAGGGTAAA TGTGTTGAAG GTATGGTGGA AATCTTTGAT
     1561 ATGCTGCTGG CAACCAGCAG CCGTTTTCGT ATGATGAATC TGCAAGGTGA AGAATTTGTT
     1621 TGCCTGAAAA GCATTATTCT GCTGAACAGC GGTGTTTATA CCTTTCTGAG CAGCACCCTG
     1681 AAAAGTCTGG AAGAAAAAGA TCATATCCAT CGTGTGCTGG ATAAAATCAC CGATACACTG
     1741 ATTCATCTGA TGGCAAAAGC CGGTCTGACC TTACAGCAGC AGCATCAGCG TCTGGCACAA
     1801 CTGCTGCTGA TTCTGAGCCA TATTCGTCAT ATGAGCAATA AAGGTATGGA ACACCTGTAC
     1861 AGCATGAAAT GCAAAAATGT TGTTCCGCTG TATGACCTGC TGTTAGAAAT GCTGGACGCA
     1921 CATCGTCTGC ATGCATCAAG AAGAGCtact agtagcggcc gctgcaggct tcctcgctca
     1981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     2161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2821 ggatcttcac ctagatcctt ttaaat
//