LOCUS       IBMc097                 2660 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
KEYWORDS    "Index:IBMc097" "Record:CLONE029"                                  
FEATURES             Location/Qualifiers
     rep_origin      complement(1880..2494)
                     /label="rep (pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1030..1035
                     /label="BsaI site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1620..1640
                     /label="RFC10 Suffix"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     890..909
                     /label="VF2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1614..1619)
                     /label="BsaI site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(119..778)
                     /label="cat (BsmBI-)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1008..1029
                     /label="RFC10 Prefix (full)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             1037..1612
                     /label="mCherry*"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1609..1612
                     /label="BsaI overhang"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(1774..1793)
                     /label="VR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1037..1040
                     /label="BsaI overhang"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1008..1029)
                     /label="BBa_G1001"
                     /note="sequence: ctctagaagcggccgcgaattc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1620..1640
                     /label="BBa_G1000"
                     /note="sequence: tactagtagcggccgctgcag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1008..1057
                     /label="IBMo0216"
                     /note="sequence: gaattcgcggccgcttctagagGGTCTCAgtgaacggccacgagttcgag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1588..1641)
                     /label="IBMo0217"
                     /note="sequence: actgcagcggccgctactagtaGGTCTCActgttccacgatggtgtagtcctcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
       61 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
      121 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      181 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      241 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      301 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaAACgaaa aacatattct
      361 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
      421 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
      481 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
      541 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
      601 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      661 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      721 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
      781 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
      841 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
      901 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
      961 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     1021 cttctagagG GTCTCAgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1081 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1141 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1201 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1261 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg
     1321 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1381 gcagaagAAA accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1441 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1501 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1561 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agTGAGACCt
     1621 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
     1681 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     1741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1861 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2641 tggtctgaca gctcgaggct
//