LOCUS       IBMc367                 2633 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1694..1714
                     /label="RFC10 Suffix"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1688..1693)
                     /label="BsaI site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1848..1867)
                     /label="VR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1110..1131
                     /label="RFC10 Prefix (full)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1139..1142
                     /label="IBM BsaI overhang"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1132..1137
                     /label="BsaI site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1683..1686
                     /label="IBM BsaI overhang"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1139..1685
                     /label="ECF20"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1954..2568)
                     /label="rep (pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     992..1011
                     /label="VF2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(221..880)
                     /label="cat (BsmBI-)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1666..1707)
                     /label="IBMo0761"
                     /note="sequence: ggccgctactagtaGGTCTCAcagatctgcacccggtgcttc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1118..1168
                     /label="IBMo0760"
                     /note="sequence: ggccgcttctagagGGTCTCAatcctgatctggaactgctgaaacgtattg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
       61 taaagtatat atgagtaaac ttggtctcga cagctcgagg cttggattct caccaataaa
      121 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      181 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      241 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      301 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      361 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      421 tgaaactcac ccagggattg gctgaAACga aaaacatatt ctcaataaac cctttaggga
      481 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      541 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      601 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      661 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      721 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      781 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      841 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      901 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      961 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     1021 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     1081 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGGTCTCAat
     1141 cctgatctgg aactgctgaa acgtattggt aataatgatg cacaggccgt taaagaaatg
     1201 gttacccgta aactgcctcg tctgctggca ctggcaagtc gcctgctggg tgatgcagat
     1261 gaagcacgtg atattgcaca agaaagtttt ctgcgcattt ggaaacaggc agcaagctgg
     1321 cgtagcgaac aggcacgttt tgatacctgg ctgcatcgtg ttgcactgaa tctgtgttat
     1381 gatcgtctgc gtcgtcgtaa agaacatgtg ccggttgata gcgaacatgc ctgtgaagca
     1441 ctggataccc gtccggcacc ggatgaacag ctggaagcaa gcgcacagag ccgtcgtatg
     1501 gcacaggcac tggatcagct gccggatcgt cagcgtgaag caattgttct gcagtattat
     1561 caagaactga gcaataccga agcagcagca ctgatgcaaa ttagcgttga agccctggaa
     1621 agcctgctga gccgtgcacg tcgtaatctg cgtagccatc tggccgaagc accgggtgca
     1681 gatctgTGAG ACCtactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac
     1741 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
     1801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     1981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2401 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aga
//