LOCUS       IBMc326                 4607 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2785..2788
                     /label="IBM BsaI overhang"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     RBS             2714..2724
                     /label="BBa_B0033"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1494..1515
                     /label="RFC10 Prefix (full)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             2785..2809
                     /label="ECF20"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1516..2394)
                     /label="araC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             98..1291
                     /label="tetR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(2986..3005)
                     /label="VR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      3452..4276
                     /label="p15A origin of replication"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        2422..2705
                     /label="PBAD(SapI-)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        9..97
                     /label="tetR promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      2818..2870
                     /label="ECK120033736"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2744..2747
                     /label="IBM BsaI overhang"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2778..2783
                     /label="BsaI recognition site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2871..2891
                     /label="RFC10 Suffix"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1371..1390
                     /label="VF2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             2731..2747
                     /label="ECF20"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2749..2754)
                     /label="BsaI recognition site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt
       61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
      121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact
      181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct
      241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga
      301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc
      361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat
      421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga
      481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt
      601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga
      661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg
      721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt
      781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
      841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg
      901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc
      961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat
     1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt
     1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc
     1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg
     1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg
     1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga
     1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag
     1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg
     1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat
     2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga gaccCTGTCT
     2761 CTTATACACA TCTATTCggt ctcATctgag cggtcgtcgc aaaccgtaat actagagaac
     2821 gcatgagAAA GCCCCCGGAA GATCACCTTC CGGGGGCTTT tttattgcgc tactagtagc
     2881 ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc aaaagcctcc
     2941 gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca ctcaaaggcg
     3001 gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta gagatggaat
     3061 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3121 ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta tcattgcagc
     3181 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3241 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3301 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3421 tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt gagatcgttt
     3481 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     3541 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
     3601 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     3661 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     3721 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     3781 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     3841 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     3901 agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     3961 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     4021 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     4081 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     4141 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     4201 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     4261 agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
     4321 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
     4381 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     4441 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
     4501 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     4561 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtt
//