LOCUS       IBMc259                 4024 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             186..977
                     /label="SmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      3794..3854
                     /label="L3S2P21"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1242..2192)
                     /label="Rep101"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     terminator      complement(2729..2815)
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3855..3875
                     /label="RFC10 Suffix"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(3934..3950)
                     /label="pSEVA PS2 Rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     insulator       2994..3068
                     /label="RiboJ10"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3095..3790
                     /label="mScarlet-I"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2719..2736
                     /label="pSEVA PS1 Fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2918..2993
                     /label="pTet*"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3880..3974
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             3077..3088
                     /label="BBa_B0064"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      2240..2462
                     /label="pSC101 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2877..2898
                     /label="RFC10 Prefix (full)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
       61 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      121 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      181 acatcATGAG GGAAGCGGTG ATCGCCGAAG TATCGACTCA ACTATCAGAG GTAGTTGGCG
      241 TCATCGAGCG CCATCTCGAA CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG
      301 ATGGCGGCCT GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG
      361 ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT TCCCCTGGAG
      421 AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT GCACGACGAC ATCATTCCGT
      481 GGCGTTATCC AGCTAAGCGC GAACTGCAAT TTGGAGAATG GCAGCGCAAT GACATTCTTG
      541 CAGGTATCTT CGAGCCAGCC ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA
      601 GAGAACATAG CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG
      661 AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG CCGCCCGACT
      721 GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG CATTTGGTAC AGCGCAGTAA
      781 CCGGCAAAAT CGCGCCGAAG GATGTCGCTG CCGACTGGGC AATGGAGCGC CTGCCGGCCC
      841 AGTATCAGCC CGTCATACTT GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT
      901 TGGCCTCGCG CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA
      961 AGGTAGTCGG CAAATAAGAC aaTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT
     1021 TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG
     1081 GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT
     1141 AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT
     1201 CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CTCAGATCCT TCCGTATTTA
     1261 GCCAGTATGT TCTCTAGTGT GGTTCGTTGT TTTTGCGTGA GCCATGAGAA CGAACCATTG
     1321 AGATCATACT TACTTTGCAT GTCACTCAAA AATTTTGCCT CAAAACTGGT GAGCTGAATT
     1381 TTTGCAGTTA AAGCATCGTG TAGTGTTTTT CTTAGTCCGT TATGTAGGTA GGAATCTGAT
     1441 GTAATGGTTG TTGGTATTTT GTCACCATTC ATTTTTATCT GGTTGTTCTC AAGTTCGGTT
     1501 ACGAGATCCA TTTGTCTATC TAGTTCAACT TGGAAAATCA ACGTATCAGT CGGGCGGCCT
     1561 CGCTTATCAA CCACCAATTT CATATTGCTG TAAGTGTTTA AATCTTTACT TATTGGTTTC
     1621 AAAACCCATT GGTTAAGCCT TTTAAACTCA TGGTAGTTAT TTTCAAGCAT TAACATGAAC
     1681 TTAAATTCAT CAAGGCTAAT CTCTATATTT GCCTTGTGAG TTTTCTTTTG TGTTAGTTCT
     1741 TTTAATAACC ACTCATAAAT CCTCATAGAG TATTTGTTTT CAAAAGACTT AACATGTTCC
     1801 AGATTATATT TTATGAATTT TTTTAACTGG AAAAGATAAG GCAATATCTC TTCACTAAAA
     1861 ACTAATTCTA ATTTTTCGCT TGAGAACTTG GCATAGTTTG TCCACTGGAA AATCTCAAAG
     1921 CCTTTAACCA AAGGATTCCT GATTTCCACA GTTCTCGTCA TCAGCTCTCT GGTTGCTTTA
     1981 GCTAATACAC CATAAGCATT TTCCCTACTG ATGTTCATCA TCTGAGCGTA TTGGTTATAA
     2041 GTGAACGATA CCGTCCGTTC TTTCCTTGTA GGGTTTTCAA TCGTGGGGTT GAGTAGTGCC
     2101 ACACAGCATA AAATTAGCTT GGTTTCATGC TCCGTTAAGT CATAGCGACT AATCGCTAGT
     2161 TCATTTGCTT TGAAAACAAC TAATTCAGAC ATACATCTCA ATTGGTCTAG GTGATTTTAA
     2221 TCACTATACC AATTGAGATG GGCTAGTCAA TGATAATTAC ATGTCCTTTT CCTTTGAGTT
     2281 GTGGGTATCT GTAAATTCTG CTAGACCTTT GCTGGAAAAC TTGTAAATTC TGCTAGACCC
     2341 TCTGTAAATT CCGCTAGACC TTTGTGTGTT TTTTTTGTTT ATATTCAAGT GGTTATAATT
     2401 TATAGAATAA AGAAAGAATA AAAAAAGATA AAAAGAATAG ATCCCAGCCC TGTGTATAAC
     2461 TCACTACTTT AGTCAGTTCC GCAGTATTAC AAAAGGATGT CGCAAACGCT GTTTGCTCCT
     2521 CTACAAAACA GACCTTAAAA CCCTAAAGGC TTAAGTAGCA CCCTCGCAAG CTCGGGCAAA
     2581 TCGCTGAATA TTCCTTTTGT CTCCGACCAT CAGGCACCTG AGTCGCTGTC TTTTTCGTGA
     2641 CATTCAGTTC GCTGCGCTCA CGGCTCTGGC AGTGAATGGG GGTAAATGGC ACTACAGGCG
     2701 CGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA
     2761 AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC
     2821 TTTAATTAAA GCGGATAACA ATTTCACACA GGAGGCCGCC TAGGCCGCGG CCGCGCGaat
     2881 tcgcggccgc ttctagaggc ttaacgatcg ttggctgttt tcagcaggac gcactgacct
     2941 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacagcgctc
     3001 aacgggtgtg cttcccgttc tgatgagtcc gtgaggacga aagcgcctct acaaataatt
     3061 ttgtttaata ctagagaaag aggggaaata ctagatggtg agtaaaggag aagctgtgat
     3121 taaagagttc atgcgcttca aagttcacat ggagggttct atgaacggtc acgagttcga
     3181 gatcgaaggc gaaggcgagg gccgtccgta tgaaggcacc cagaccgcca aactgaaagt
     3241 gactaaaggc ggcccgctgc ctttttcctg ggacatcctg agcccgcaat ttatgtacgg
     3301 ttctagggcg ttcatcaaac acccagcgga tatcccggac tattataagc agtcttttcc
     3361 ggaaggtttc aagtgggaac gcgtaatgaa ttttgaagat ggtggtgccg tgaccgtcac
     3421 tcaggacacc tccctggagg atggcaccct gatctataaa gttaaactgc gtggtactaa
     3481 ttttccacct gatggcccgg tgatgcagaa aaagacgatg ggttgggagg cgtctaccga
     3541 acgcttgtat ccggaagatg gtgtgctgaa aggcgacatt aaaatggccc tgcgcctgaa
     3601 agatggcggc cgctatctgg ctgacttcaa aaccacgtac aaagccaaga aacctgtgca
     3661 gatgcctggc gcgtacaatg tggaccgcaa actggacatc acctctcata atgaagatta
     3721 tacggtggta gagcaatatg agcgctccga gggtcgtcat tctaccggtg gcatggatga
     3781 actatacaaa taactcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt
     3841 tttcgttttg gtcctactag tagcggccgc tgcagCTTGG ACTCCTGTTG ATAGATCCAG
     3901 TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC CGGGCGTTTT
     3961 TTATTGGTGA GAATCCAGGG GTCCCCAATA ATTACGATTT ACGTATTTAA ATgaaccttg
     4021 accg
//