LOCUS       IBMc330                 3991 bp ds-DNA     circular     09-OCT-2020
DEFINITION  .
KEYWORDS    "creator:Prabu" "marker:AmpR"                                      
FEATURES             Location/Qualifiers
     promoter        1383..1474
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        319..385
                     /label="PSrpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1117..1173
                     /label="L3S2P11"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(2644..3594)
                     /label="Rep101"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     primer_bind     130..147
                     /label="pSEVA PS1 Fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(1262..1278)
                     /label="pSEVA PS2 Rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             394..408
                     /label="BBa_B0030"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             415..1113
                     /label="2ndVal-mScarlet-I"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1475..2335
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1208..1302
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2339..2378
                     /label="fd terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      3642..3864
                     /label="pSC101 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(140..226)
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1085..1133)
                     /label="IBMo0724"
                     /note="sequence: TGGAATTTGGTACCGAGttattatttgtatagttcatccatgccaccgg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1114..1139
                     /label="oTCI0050"
                     /note="sequence: TAACTCGGTACCAAATTCCAGAAAAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(376..414)
                     /label="IBMo0723"
                     /note="sequence: CTAGTATTTCTCCTCTTTAATCTCTAGTAGTTTACAAAC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          395..437
                     /label="IBMo0256"
                     /note="sequence: TTAAAGAGGAGAAATACTAGatggtgagtaaaggagaagctgt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ATTCCTTTTG TCTCCGACCA TCAGGCACCT GAGTCGCTGT CTTTTTCGTG ACATTCAGTT
       61 CGCTGCGCTC ACGGCTCTGG CAGTGAATGG GGGTAAATGG CACTACAGGC GCGGCGCGCC
      121 CAGCTGTCTA GGGCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA
      181 TAAAACGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTTTAATTAA
      241 AGCGGATAAC AATTTCACAC AGGAGGCCGC CTAGGCCGCG GCCGCGCGAA TTCcttctag
      301 agGAAGACag tacgagagga ttcgttacca attgacagct agctcagtcc taggtATATA
      361 CATACATGCT TGTTTGTTTG TAAACTACTA GAGATTAAAG AGGAGAAATA CTAGatggtg
      421 agtaaaggag aagctgtgat taaagagttc atgcgcttca aagttcacat ggagggttct
      481 atgaacggtc acgagttcga gatcgaaggc gaaggcgagg gccgtccgta tgaaggcacc
      541 cagaccgcca aactgaaagt gactaaaggc ggcccgctgc ctttttcctg ggacatcctg
      601 agcccgcaat ttatgtacgg ttctagggcg ttcatcaaac acccagcgga tatcccggac
      661 tattataagc agtcttttcc ggaaggtttc aagtgggaac gcgtaatgaa ttttgaagat
      721 ggtggtgccg tgaccgtcac tcaggacacc tccctggagg atggcaccct gatctataaa
      781 gttaaactgc gtggtactaa ttttccacct gatggcccgg tgatgcagaa aaagacgatg
      841 ggttgggagg cgtctaccga acgcttgtat ccggaagatg gtgtgctgaa aggcgacatt
      901 aaaatggccc tgcgcctgaa agatggcggc cgctatctgg ctgacttcaa aaccacgtac
      961 aaagccaaga aacctgtgca gatgcctggc gcgtacaatg tggaccgcaa actggacatc
     1021 acctctcata atgaagatta tacggtggta gagcaatatg agcgctccga gggtcgtcat
     1081 tctaccggtg gcatggatga actatacaaa taataaCTCG GTACCAAATT CCAGAAAAGA
     1141 GACGCTTTCG AGCGTCTTTT TTCGTTTTGG TCCgtacagc tgcGTCTTCt actagtaCTG
     1201 CAGCTTGGAC TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC
     1261 AGAACGCTCG GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAGGGGT CCCCAATAAT
     1321 TACGATTTAC GTATTTaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
     1381 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1441 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
     1501 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
     1561 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
     1621 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
     1681 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
     1741 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
     1801 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
     1861 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
     1921 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
     1981 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     2041 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     2101 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     2161 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     2221 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     2281 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     2341 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccTTGTCC TTTTCCGCTG
     2401 CATAACCCTG CTTCGGGGTC ATTATAGCGA TTTTTTCGGT ATATCCATCC TTTTTCGCAC
     2461 GATATACAGG ATTTTGCCAA AGGGTTCGTG TAGACTTTCC TTGGTGTATC CAACGGCGTC
     2521 AGCCGGGCAG GATAGGTGAA GTAGGCCCAC CCGCGAGCGG GTGTTCCTTC TTCACTGTCC
     2581 CTTATTCGCA CCTGGCGGTG CTCAACGGGA ATCCTGCTCT GCGAGGCTGG CCGTAGGCCG
     2641 GCCTCAGATC CTTCCGTATT TAGCCAGTAT GTTCTCTAGT GTGGTTCGTT GTTTTTGCGT
     2701 GAGCCATGAG AACGAACCAT TGAGATCATA CTTACTTTGC ATGTCACTCA AAAATTTTGC
     2761 CTCAAAACTG GTGAGCTGAA TTTTTGCAGT TAAAGCATCG TGTAGTGTTT TTCTTAGTCC
     2821 GTTATGTAGG TAGGAATCTG ATGTAATGGT TGTTGGTATT TTGTCACCAT TCATTTTTAT
     2881 CTGGTTGTTC TCAAGTTCGG TTACGAGATC CATTTGTCTA TCTAGTTCAA CTTGGAAAAT
     2941 CAACGTATCA GTCGGGCGGC CTCGCTTATC AACCACCAAT TTCATATTGC TGTAAGTGTT
     3001 TAAATCTTTA CTTATTGGTT TCAAAACCCA TTGGTTAAGC CTTTTAAACT CATGGTAGTT
     3061 ATTTTCAAGC ATTAACATGA ACTTAAATTC ATCAAGGCTA ATCTCTATAT TTGCCTTGTG
     3121 AGTTTTCTTT TGTGTTAGTT CTTTTAATAA CCACTCATAA ATCCTCATAG AGTATTTGTT
     3181 TTCAAAAGAC TTAACATGTT CCAGATTATA TTTTATGAAT TTTTTTAACT GGAAAAGATA
     3241 AGGCAATATC TCTTCACTAA AAACTAATTC TAATTTTTCG CTTGAGAACT TGGCATAGTT
     3301 TGTCCACTGG AAAATCTCAA AGCCTTTAAC CAAAGGATTC CTGATTTCCA CAGTTCTCGT
     3361 CATCAGCTCT CTGGTTGCTT TAGCTAATAC ACCATAAGCA TTTTCCCTAC TGATGTTCAT
     3421 CATCTGAGCG TATTGGTTAT AAGTGAACGA TACCGTCCGT TCTTTCCTTG TAGGGTTTTC
     3481 AATCGTGGGG TTGAGTAGTG CCACACAGCA TAAAATTAGC TTGGTTTCAT GCTCCGTTAA
     3541 GTCATAGCGA CTAATCGCTA GTTCATTTGC TTTGAAAACA ACTAATTCAG ACATACATCT
     3601 CAATTGGTCT AGGTGATTTT AATCACTATA CCAATTGAGA TGGGCTAGTC AATGATAATT
     3661 ACATGTCCTT TTCCTTTGAG TTGTGGGTAT CTGTAAATTC TGCTAGACCT TTGCTGGAAA
     3721 ACTTGTAAAT TCTGCTAGAC CCTCTGTAAA TTCCGCTAGA CCTTTGTGTG TTTTTTTTGT
     3781 TTATATTCAA GTGGTTATAA TTTATAGAAT AAAGAAAGAA TAAAAAAAGA TAAAAAGAAT
     3841 AGATCCCAGC CCTGTGTATA ACTCACTACT TTAGTCAGTT CCGCAGTATT ACAAAAGGAT
     3901 GTCGCAAACG CTGTTTGCTC CTCTACAAAA CAGACCTTAA AACCCTAAAG GCTTAAGTAG
     3961 CACCCTCGCA AGCTCGGGCA AATCGCTGAA T
//