LOCUS       pGEM-T-egfp-aid         3887 bp ds-DNA     circular SYN 18-OCT-2019
DEFINITION  Parental vector for TA cloning of PCR products. The insertion site
            is flanked by BstZI sites. This vector is also known as
            pGEM(R)-5Zf(+).
ACCESSION
VERSION     .
KEYWORDS    pGEM-T.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2015 from SnapGene 2.8.3 http://www.snapgene.com
COMMENT     Linearize at EcoRV to create a TA cloning vector.
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    10..1000
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    32..>42
                     /standard_name="pgemt-gibson-farm"
     primer_bind     52..71
                     /standard_name="52 F"
     misc_feature    54..940
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    777..908
                     /standard_name="nanoaid"
     primer_bind     complement(919..938)
                     /standard_name="938 R"
     misc_feature    complement(<948..958)
                     /standard_name="pgemt-gibson-rarm"
     promoter        complement(1012..1030)
     primer_bind     complement(1048..1064)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1072..1088
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1096..1126)
     rep_origin      complement(1450..2038)
     CDS             complement(2209..3069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(3070..3174)
     rep_origin      complement(3252..3707)
     CDS             complement(3684..1052)
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
     primer_bind     3848..3864
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3871..2
ORIGIN      
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcatcga cttcaaggag gacggaacat cctggggcac aagctggagt
      481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc agtggaccta
      781 aagatccagc caaacctccg gccaaggcac aagttgtggg atggccaccg gtgagatcat
      841 accggaagaa cgtgatggtt tcctgccaaa aatcaagcgg tggcccggag gcggcggcgt
      901 tcgtgaaggg aggaggtggg tctggaggtg gaggatctat cactagtgcg gccgcctgca
      961 ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
     1021 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3181 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3241 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3301 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3361 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3421 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3481 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3541 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3601 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3661 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     3721 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3781 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3841 cgacgttgta aaacgacggc cagtgaattg taatacgact cactata
//
