LOCUS       GAlinker-HA-halo-HA-gp2a-neoR        1815 bp ds-DNA     linear       13-NOV-2019
DEFINITION  .
ACCESSION   urn.local...33-74p5jkc
VERSION     urn.local...33-74p5jkc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..1813
                     /standard_name="ATGAGTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATG
                     GAAGGCAACGTAAACGGGCACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCCTT
                     TTGAGGGAAAACAGAGTATGGATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGC
                     CTTTGATATCCTGACCACTGCATTCTCGTACGGCAACAGGGTATTCGCCAAATATCCA
                     GACAACATACAAGACTATTTTAAGCAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAA
                     GCTTGACTTTCGAAGACGGGGGCATTTGCAACGCCAGAAACGACATAACAATGGAAGG
                     GGACACTTTCTATAATAAAGTTCGATTTTATGGTACCAACTTTCCCGCCAATGGTCCA
                     GTTATGCAGAAGAAGACGCTGAAATGGGAGCCCTCCACTGAGAAAATGTATGTGCGTG
                     ATGGAGTGCTGACGGGTGATATTGAGATGGCTTTGTTGCTTGAAGGAAATGCCCATTA
                     CCGATGTGACTTCAGAACTACTTACAAAGCTAAGGAGAAGGGTGTCAAGTTACCAGGC
                     GCCCACTTTGTGGACCACTGCATTGAGATTTTAAGCCATGACAAAGATTACAACAAGG
                     TTAAGCTGTATGAGCATGCTGTTGCTCATTCTGGATTGCCTGACAATGCCAGAC"
     misc_feature    1..15
                     /standard_name="GA linker"
     primer_bind     complement(8..27)
                     /standard_name="27 R"
     misc_feature    16..42
                     /standard_name="HA"
     primer_bind     43..63
                     /standard_name="1 F"
     primer_bind     43..62
                     /standard_name="1 F"
     primer_bind     43..61
                     /standard_name="1 F"
     misc_feature    216..235
                     /standard_name="validation primer_halo"
     primer_bind     complement(216..235)
                     /standard_name="235 R"
     misc_feature    complement(904..924)
                     /standard_name="882 R"
     misc_feature    complement(905..924)
                     /standard_name="882 R"
     misc_feature    925..951
                     /standard_name="HA"
     primer_bind     complement(931..951)
                     /standard_name="951 R"
     misc_feature    952..960
                     /standard_name="P2A"
     misc_feature    952..960
                     /standard_name="Digestion of pSLQ1501_Actual_ (modified)
                     -Fragment 3 (concatenated sequence 1)"
     misc_feature    952..960
                     /standard_name="GSG linker"
     primer_bind     961..979
                     /standard_name="751 F"
     misc_feature    961..1017
                     /standard_name="Digestion of pSLQ1501_Actual_ (modified)
                     -Fragment 3 (concatenated sequence 1)"
     misc_feature    961..1017
                     /standard_name="P2A"
     misc_feature    complement(999..1017)
                     /standard_name="744 R"
     source          1018..1815
     primer_bind     1018..1038
                     /standard_name="745 F"
     CDS             1024..1815
                     /standard_name="NeoR"
     misc_feature    1611..1630
                     /standard_name="internal validation primer_neoR"
     misc_feature    complement(1794..1815)
                     /standard_name="1,779 R"
     misc_feature    complement(1795..1815)
                     /standard_name="1,542 R"
     misc_feature    complement(1796..1815)
                     /standard_name="1,542 R"
     misc_feature    complement(1797..1815)
                     /standard_name="1,542 R"
ORIGIN      
        1 gctggagccg gtgcctaccc atatgacgtg ccagactacg ccgaaatcgg tactggcttt
       61 ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt
      121 ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg
      181 cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt
      241 atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg
      301 gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc
      361 tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt
      421 atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc
      481 ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt
      541 atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat
      601 taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag
      661 ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg
      721 ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca
      781 ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc
      841 ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc
      901 tggctgtcta ctctggagat ttcctaccca tatgacgtgc cagactacgc cggaagcgga
      961 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctaag
     1021 cttattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1081 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     1141 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1201 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1261 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1321 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1381 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1441 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1501 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1561 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1621 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1681 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     1741 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1801 gacgagttct tctga
//
