LOCUS       puroR-Gp2a-HA-ha        1611 bp    DNA     linear   UNA 25-APR-2017
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...e-7a24iff
VERSION     urn.local...e-7a24iff
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..600
                     /note="Geneious type: Marker"
                     /gene="PuroR marker"
     primer_bind     1..20
                     /standard_name="988 F"
     primer_bind     1..19
                     /standard_name="1 F"
     primer_bind     1..20
                     /standard_name="1 F"
     misc_feature    2..1611
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATGGAAG
                     GCAACGTAAACGGGCACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCCTTTTGA
                     GGGAAAACAGAGTATGGATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGCCTTT
                     GATATCCTGACCACTGCATTCTCGTACGGCAACAGGGTATTCGCCAAATATCCAGACA
                     ACATACAAGACTATTTTAAGCAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAAGCTT
                     GACTTTCGAAGACGGGGGCATTTGCAACGCCAGAAACGACATAACAATGGAAGGGGAC
                     ACTTTCTATAATAAAGTTCGATTTTATGGTACCAACTTTCCCGCCAATGGTCCAGTTA
                     TGCAGAAGAAGACGCTGAAATGGGAGCCCTCCACTGAGAAAATGTATGTGCGTGATGG
                     AGTGCTGACGGGTGATATTGAGATGGCTTTGTTGCTTGAAGGAAATGCCCATTACCGA
                     TGTGACTTCAGAACTACTTACAAAGCTAAGGAGAAGGGTGTCAAGTTACCAGGCGCCC
                     ACTTTGTGGACCACTGCATTGAGATTTTAAGCCATGACAAAGATTACAACAAGGTTAA
                     GCTGTATGAGCATGCTGTTGCTCATTCTGGATTGCCTGACAATGCCAGACGA"
     primer_bind     320..339
                     /standard_name="1,307 F"
     misc_feature    complement(587..600)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="600 R"
     misc_feature    complement(587..>600)
                     /standard_name="puroR reverse"
     misc_feature    <601..>609
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pSLQ1501_Actual_ (modified) -
                     Fragment 3 (concatenated sequence 1)"
     misc_feature    601..>609
                     /standard_name="P2A(1)"
     misc_feature    601..609
                     /standard_name="GSG linker"
     misc_feature    601..609
                     /standard_name="GSG linker(1)"
     misc_feature    <610..>666
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pSLQ1501_Actual_ (modified) -
                     Fragment 3 (concatenated sequence 1)"
     misc_feature    <610..666
                     /standard_name="P2A(1)"
     misc_feature    610..666
                     /standard_name="P2A"
     misc_feature    complement(610..648)
                     /standard_name="p2a r hom arm"
     primer_bind     610..628
                     /standard_name="751 F"
     misc_feature    628..666
                     /standard_name="p2a f hom arm"
     misc_feature    complement(648..666)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="744 R"
     source          <667..>672
     misc_feature    673..699
                     /standard_name="HA"
     primer_bind     673..693
                     /standard_name="865 F"
     primer_bind     673..691
                     /standard_name="865 F"
     misc_feature    complement(679..699)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="699 R"
     misc_feature    complement(680..699)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="699 R"
     misc_feature    complement(681..699)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="699 R"
     misc_feature    complement(682..699)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="699 R"
     primer_bind     700..720
                     /standard_name="1 F"
     primer_bind     700..719
                     /standard_name="1 F"
     primer_bind     700..718
                     /standard_name="1 F"
     misc_feature    complement(1561..1581)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="882 R"
     misc_feature    complement(1562..1581)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="882 R"
     misc_feature    1582..1611
                     /standard_name="GS linker"
     misc_feature    complement(1592..1611)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="1,794 R"
ORIGIN      
        1 atggccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
       61 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
      121 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
      181 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
      241 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
      301 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
      361 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
      421 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
      481 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
      541 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
      601 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
      661 ggacctaagc tttacccata tgacgtgcca gactacgccg aaatcggtac tggctttcca
      721 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
      781 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
      841 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg
      901 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
      961 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
     1021 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
     1081 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
     1141 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
     1201 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
     1261 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
     1321 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
     1381 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
     1441 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg
     1501 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
     1561 ctgtctactc tggagatttc cggaggaggt gggtctggag gtggaggatc t
//
