LOCUS       c747pCS2+_PLA2-mk2      7047 bp ds-DNA     circular     17-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1893..1910
                     /label="Sp6_primer"
     misc_feature    complement(5236..5254)
                     /label="M13_reverse_primer"
     primer_bind     complement(2327..2348)
                     /label="EB_pr131R"
     misc_feature    1956..4178
                     /label="cPLA2"
     promoter        complement(256..284)
                     /label="AmpR_promoter"
     primer_bind     1956..1980
                     /label="EB_pr131F"
     misc_feature    2156..2156
                     /label="PCR\mut\A-->G"
     misc_feature    complement(5253..5275)
                     /label="M13_pUC_rev_primer"
     rep_origin      complement(477..783)
                     /label="f1_origin"
     promoter        complement(5200..5219)
                     /label="T3_promoter"
     misc_feature    1776..1817
                     /label="CMV_fwd_primer"
     rep_origin      complement(5627..6246)
                     /label="pBR322_origin"
     misc_feature    1956..4916
                     /label="cPLA2-mks"
     misc_feature    complement(4978..4997)
                     /label="EBV_rev_primer"
     gene            complement(join(6401..7047,1..214))
                     /label="Ampicillin"
     promoter        complement(5289..5318)
                     /label="lac_promoter"
     misc_feature    1956..2348
                     /label="C2 domain"
     CDS             complement(join(6401..7047,1..214))
                     /label="Ampicillin"
     misc_feature    4206..4913
                     /label="mK2"
     terminator      complement(4964..5083)
                     /label="SV40_PA_terminator"
ORIGIN
        1 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
       61 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
      121 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
      181 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
      241 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
      301 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA
      361 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC
      421 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT
      481 TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA
      541 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG
      601 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG
      661 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC
      721 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA
      781 TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
      841 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GTCGACCATA GCCAATTCAA TATGGCGTAT
      901 ATGGACTCAT GCCAATTCAA TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT
      961 ATGGACTCGT GCCAATTCAA TATGGTGGAT CTGGACCCCA GCCAATTCAA TATGGCGGAC
     1021 TTGGCACCAT GCCAATTCAA TATGGCGGAC TTGGCACTGT GCCAACTGGG GAGGGGTCTA
     1081 CTTGGCACGG TGCCAAGTTT GAGGAGGGGT CTTGGCCCTG TGCCAAGTCC GCCATATTGA
     1141 ATTGGCATGG TGCCAATAAT GGCGGCCATA TTGGCTATAT GCCAGGATCA ATATATAGGC
     1201 AATATCCAAT ATGGCCCTAT GCCAATATGG CTATTGGCCA GGTTCAATAC TATGTATTGG
     1261 CCCTATGCCA TATAGTATTC CATATATGGG TTTTCCTATT GACGTAGATA GCCCCTCCCA
     1321 ATGGGCGGTC CCATATACCA TATATGGGGC TTCCTAATAC CGCCCATAGC CACTCCCCCA
     1381 TTGACGTCAA TGGTCTCTAT ATATGGTCTT TCCTATTGAC GTCATATGGG CGGTCCTATT
     1441 GACGTATATG GCGCCTCCCC CATTGACGTC AATTACGGTA AATGGCCCGC CTGGCTCAAT
     1501 GCCCATTGAC GTCAATAGGA CCACCCACCA TTGACGTCAA TGGGATGGCT CATTGCCCAT
     1561 TCATATCCGT TCTCACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC ACTTGGCAGT
     1621 ACATCAATAT CTATTAATAG TAACTTGGCA AGTACATTAC TATTGGAAGG ACGCCAGGGT
     1681 ACATTGGCAG TACTCCCATT GACGTCAATG GCGGTAAATG GCCCGCGATG GCTGCCAAGT
     1741 ACATCCCCAT TGACGTCAAT GGGGAGGGGC AATGACGCAA ATGGGCGTTC CATTGACGTA
     1801 AATGGGCGGT AGGCGTGCCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAACCG
     1861 CCATTCTGCC TGGGGACGTC GGAGCAAGCT TGATTTAGGT GACACTATAG AATACAAGCT
     1921 ACTTGTTCTT TTTGCAGGAT CCCATCGATG CCACAATGTC CAACATTATA GTTGAGCATC
     1981 AGTATTCTCA TAGACTGAAA TTGACTGTGG TGAGAGCTGA AAATGTAACT AAAGGAGCAT
     2041 TTGGGGATTT ATTGGACACC CCGGACCCTT ATGTTGAGCT GTCAGTGCCA ACCACACCAG
     2101 AGTCAAGAAA ACGAACCCGC CACATTAACA ATGACATTAA CCCCAAGTGG AACGAGACCT
     2161 TTGAGTTCAT ATTGGATCCC AATCAATCCA ATGTTTTGGA GGTGACACTG ATGGATGCCA
     2221 ATTATGTCAT GGATGAAACT CTTGGCACAG CAAAATATTC CCTCTCAAAA CTCAAAGTGG
     2281 CACAAATGGA GCATGTGACC CTCTCTATCG GCAAGACAAC CAAAGTGTTC CTCGACTTGC
     2341 TCTTGGAAGT TTGCGCATCC ACAGACCTGC GCTTCAGCAT GACACTGTGT GATCAAGAGA
     2401 AGCTGTTCAT GCAGACACGC AGAGACAGGG TCATGCTCAG CATAAAGAAG CTTCTTAAAA
     2461 TGGAAAACCC TCGTTTCCTT CCATCCTCAC CAAGAGAGGT TCCCACCATC GCTATTCTGG
     2521 GCTCTGGAGG AGGTTTTCGT GCAATGGTGG GCTTCTCTGG TGTGATGAAG GCTTTGTATG
     2581 AATCTGGCGT GTTTGACTGT GCAACATATG TGGCAGGACT TTCAGGATCA ACATGGTATA
     2641 TGTCTATGCT GTACTCTCAT CCAGAGTTTC CTGCTAAAGG CCCAGGCGAC ATTAATAAAG
     2701 AGCTCATGAA CAGAGTCAGT AATAACCCAC TCAAACTGCT CCTGCCCCAA AACATCAACC
     2761 GCTACGTCAA AGCACTGTGG AAGAAGAAAT CTGCTGGACA GCCGGTCACC TTCACTGACA
     2821 TCTTCGGGAT GTTGATAGGA GAGACGCTTA TTCCAGGGAG AATGAACATT AAGCTGAGCT
     2881 CATTGAAAGG GAAAATTAAT GAAGGTCAGA GCCCTCTTCC TCTCTTCACC TGTCTGCACG
     2941 TCAAACCTGA CGTCTCTGAG CTCATGTTTG CAGATTGGGT GGAGTTCAGC CCTTATGAAA
     3001 TTGGCATGGC CAAATATGGC ACCTTCATGT CTCCTGGGCT GTTTGGCAGC AAATTCTTCA
     3061 TGGGAAGTGT TGTGAAACAA TATGAGGAAA ACCCCTTACA TTTCCTGATG GGTGTGTGGG
     3121 GAAGTGCTTT TTCCATCCTC TTTAACCGGG TTTTGGGTGT GAAGGAGACG ACCAGCAGTA
     3181 GCACTATGGA GGAGGAGCTG GAGCAAATTA AACCAGAGCA CATTGTGGGT GACGATTCTG
     3241 CTGATAACGA GGAGGAAACT CAAAGAGGAG GCACAGAGTC TGCAGATGCA GAGGACGAGC
     3301 GCCAGCGTCA TGCCCAGGCC AGCTGGGTAC AGCGCATGTT GACCTCCATC ATGGGCGACA
     3361 CCACTCTGTT CACCACTCGT GAGGGCCGCG CAGGCAAAGT GCACAACTTC ATGCTGGGAC
     3421 TGAACCTGAA CTCAACCCTG CCCTTCTCTC CCTTCAGCGG CATCACACAC CAGACCTCGC
     3481 TGGAGGAGGA AGTGGACGCT GTTACTGATC CGGATGAGTT TGAGCGAATC TATGAGCCTC
     3541 TGGATGTGAA GAGCAAGAAA ATCCATGTGG TGGACAGTGG CTTGACCTTT AACCTGCCCT
     3601 ACCCTCTCAT TCTGCGGTGT CAGAGAGGAG TCGACCTGAT TATTTCATTC GACTTCTCAG
     3661 CCCGACCCAG TGACTCCAGC CCACCGTTCA AAGAGTTACT GCTGGCTGAA AAATGGGCCC
     3721 GTATGAATAA GCTGCCCTTC CCGAAGATTG ATTCGAAAGT ATTCGACAGA GAGGGTCTAA
     3781 AAGAGTGCTA TGTGTTCAAG CCTGCTAAAG GAGACAAGAA CTGCCCCACC ATCATTCATT
     3841 TTGTCTTGGC CAACATCAAC TTCCGGAATT TTAAAGCCCC TGGAGTTCCT CGAGATTCAG
     3901 ACAAGGACAT AGAGTTTGGT GACTTTGACA TATTTGACGA GCCAGCCTCC CCTTACTCCA
     3961 CTTTCAACTT CAAATACAAC AACCAGGCCT TCAAGAGACT TCATGACCTC ATGGAGTTCA
     4021 ATACGCTTAA CAACATCGAG GTTATCAAAG AAGCCATTAA AGACAGCATT CTGCTGAGAC
     4081 GAGAGAACCC AGCGCGATGT TCGGTTTCCC TTTCGCTCAG TGAAATCGAA AACAAGAAGT
     4141 TCCTGAAACG AGACAACAGC ATTGCAAAAC GACCCACCGG AGCTGGAGGA ATTCGTCCTG
     4201 GATCTATGGT GTCTAAGGGC GAAGAGCTGA TTAAGGAGAA CATGCACATG AAGCTGTACA
     4261 TGGAGGGCAC CGTGAACAAC CACCACTTCA AGTGCACATC CGAGGGCGAA GGCAAGCCCT
     4321 ACGAGGGCAC CCAGACCATG AGAATCAAGG CCGTCGAGGG CGGCCCTCTC CCCTTCGCCT
     4381 TCGACATCCT GGCTACCAGC TTCATGTACG GCAGCAAAAC CTTCATCAAC CACACCCAGG
     4441 GCATCCCCGA CTTCTTTAAG CAGTCCTTCC CTGAGGGCTT CACATGGGAG AGAGTCACCA
     4501 CATACGAAGA CGGGGGCGTG CTGACCGCTA CCCAGGACAC CAGCCTCCAG GACGGCTGCC
     4561 TCATCTACAA CGTCAAGATC AGAGGGGTGA ACTTCCCATC CAACGGCCCT GTGATGCAGA
     4621 AGAAAACACT CGGCTGGGAG GCCTCCACCG AGACCCTGTA CCCCGCTGAC GGCGGCCTGG
     4681 AAGGCAGAGC CGACATGGCC CTGAAGCTCG TGGGCGGGGG CCACCTGATC TGCAACTTGA
     4741 AGACCACATA CAGATCCAAG AAACCCGCTA AGAACCTCAA GATGCCCGGC GTCTACTATG
     4801 TGGACAGAAG ACTGGAAAGA ATCAAGGAGG CCGACAAAGA GACCTACGTC GAGCAGCACG
     4861 AGGTGGCTGT GGCCAGATAC TGCGACCTCC CTAGCAAACT GGGGCACAAA CTGTAACTCG
     4921 AGCCTCTAGA ACTATAGTGA GTCGTATTAC GTAGATCCAG ACATGATAAG ATACATTGAT
     4981 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     5041 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     5101 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC
     5161 GCGGCGCCAA TGCATTGGGC CCGGTACCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG
     5221 CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
     5281 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
     5341 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
     5401 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
     5461 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
     5521 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
     5581 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
     5641 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
     5701 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
     5761 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
     5821 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
     5881 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
     5941 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
     6001 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
     6061 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
     6121 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
     6181 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
     6241 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
     6301 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
     6361 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
     6421 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
     6481 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
     6541 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
     6601 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
     6661 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
     6721 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
     6781 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
     6841 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
     6901 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
     6961 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
     7021 GGGATAATAC CGCGCCACAT AGCAGAA
//