LOCUS       pXPR-062_hygro-2a-tagbf 9609 bp ds-DNA     circular     28-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             7888..8123
                     /label="3'\LTR\SIN"
     misc_feature    6481..7179
                     /label="tagBFP"
     promoter        3483..3723
                     /label="U6 promoter"
     misc_feature    complement(3919..3943)
                     /label="Kermit"
     rep_origin      8703..9158
                     /label="F1 ori"
     misc_feature    3712..3732
                     /label="Argon"
     misc_feature    4100..5358
                     /label="EF1a"
     misc_RNA        3753..3828
                     /label="gRNA scaffold"
     rep_origin      8677..9107
                     /label="F1\ORI"
     misc_feature    3709..3725
                     /label="Neon"
     misc_feature    2893..2913
                     /label="RRE_for primer"
     misc_feature    2841..3082
                     /label="RRE"
     misc_feature    complement(7842..7866)
                     /label="afterWPRE_rev primer"
     rep_origin      8410..8485
                     /label="SV40 origin of replication"
     misc_feature    3890..4007
                     /label="cPPT/CTS"
     misc_feature    3732..3752
                     /label="BsmBIx2-6T"
     misc_binding    7229..7817
                     /label="WPRE"
     misc_feature    4066..4099
                     /label="lox2272"
     CDS             join(9289..9609,1..540)
                     /label="AmpR"
     misc_feature    2227..2364
                     /label="psi"
     rep_origin      688..1355
                     /label="pUC ori"
     misc_feature    2331..2695
                     /label="gag"
     misc_feature    6409..6474
                     /label="P2A"
     misc_feature    7180..7213
                     /label="lox2272"
     LTR             1764..2176
                     /label="5'LTR"
     polyA_signal    8200..8330
                     /label="SV40 polyadenylation signal"
     misc_feature    5374..6402
                     /label="Hygro"
ORIGIN
        1 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
       61 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
      301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
      361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
      421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
      481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
      541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1501 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1561 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1621 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1681 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     1741 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     1801 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     1861 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     1921 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     1981 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2041 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2101 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2161 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     2221 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     2281 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     2341 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     2401 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     2461 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     2521 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     2581 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     2641 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     2701 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     2761 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     2821 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     2881 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     2941 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     3001 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     3061 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     3121 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     3181 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     3241 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     3301 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     3361 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     3421 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccggta
     3481 ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     3541 agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     3601 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     3661 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     3721 gacgaaacac cggagacgct tttttcgtct cagtttgaga gctagaaata gcaagttcaa
     3781 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc
     3841 aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     3901 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3961 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4021 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggataac ttcgtatagg
     4081 atactttata cgaagttatT GCAAAGATGG ATAAAGTTTT AAACAGAGAG GAATCTTTGC
     4141 AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC CCGTCAGTGG
     4201 GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGATCC
     4261 GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC
     4321 CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT
     4381 TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT
     4441 GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACCTG GCTGCAGTAC
     4501 GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT
     4561 TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC
     4621 GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT TCGATAAGTC TCTAGCCATT
     4681 TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC AAGATAGTCT TGTAAATGCG
     4741 GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT
     4801 GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG CGCGGCCACC GAGAATCGGA
     4861 CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG GCCTCGCGCC GCCGTGTATC
     4921 GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG TTGCGTGAGC GGAAAGATGG
     4981 CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA CGCGGCGCTC GGGAGAGCGG
     5041 GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT CCTCAGCCGT CGCTTCATGT
     5101 GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT AGTTCTCGAG CTTTTGGAGT
     5161 ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG
     5221 GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT
     5281 TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT
     5341 CCATTTCAGG TGTCGTGACG TACGGCCACC ACCATGAAGA AGCCTGAGCT GACCGCCACA
     5401 TCCGTGGAGA AGTTCCTGAT CGAGAAGTTT GACTCTGTGA GCGATCTGAT GCAGCTGTCT
     5461 GAGGGCGAGG AGAGCAGGGC CTTCAGCTTC GACGTGGGCG GAAGGGGCTA CGTGCTGAGA
     5521 GTGAACAGCT GCGCCGACGG ATTCTACAAG GATAGATACG TGTACCGCCA CTTTGCTTCC
     5581 GCCGCTCTGC CAATCCCTGA GGTGCTGGAC ATCGGCGAGT TCTCCGAGTC TCTGACCTAC
     5641 TGTATCAGCA GGAGAGCCCA GGGAGTGACC CTGCAGGATC TGCCTGAGAC AGAGCTGCCA
     5701 GCCGTGCTGC AGCCAGTGGC TGAGGCTATG GACGCTATCG CCGCTGCCGA TCTGTCTCAG
     5761 ACAAGCGGCT TCGGACCCTT TGGCCCTCAG GGCATCGGAC AGTACACCAC ATGGCGCGAC
     5821 TTCATCTGCG CTATCGCCGA TCCCCACGTG TACCACTGGC AGACCGTGAT GGACGATACA
     5881 GTGAGCGCCT CCGTGGCTCA GGCCCTGGAC GAGCTGATGC TGTGGGCCGA GGATTGTCCT
     5941 GAGGTGCGGC ACCTGGTGCA CGCTGACTTC GGCTCCAACA ACGTGCTGAC CGATAACGGA
     6001 CGCATCACAG CCGTGATCGA CTGGAGCGAG GCTATGTTTG GCGATTCCCA GTACGAGGTG
     6061 GCCAACATCT TCTTTTGGAG GCCATGGCTG GCTTGCATGG AGCAGCAGAC CAGATACTTC
     6121 GAGCGGCGCC ACCCTGAGCT GGCTGGATCT CCAAGGCTGA GAGCTTACAT GCTGAGGATC
     6181 GGACTGGACC AGCTGTACCA GAGCCTGGTG GATGGCAACT TTGACGATGC TGCTTGGGCT
     6241 CAGGGACGGT GCGACGCTAT CGTGCGCTCC GGAGCTGGAA CCGTGGGAAG AACACAGATC
     6301 GCCAGGAGAT CTGCTGCCGT GTGGACAGAC GGATGCGTGG AGGTGCTGGC TGATTCTGGA
     6361 AACCGGCGCC CAAGCACCCG GCCCCGCGCT AAGGACCTGC AGCTGCAGGG ATCCGGAGCT
     6421 ACAAACTTCT CTCTGCTGAA GCAGGCTGGC GATGTGGAGG AGAACCCAGG ACCCGCCAGC
     6481 TCCGAGCTGA TCAAGGAGAA CATGCACATG AAGCTGTACA TGGAGGGCAC CGTGGACAAC
     6541 CACCACTTTA AGTGCACATC TGAGGGCGAG GGAAAGCCCT ACGAGGGAAC CCAGACAATG
     6601 CGGATCAAGG TGGTGGAGGG CGGACCTCTG CCATTCGCTT TTGACATCCT GGCCACCAGC
     6661 TTCCTGTACG GCTCCAAGAC CTTTATCAAC CACACACAGG GAATCCCAGA CTTCTTTAAG
     6721 CAGTCCTTCC CCGAGGGCTT TACCTGGGAG CGGGTGACCA CATACGAGGA TGGCGGAGTG
     6781 CTGACCGCCA CACAGGACAC AAGCCTGCAG GATGGATGTC TGATCTACAA CGTGAAGATC
     6841 CGCGGCGTGA ACTTCACCTC CAACGGACCT GTGATGCAGA AGAAGACACT GGGCTGGGAG
     6901 GCCTTTACCG AGACACTGTA CCCAGCTGAC GGCGGACTGG AGGGAAGGAA CGATATGGCC
     6961 CTGAAGCTGG TGGGCGGATC TCACCTGATC GCTAACATCA AGACCACATA CAGAAGCAAG
     7021 AAGCCCGCCA AGAACCTGAA GATGCCTGGC GTGTACTACG TGGACTACAG GCTGGAGAGA
     7081 ATCAAGGAGG CTAACAACGA GACCTACGTG GAGCAGCACG AGGTGGCTGT GGCCAGATAC
     7141 TGTGATCTGC CAAGCAAGCT GGGCCACAAG CTGAATTAGa taacttcgta taggatactt
     7201 tatacgaagt tatacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7261 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7321 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7381 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7441 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7501 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7561 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7621 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7681 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7741 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7801 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     7861 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7921 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7981 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8041 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8101 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     8161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8341 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8401 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8461 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8521 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8581 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8641 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8701 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8761 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8821 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8881 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8941 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9001 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9061 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9121 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     9181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9601 ccagtcaca
//