LOCUS       Exported                8868 bp ds-DNA     circular SYN 01-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   VB200609-1075zxu
VERSION     .
KEYWORDS    VB200609-1075zxu.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 13, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/description=['Rous sarcoma virus 
                     enhancer/promoter']"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
                     /note="/vntifkey=21"
     LTR             230..410
                     /label=5' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/description=[Truncated HIV-1 5' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-DeltaU3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
                     /note="/vntifkey=19"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/description=['HIV-1 packaging signal']"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
                     /note="/vntifkey=21"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=['HIV-1 Rev response element']"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="/vntifkey=21"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=['Central polypurine tract']"
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
                     /note="/vntifkey=21"
     promoter        1959..3137
                     /label=EF1A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter."
                     /note="/description=['Human eukaryotic translation 
                     elongationfactor 1 alpha1 promoter']"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="/vntifkey=21"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=['Kozak translation initiation 
                     sequence']"
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
     misc_feature    3168..3764
                     /label=Puro(ns)
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=['Puromycin resistance gene']"
                     /note="/full_name=Puromycin resistance gene(ns)"
                     /note="/name=Puro(ns)"
                     /note="/official_designation=Puro(ns)"
                     /note="/uuid=af7065d688f147098574d240fe54448c"
                     /note="/vntifkey=21"
     misc_feature    3765..3827
                     /label=T2A
                     /label=nonstandard type:  Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=['Self-cleaving 2A peptide from Thosea 
                     asignavirus']"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/name=T2A"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
                     /note="/vntifkey=21"
     misc_feature    3828..4544
                     /label=EGFP
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used green 
                     fluorescentprotein; ranked high in brightness, 
                     photostability and pH stability among all fluorescent 
                     proteins."
                     /note="/description=['Enhanced green fluorescent protein; 
                     codonoptimized based on a variant of wild type GFP from the
                     jellyfish Aequorea victoria']"
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/name=EGFP"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
                     /note="/vntifkey=21"
     misc_feature    4545..4574
                     /label=Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Linker"
                     /note="/official_designation=Linker"
                     /note="/uuid=85f26adf9fd241219e0328d21bb04a62"
                     /note="/vntifkey=21"
     misc_feature    4575..4607
                     /label=VSV-G tag
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=VSV-G tag"
                     /note="/official_designation=VSV-G tag"
                     /note="/uuid=15321171b131444da46d16236b584d8d"
                     /note="/vntifkey=21"
     misc_feature    4608..4643
                     /label=ProtC
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=ProtC"
                     /note="/official_designation=ProtC"
                     /note="/uuid=fe4521aafb1e4da0a9121c4af2e0c420"
                     /note="/vntifkey=21"
     misc_feature    4644..4667
                     /label=FLAG
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates antibody binding of 
                     taggedprotein."
                     /note="/name=FLAG"
                     /note="/official_designation=FLAG"
                     /note="/uuid=474fcfa7319544cf883e53bf1ac6dde0"
                     /note="/vntifkey=21"
     misc_feature    4709..5306
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=['Woodchuck hepatitis 
                     virusposttranscriptional regulatory element']"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="/vntifkey=21"
     misc_feature    5183^5184
                     /label=No marker
                     /label=nonstandard type:  Marker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/application_notes="
                     /note="/name=No marker"
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
                     /note="/vntifkey=21"
     LTR             5388..5622
                     /label=3' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=[Truncated HIV-1 3' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-DeltaU3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
                     /note="/vntifkey=19"
     polyA_signal    5695..5829
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=['Simian virus 40 early 
                     polyadenylationsignal']"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
                     /note="/vntifkey=21"
     misc_feature    6783..7643
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=['AmpiciIIin resistance gene']"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpiciIIin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
     rep_origin      7814..8402
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=['pUC origin of replication']"
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
                     /note="/vntifkey=21"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg accgagtaca
     3181 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3241 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     3301 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3361 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     3421 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3481 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3541 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3601 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3661 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3721 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggaagc ggagagggca
     3781 ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc cggccccatg gtgagcaagg
     3841 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3901 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3961 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4021 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4081 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4141 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4201 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4261 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4321 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4381 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4441 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4501 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggc agcggcggcg
     4561 gcggctctgg cggctacacc gacatcgaga tgaatcgtct gggcaaggaa gatcaagttg
     4621 atcctcgtct gatcgacggc aaggactaca aagacgatga cgacaagtaa acccagcttt
     4681 cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac aaaatttgtg
     4741 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4801 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4861 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4921 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4981 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5041 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5101 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5161 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5221 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5281 ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgctttaaga ccaatgactt
     5341 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5401 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5461 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5521 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5581 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5641 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5821 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5941 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6001 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6061 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6181 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6241 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6301 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6361 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6421 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6481 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6541 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6601 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6661 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6721 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6781 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6841 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6901 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6961 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7021 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7081 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7141 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7201 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7261 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7321 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7381 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7441 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7501 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7561 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7621 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7681 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7741 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7921 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8221 cgcttcccga agagagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8521 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8581 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8701 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8761 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8821 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
