LOCUS       Exported                8853 bp ds-DNA     circular SYN 01-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   VB200609-1071egs
VERSION     .
KEYWORDS    VB200609-1071egs.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 13, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/description=['Rous sarcoma virus 
                     enhancer/promoter']"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
                     /note="/vntifkey=21"
     LTR             230..410
                     /label=5' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/description=[Truncated HIV-1 5' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-DeltaU3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
                     /note="/vntifkey=19"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/description=['HIV-1 packaging signal']"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
                     /note="/vntifkey=21"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=['HIV-1 Rev response element']"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="/vntifkey=21"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=['Central polypurine tract']"
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
                     /note="/vntifkey=21"
     promoter        1959..3137
                     /label=EF1A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter."
                     /note="/description=['Human eukaryotic translation 
                     elongationfactor 1 alpha1 promoter']"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="/vntifkey=21"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=['Kozak translation initiation 
                     sequence']"
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
     misc_feature    3168..3764
                     /label=Puro(ns)
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=['Puromycin resistance gene']"
                     /note="/full_name=Puromycin resistance gene(ns)"
                     /note="/name=Puro(ns)"
                     /note="/official_designation=Puro(ns)"
                     /note="/uuid=af7065d688f147098574d240fe54448c"
                     /note="/vntifkey=21"
     misc_feature    3765..3827
                     /label=T2A
                     /label=nonstandard type:  Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=['Self-cleaving 2A peptide from Thosea 
                     asignavirus']"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/name=T2A"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
                     /note="/vntifkey=21"
     misc_feature    3828..4544
                     /label=EGFP
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used green 
                     fluorescentprotein; ranked high in brightness, 
                     photostability and pH stability among all fluorescent 
                     proteins."
                     /note="/description=['Enhanced green fluorescent protein; 
                     codonoptimized based on a variant of wild type GFP from the
                     jellyfish Aequorea victoria']"
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/name=EGFP"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
                     /note="/vntifkey=21"
     misc_feature    4545..4574
                     /label=Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Linker"
                     /note="/official_designation=Linker"
                     /note="/uuid=85f26adf9fd241219e0328d21bb04a62"
                     /note="/vntifkey=21"
     misc_feature    4575..4607
                     /label=VSV-G tag
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=VSV-G tag"
                     /note="/official_designation=VSV-G tag"
                     /note="/uuid=15321171b131444da46d16236b584d8d"
                     /note="/vntifkey=21"
     misc_feature    4608..4634
                     /label=HA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=HA"
                     /note="/official_designation=HA"
                     /note="/uuid=e81c4508bc964b63a8baef6969045003"
                     /note="/vntifkey=21"
     misc_feature    4635..4652
                     /label=AU1
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=AU1"
                     /note="/official_designation=AU1"
                     /note="/uuid=d60e2f0b640841df9b7158581c44340c"
                     /note="/vntifkey=21"
     misc_feature    4694..5291
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=['Woodchuck hepatitis 
                     virusposttranscriptional regulatory element']"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="/vntifkey=21"
     misc_feature    5183^5184
                     /label=No marker
                     /label=nonstandard type:  Marker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/application_notes="
                     /note="/name=No marker"
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
                     /note="/vntifkey=21"
     LTR             5373..5607
                     /label=3' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=[Truncated HIV-1 3' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-DeltaU3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
                     /note="/vntifkey=19"
     polyA_signal    5680..5814
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=['Simian virus 40 early 
                     polyadenylationsignal']"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
                     /note="/vntifkey=21"
     misc_feature    6768..7628
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=['AmpiciIIin resistance gene']"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpiciIIin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
     rep_origin      7799..8387
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=['pUC origin of replication']"
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
                     /note="/vntifkey=21"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg accgagtaca
     3181 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3241 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     3301 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3361 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     3421 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3481 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3541 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3601 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3661 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3721 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggaagc ggagagggca
     3781 ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc cggccccatg gtgagcaagg
     3841 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3901 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3961 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4021 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4081 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4141 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4201 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4261 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4321 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4381 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4441 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4501 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggc agcggcggcg
     4561 gcggctctgg cggctacacc gacatcgaga tgaatcgtct gggcaagtac ccctacgacg
     4621 tgcccgacta cgccgacacc tacagataca tctaaaccca gctttcttgt acaaagtggt
     4681 gataatcgaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4741 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4801 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4861 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4921 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4981 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5041 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5101 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5161 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5221 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5281 ccccgcatcg ggaattcccg cggttcgctt taagaccaat gacttacaag gcagctgtag
     5341 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5401 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5461 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5521 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5581 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5641 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5701 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5761 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5941 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6001 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6061 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6181 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6241 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6361 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6541 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6601 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6841 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6961 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7021 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7081 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7141 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7201 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7261 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7321 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7381 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7441 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7501 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7561 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7621 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7921 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga
     8221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8461 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8521 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8581 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8641 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8701 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8761 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8821 actaaaggga acaaaagctg gagctgcaag ctt
//
