LOCUS       Exported                8844 bp ds-DNA     circular SYN 05-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   VB200609-1101ped
VERSION     .
KEYWORDS    VB200609-1101ped.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 13, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter; drives 
                     transcriptionof viral RNA in packaging cells."
                     /note="/description=['Rous sarcoma virus 
                     enhancer/promoter']"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/name=RSV  promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
                     /note="/vntifkey=21"
     LTR             230..410
                     /label=5' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription of viral RNA
                     andits packaging into virus."
                     /note="/description=[Truncated HIV-1 5' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/name=5' LTR-DeltaU3"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
                     /note="/vntifkey=19"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus."
                     /note="/description=['HIV-1 packaging signal']"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/name=Psi"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
                     /note="/vntifkey=21"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Rev protein binding site that 
                     allowsRev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=['HIV-1 Rev response element']"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/name=RRE"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
                     /note="/vntifkey=21"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1cDNA through a central DNA flap."
                     /note="/description=['Central polypurine tract']"
                     /note="/full_name=Central polypurine tract"
                     /note="/name=cPPT"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
                     /note="/vntifkey=21"
     promoter        1959..3137
                     /label=EF1A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter."
                     /note="/description=['Human eukaryotic translation 
                     elongationfactor 1 alpha1 promoter']"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="/vntifkey=21"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=['Kozak translation initiation 
                     sequence']"
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
     misc_feature    3168..3764
                     /label=Puro(ns)
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     topuromycin."
                     /note="/description=['Puromycin resistance gene']"
                     /note="/full_name=Puromycin resistance gene(ns)"
                     /note="/name=Puro(ns)"
                     /note="/official_designation=Puro(ns)"
                     /note="/uuid=af7065d688f147098574d240fe54448c"
                     /note="/vntifkey=21"
     misc_feature    3765..3827
                     /label=T2A
                     /label=nonstandard type:  Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=['Self-cleaving 2A peptide from Thosea 
                     asignavirus']"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/name=T2A"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
                     /note="/vntifkey=21"
     misc_feature    3828..4535
                     /label=mCherry
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used red fluorescent 
                     protein;fast maturation compared to its predecessor, 
                     mRFP1."
                     /note="/full_name=mCherry(ns)"
                     /note="/name=mCherry"
                     /note="/official_designation=mCherry(ns)"
                     /note="/uuid=fa8215a40d51475bac87b439f15e8a4d"
                     /note="/vntifkey=21"
     misc_feature    4536..4565
                     /label=Linker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Linker"
                     /note="/official_designation=Linker"
                     /note="/uuid=85f26adf9fd241219e0328d21bb04a62"
                     /note="/vntifkey=21"
     misc_feature    4566..4601
                     /label=ProtC
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=ProtC"
                     /note="/official_designation=ProtC"
                     /note="/uuid=fe4521aafb1e4da0a9121c4af2e0c420"
                     /note="/vntifkey=21"
     misc_feature    4602..4625
                     /label=FLAG
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates antibody binding of 
                     taggedprotein."
                     /note="/name=FLAG"
                     /note="/official_designation=FLAG"
                     /note="/uuid=474fcfa7319544cf883e53bf1ac6dde0"
                     /note="/vntifkey=21"
     misc_feature    4626..4643
                     /label=AU1
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=AU1"
                     /note="/official_designation=AU1"
                     /note="/uuid=d60e2f0b640841df9b7158581c44340c"
                     /note="/vntifkey=21"
     misc_feature    4685..5282
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=['Woodchuck hepatitis 
                     virusposttranscriptional regulatory element']"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="/vntifkey=21"
     misc_feature    5183^5184
                     /label=No marker
                     /label=nonstandard type:  Marker
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/application_notes="
                     /note="/name=No marker"
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
                     /note="/vntifkey=21"
     LTR             5364..5598
                     /label=3' LTR-DeltaU3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows packaging of viral RNA 
                     intovirus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=[Truncated HIV-1 3' long terminal 
                     repeat]"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/name=3' LTR-DeltaU3"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
                     /note="/vntifkey=19"
     polyA_signal    5671..5805
                     /label=SV40 early pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=['Simian virus 40 early 
                     polyadenylationsignal']"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/name=SV40 early pA"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
                     /note="/vntifkey=21"
     misc_feature    6759..7619
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/description=['AmpiciIIin resistance gene']"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpiciIIin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
     rep_origin      7790..8378
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=['pUC origin of replication']"
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
                     /note="/vntifkey=21"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg accgagtaca
     3181 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3241 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     3301 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3361 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     3421 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3481 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3541 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3601 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3661 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3721 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggaagc ggagagggca
     3781 ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc cggccccatg gtgagcaagg
     3841 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3901 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3961 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4021 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4081 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4141 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4201 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4261 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4321 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4381 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4441 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4501 gccactccac cggcggcatg gacgagctgt acaagggtgg cagcggcggc ggcggctctg
     4561 gcggcgaaga tcaagttgat cctcgtctga tcgacggcaa ggactacaaa gacgatgacg
     4621 acaaggacac ctacagatac atctaaaccc agctttcttg tacaaagtgg tgataatcga
     4681 attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4741 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4801 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4861 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4921 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4981 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5041 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5101 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5161 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5221 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5281 gggaattccc gcggttcgct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5401 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5461 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5521 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5581 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5641 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5701 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5761 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5821 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5881 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5941 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6001 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6061 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6121 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6361 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6481 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6601 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6661 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6721 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6781 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6841 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6901 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6961 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7021 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7081 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7141 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7201 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7261 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7321 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7381 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7441 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7501 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7561 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7621 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7681 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7921 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg
     8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8581 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8641 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8701 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8761 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8821 aacaaaagct ggagctgcaa gctt
//
