LOCUS       Exported               11509 bp ds-DNA     circular SYN 10-MAY-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    550..729
                     /label=cPPT
                     /note="gene=""cPPT"" - vntifkey=""34"" - label=""cPPT"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     550..729"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     609..630
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="vntifkey=""27"" - label=""cPPT_5'seq"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     705..728"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     738..772
                     /label=EcoEF1a_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattccgtgaggctccggtgcccg"
     promoter        754..1937
                     /label=EF-1alpha
                     /note="vntifkey=""29"" - label=EF-1alpha - 
                     ApEinfo_fwdcolor=#ff8040 - ApEinfo_revcolor=green - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 234..1417"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     1321..1340
                     /label=QB4469
                     /note="color: black; sequence: GCCAAGATCTGCACACTGGT"
     primer_bind     1444..1462
                     /label=QB1511
                     /note="color: black; sequence: GGTAGTCTCAAGCTGGCCG"
     primer_bind     1885..1905
                     /label=EF1a forward primer binding (QB0019)
                     /note="label=EF1a forward primer binding (QB0019) - 
                     ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=cyan - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1365..1385"
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(1923..1950)
                     /label=EcoEF1a_Rv
                     /note="color: black; sequence: 
                     tgacgcgtgagaattctcacgacacctgaaa"
     primer_bind     complement(1928..1950)
                     /label=pHR_EcoRIhomo_EF1a_rev
                     /note="color: black; sequence: 
                     TTGacgcgtgagaattCGCTAGCctcacgacacc"
     primer_bind     1939..1965
                     /label=sGFPFc_Fw
                     /note="color: black; sequence: aattctcacgcggccGCCACCATGGGC"
     primer_bind     1939..1965
                     /label=solGFP_Fw
                     /note="color: black; sequence: aattctcacgcgGCCGCCACCATGGGC"
     misc_feature    1951..1959
                     /label=KOZAK
                     /note="color: #a6acb3"
     misc_feature    1960..2010
                     /label=signal sequence (Gaussia Luciferase)
                     /note="color: #000000"
     CDS             2011..2724
                     /codon_start=1
                     /note="color: #000000"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    2011..2724
                     /label=EGFP
                     /note="color: #00ff00"
     primer_bind     2011..2038
                     /label=19GFP_Fw
                     /note="color: black; sequence: 
                     CTCCTCAgtttctaagggtgaagaattgttcaccg"
     primer_bind     complement(2699..2747)
                     /label=SolGFP_Rva
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaatcaCTTATACAGCTCATCCATTCCCAGgg"
     primer_bind     complement(2699..2724)
                     /label=sGFPFc_Rv
                     /note="color: black; sequence: 
                     ttgggctcCTTATACAGCTCATCCATTCCCAGGG"
     primer_bind     complement(2701..2747)
                     /label=SolGFP_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaatcaCTTATACAGCTCATCCATTCCCAG"
     misc_feature    2740
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     primer_bind     2781..2820
                     /label=PGKpuro_Fw
                     /note="color: black; sequence: 
                     actctagagtcgacctgcaggTCTACCGGGTAGGGGAGGc"
     promoter        2802..3316
                     /label=PGK promoter
                     /note="color: #0080ff; direction: RIGHT"
     primer_bind     complement(2843..2866)
                     /label=QB0056
                     /note="color: black; sequence: CAGCGGGGCTGCTAAAGCGCATGC"
     CDS             3317..3913
                     /label=puroR
                     /note="color: #004080; direction: RIGHT"
     primer_bind     complement(3897..3934)
                     /label=PGKpuro_Rv
                     /note="color: black; sequence: 
                     tcaagcttgcatgcctgcaggGGCACCGGGCTTGCggg"
     CDS             3920..3923
                     /label=Spacer(1)
                     /note="color: #800080; direction: RIGHT"
     misc_feature    3941..3963
                     /label=1167_seq
                     /note="gene=""**"" - vntifkey=""27"" - label=""1167_seq"" -
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2158..2180"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3950..4541
                     /label=WPRE
                     /note="gene=""wPRE"" - vntifkey=""9"" - label=""WPRE"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2167..2758"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3951..4539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    3951..4539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    3951..4539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(4004..4024)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(4422..4433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4421"
                     /translation="IEGR"
     CDS             complement(4422..4433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4421"
                     /translation="IEGR"
     CDS             complement(4422..4433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4421"
                     /translation="IEGR"
     LTR             4602..4835
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4602..4835
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4602..4835
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(4860..4878)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(4860..4878)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(4860..4878)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        5667..5771
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5667..5771
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5667..5771
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5772..6632
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5772 .. 5840 / #ccffcc / signal sequence
                        2: 5841 .. 6632 / #ccffcc
                     Cleavage site after base 5840"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5772..6632
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5772 .. 5840 / #ccffcc / signal sequence
                        2: 5841 .. 6632 / #ccffcc
                     Cleavage site after base 5840"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5772..6632
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5772 .. 5840 / #ccffcc / signal sequence
                        2: 5841 .. 6632 / #ccffcc
                     Cleavage site after base 5840"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            5772..6632
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     rep_origin      6803..7391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      6803..7391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      6803..7391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        7637..7966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7637..7966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7637..7966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7817..7952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      7817..7952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      7817..7952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          9100..9165
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          9100..9165
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          9100..9165
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             9295..9315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             9295..9315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             9295..9315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    9740..9874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    9740..9874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    9740..9874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             10043..10676
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             10043..10676
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             10043..10676
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10723..10848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10723..10848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10723..10848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    join(11340..11509,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(11340..11509,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(11340..11509,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg
      781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      961 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     1021 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     1081 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     1141 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     1201 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     1261 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1321 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1381 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1441 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1501 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1561 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1621 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1681 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1741 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1801 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1861 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1921 catttcaggt gtcgtgagaa ttctcacgcg gccgccacca tgggcgttaa agtccttttc
     1981 gccctgatct gcattgccgt cgcagaggcc gtttctaagg gtgaagaatt gttcaccgga
     2041 gttgtgccca tcctggttga gctggacgga gacgttaacg gacacaagtt ctcagtgagc
     2101 ggcgaaggag agggcgacgc tacgtacggt aaactgacgt tgaagttcat ctgtacaaca
     2161 ggaaagctgc cagtgccctg gcctacactc gtgaccaccc tcacctacgg agtccagtgc
     2221 ttctcccggt accccgacca catgaaacag cacgatttct tcaagtccgc gatgcctgag
     2281 ggctatgttc aggaacgcac catattcttc aaggacgacg gtaactataa aaccagggct
     2341 gaagttaagt tcgaggggga cacgttggta aatagaattg agttgaaagg aatcgatttc
     2401 aaagaagacg gcaatatttt gggccacaag ctggagtaca actacaacag ccacaacgtg
     2461 tatattatgg ctgataagca gaagaacggc atcaaggtga acttcaaaat ccgacacaat
     2521 atcgaggatg gctctgtgca actggccgac cattaccagc aaaacacgcc tatcggcgac
     2581 ggtccggttt tgctgccaga taaccactac ctctccacgc aatcagccct ctccaaggat
     2641 cctaacgaaa aaagagacca tatggttctg cttgaattcg tgacggctgc tgggatcacc
     2701 ctgggaatgg atgagctgta taagtgatta attaaggccg caactcccac ctgcaacatg
     2761 cgtgactgac tgaggccgcg actctagagt cgacctgcag gtctaccggg taggggaggc
     2821 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     2881 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     2941 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     3001 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgac tcactagtct
     3061 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     3121 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     3181 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     3241 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     3301 cctttcgtcc gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3361 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3421 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     3481 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     3541 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     3601 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     3661 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtgtcgc ccgaccacca
     3721 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     3781 ggtgcccgcc ttcctggaga catccgcgcc ccgcaacctc cccttctacg agcggctcgg
     3841 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     3901 caagcccggt gcccctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     3961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4321 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4501 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgaccaaggc
     4561 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4621 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     4681 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4741 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4801 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     4861 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     4921 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     4981 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     5041 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     5101 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     5161 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     5221 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     5281 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     5341 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     5401 tacaatctag ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5461 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5521 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5581 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5641 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5701 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5761 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5821 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5881 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5941 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6001 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6061 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6121 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6181 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6241 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6301 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6361 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6421 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6481 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6541 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6601 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6661 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6721 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7441 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7501 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7561 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7621 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7801 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7861 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7921 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     7981 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     8041 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     8101 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     8161 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     8221 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     8281 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     8341 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     8401 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     8461 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     8521 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     8581 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     8641 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     8701 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     8761 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     8821 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     8881 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     8941 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9001 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9061 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     9121 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     9181 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     9241 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     9301 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     9361 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     9421 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     9481 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     9541 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     9601 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     9661 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     9721 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     9781 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9841 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9901 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
     9961 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10021 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10081 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10141 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    10201 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    10261 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    10321 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    10381 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    10441 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10681 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    10741 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    10801 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    10861 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    10921 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    10981 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11041 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11101 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    11161 aaaagtaaga ccaccgcaca gcaagcggcc ggtgatcttc agacctggac gatatatatg
    11221 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    11281 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    11341 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    11401 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    11461 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctggg
//
