LOCUS       Exported               10464 bp ds-DNA     circular SYN 03-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Satoshi Toda
  TITLE     Direct Submission
  JOURNAL   Exported Feb 3, 2020 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    550..729
                     /label=cPPT
                     /note="gene=""cPPT"" - vntifkey=""34"" - label=""cPPT"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     550..729"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     609..630
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="vntifkey=""27"" - label=""cPPT_5'seq"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     705..728"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     738..772
                     /label=EcoEF1a_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattccgtgaggctccggtgcccg"
     promoter        754..1937
                     /label=EF-1alpha
                     /note="vntifkey=""29"" - label=EF-1alpha - 
                     ApEinfo_fwdcolor=#ff8040 - ApEinfo_revcolor=green - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 234..1417"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     1321..1340
                     /label=QB4469
                     /note="color: black; sequence: GCCAAGATCTGCACACTGGT"
     primer_bind     1444..1462
                     /label=QB1511
                     /note="color: black; sequence: GGTAGTCTCAAGCTGGCCG"
     primer_bind     1885..1905
                     /label=EF1a forward primer binding (QB0019)
                     /note="label=EF1a forward primer binding (QB0019) - 
                     ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=cyan - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1365..1385"
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(1923..1950)
                     /label=EcoEF1a_Rv
                     /note="color: black; sequence: 
                     tgacgcgtgagaattctcacgacacctgaaa"
     primer_bind     complement(1928..1950)
                     /label=pHR_EcoRIhomo_EF1a_rev
                     /note="color: black; sequence: 
                     TTGacgcgtgagaattCGCTAGCctcacgacacc"
     primer_bind     1939..1975
                     /label=GssmCherry_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgccACCATGGGCgttaaagtcc"
     primer_bind     1939..1965
                     /label=TREmCPNE_Fw
                     /note="color: black; sequence: 
                     tgcaattctcacgcggccgccACCATGGGC"
     misc_feature    1951..1959
                     /label=KOZAK
                     /note="color: #a6acb3"
     regulatory      1954..1963
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    1960..2010
                     /label=signal sequence (Gaussia Luciferase)
                     /note="color: #000000"
     primer_bind     2009..2029
                     /label=2mCherry_Fw
                     /note="color: black; sequence: 
                     cacagttggatccgtgagcaagggcgaggagg"
     primer_bind     2009..2029
                     /label=16mCherry_Fw
                     /note="color: black; sequence: 
                     AGTGAGCggatccgtgagcaagggcgaggagg"
     primer_bind     2009..2029
                     /label=17mCherry_Fw
                     /note="color: black; sequence: 
                     cgtttccggatccgtgagcaagggcgaggagg"
     CDS             2011..2715
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2089..2109)
                     /label=QB4520
                     /note="color: black; sequence: GATCTCGAACTCGTGGCCGTT"
     primer_bind     complement(2210..2229)
                     /label=QB3350
                     /note="color: black; sequence: GGAGCCGTACATGAACTGAG"
     primer_bind     2507..2526
                     /label=QB3643
                     /note="color: black; sequence: AGCAGAGGCTGAAGCTGAAG"
     primer_bind     2551..2569
                     /label=QB5291
                     /note="color: black; sequence: gtcaagaccacctacaagg"
     primer_bind     2613..2632
                     /label=QB1634_fail
                     /note="color: black; sequence: CAAGTTGGACATCACCTCCC"
     primer_bind     complement(2692..2716)
                     /label=GssmCherry_Rv
                     /note="color: black; sequence: 
                     tcaagcttgcatgcctgcaggtcaCTTGTACAGCTCGTCCATtCCaCC"
     primer_bind     complement(2692..2716)
                     /label=17mCherry_Rv
                     /note="color: black; sequence: 
                     tcaagcttgcatgcctgcaggctacttgtacagctcgtccattccacc"
     primer_bind     complement(2693..2715)
                     /label=GssmCIgG1_Rv
                     /note="color: black; sequence: 
                     ttacaaccCTTGTACAGCTCGTCCATtCCac"
     misc_feature    2716..2757
                     /label=EAAAK linker
                     /note="color: #a6acb3"
     misc_feature    2758..2799
                     /label=PNE peptide
                     /note="color: #00ff00"
     primer_bind     complement(2779..2822)
                     /label=mCPNE_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccttaattaaTCACAACTTCTTCAGCCGGGcgac"
     primer_bind     complement(2779..2799)
                     /label=TREmCPNE_Rv
                     /note="color: black; sequence: 
                     ccgctggcCAACTTCTTCAGCCGGGcgac"
     misc_feature    2815
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     misc_feature    2896..2918
                     /label=1167_seq
                     /note="gene=""**"" - vntifkey=""27"" - label=""1167_seq"" -
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2158..2180"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     complement(2902..2923)
                     /label=QB2745
                     /note="color: black; sequence: GTAATCCAGAGGTTGATTATCG"
     misc_feature    2905..3496
                     /label=WPRE
                     /note="gene=""wPRE"" - vntifkey=""9"" - label=""WPRE"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2167..2758"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    2906..3494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    2906..3494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    2906..3494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(2959..2979)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(3377..3388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3376"
                     /translation="IEGR"
     CDS             complement(3377..3388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3376"
                     /translation="IEGR"
     CDS             complement(3377..3388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3376"
                     /translation="IEGR"
     LTR             3557..3790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3557..3790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3557..3790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(3815..3833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3815..3833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3815..3833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4727..5587
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4727 .. 4795 / #ccffcc / signal sequence
                        2: 4796 .. 5587 / #ccffcc
                     Cleavage site after base 4795"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4727..5587
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4727 .. 4795 / #ccffcc / signal sequence
                        2: 4796 .. 5587 / #ccffcc
                     Cleavage site after base 4795"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4727..5587
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4727 .. 4795 / #ccffcc / signal sequence
                        2: 4796 .. 5587 / #ccffcc
                     Cleavage site after base 4795"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4727..5587
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        6592..6921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6592..6921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6592..6921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6772..6907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6772..6907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6772..6907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     6981..6991
                     /label=GFc_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaatcatttacccggagacagggagaggc"
     intron          8055..8120
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8055..8120
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8055..8120
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             8250..8270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8250..8270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8250..8270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8695..8829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8695..8829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8695..8829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             8998..9631
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             8998..9631
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             8998..9631
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    9678..9803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    9678..9803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    9678..9803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    join(10295..10464,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10295..10464,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10295..10464,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg
      781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      961 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     1021 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     1081 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     1141 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     1201 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     1261 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1321 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1381 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1441 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1501 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1561 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1621 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1681 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1741 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1801 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1861 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1921 catttcaggt gtcgtgagaa ttctcacgcg gccgccacca tgggcgttaa agtccttttc
     1981 gccctgatct gcattgccgt cgcagaggcc gtgagcaagg gcgaggagga taacatggcc
     2041 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2101 ttcgagatcg agggtgaggg agagggtcgc ccatacgagg gcacccagac cgccaagctg
     2161 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2221 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2281 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2341 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2401 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2461 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2521 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2581 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2641 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggtggaatg
     2701 gacgagctgt acaagttggt tggagaagcg gctgccaagg aggccgctgc aaaggctaat
     2761 taccatctcg aaaatgaggt cgcccggctg aagaagttgt gattaattaa ggccgcaact
     2821 cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc tgcaggcatg
     2881 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2941 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3001 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3061 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3121 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3181 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3241 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3301 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3361 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3421 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3481 ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag ccacttttta
     3541 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3601 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3781 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     3841 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     3901 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     3961 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     4021 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     4081 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     4141 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     4201 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     4261 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     4321 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg atgccgcata
     4381 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4441 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4501 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4561 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5881 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6541 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     6601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6901 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     6961 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     7021 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     7081 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     7141 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     7201 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     7261 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     7321 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     7381 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     7441 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     7501 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     7561 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     7621 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     7681 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     7741 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     7801 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     7861 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     7921 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     7981 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     8041 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     8101 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     8161 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     8221 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     8281 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     8341 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     8401 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     8461 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     8521 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     8581 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8641 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     8701 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8761 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8821 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     8881 cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga
     8941 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     9001 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     9061 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     9121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     9181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     9301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     9361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     9421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     9481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
    10021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
    10081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
    10141 cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga agtgaattat
    10201 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10261 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    10321 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10381 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10441 atctgttgca actcacagtc tggg
//
