LOCUS pKRG3 8896 bp DNA circular 15-OCT-2020 FEATURES Location/Qualifiers misc_feature 1916..1966 /note="NLS" misc_feature 1967..6067 /note="hSpCas9" misc_feature 83..178 /note="AeU6" misc_feature 354..1746 /note="AePUb" misc_feature 1847..1915 /note="3X FLAG" source 1..8896 /dnas_title="pKRG3" ORIGIN 1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GGGTTGGCCG ATTCATTAAT GCAGGCAACT 61 CGTGAAAGGT AGGCGGATCA GCGAATGAAA TCGCCCATCG AGTTGATACG TCCATCCATC 121 GCTAGAACCG CGTTCGCTGT AGAAGACTAT ATAAGAGCAG AGGCAAGAGT AGTGAAATgG 181 AGACGaCGTC TCtGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC 241 AACTTGAAAA AGTGGCACCG AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT 301 AAAATAAGGC TAGTCCGTTT TTAGCGCTTG CGCCAATTCT GCAGACAAAT GGCTATCTTT 361 ACATGTAGCT TGTGCATTGA ATCCAATTAT AATTTGCCTT GGCACCAGCT GAGCCAGACA 421 AGAAAGAAAG CTTCCCAGAA GTATATCGAT TTAGAAGGGT TGACGTCACT TGCTGACTGC 481 ACTAATACAG CAAATGATGC AATTAGAATG ATTCAAGTGA AATTCCCAAA TTACTGATTT 541 TTCTCTGGAT TTGGTTATCA GATTACATTC GAAGCTAAGA TTAGCTACCG AAATTGTCGA 601 TCAAATCAGG AAATCCTTTC TCTATCGAAA AAGGCATTCG CACATCTTCC TCTCTATGCC 661 ATATACACGA AGGGTAGGTA CATTGACGTC TTTGCCAGAA GTTGAACTGC ATCGTTCAAG 721 GTACAGAATG AACGACTAAC AGACACAAGC ACGTTTTGCT GTCCATTCAG ACACAGGGAT 781 GGTACCCATA TTCGATCGAT ATAGAGCCAT CCAACCGAAC AGAGGTATAT GTATGAATGT 841 ATTGCTGAAA TTTTCTAGAA GTACAACCAC CACTACGACA GTGTCTATAA AACGCCCCTG 901 CAAAGGCGAA ACCAGCTCAA TCGAATACGT TTCCTAGTGG AGTGAACATT ACGCGGCCCA 961 AGTAAGCAGT GCCAGTGCAA GTGAAGTGAA GTCTCTAGTG AAAAAGAGTG ATCCAATTAG 1021 CCAGAGGAGA AAATTTCAGA GTGAACAAAG CTTTATTCAA AGGACAATTA CTATTAAATT 1081 GGTGAAAGTG CATTTCGGTG AAGGGAATCT TCTAGTGAAG GTAGGTAAAT TAATTGATGA 1141 AATTATAGCT ATGAGCGAAA ACTAGTTTGG TGAATGATTC CTTTGTCTTT GAATGAGCAA 1201 ACTATTTTCC AAGATGGCGA CTATTGAGCT TTGAGTGATT AGTGAAAATT TGCAACGCAG 1261 TTTCATCATC ATTGATAAAA CCCAATTGTG ATTCACAGCG ATAATCATAT TTCGTTGAAT 1321 CATCGCTACT AATTGAATTA AATTTCTAGA ATAATAAGAA TAACGTATTT GCTCCGTCAC 1381 ATATCTAAAA TAAATATTTT GATGGTTAAT TACCCATTAA GGTAATATTA ACACATATCG 1441 AGAAAAACCT TGAGGAAATC GTGAAAACTT GAAGATACGC AATTTCAAAA CTACGTAGTT 1501 CAAAGTCGAA AACAAGTTAA TTTTTCACTT AAAAGTAGGG CGTTGTTGTG ACGTCATCAC 1561 CTTCAAGTGT ATATTTTTCA CTTGGCCTGC GACTGCAAAC GCAGACAAAG CAAAACAAGT 1621 TTAAAACCTG TCGTGTCGTG CTCGAAGCCA AAGGCAATGA ATCAATATCA AATGAGAGTT 1681 TGCATTTCAC AACCAATTAC TCAAGCGTTT CCTCGTTTCT TTTTCTGCTC AACAGAGATT 1741 TCAACACCAA GCTCGATAAG CTTGTTCGAA TCTCGAGTGC GCGCTTCCGG AGGTATACAC 1801 CTAGGCGGTA CCACTGCAGT GAATTCGGAG CTCTACCGGT GCCACCATGG ACTATAAGGA 1861 CCACGACGGA GACTACAAGG ATCATGATAT TGATTACAAA GACGATGACG ATAAGATGGC 1921 CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCGACA AGAAGTACAG 1981 CATCGGCCTG GACATCGGCA CCAACTCTGT GGGCTGGGCC GTGATCACCG ACGAGTACAA 2041 GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC CGGCACAGCA TCAAGAAGAA 2101 CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC GAGGCCACCC GGCTGAAGAG 2161 AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC TGCTATCTGC AAGAGATCTT 2221 CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGACTGGAAG AGTCCTTCCT 2281 GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC GGCAACATCG TGGACGAGGT 2341 GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA AAGAAACTGG TGGACAGCAC 2401 CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG 2461 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT 2521 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT 2581 GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC AGACGGCTGG AAAATCTGAT 2641 CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGA AACCTGATTG CCCTGAGCCT 2701 GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC GAGGATGCCA AACTGCAGCT 2761 GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG GCCCAGATCG GCGACCAGTA 2821 CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC ATCCTGCTGA GCGACATCCT 2881 GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC TCTATGATCA AGAGATACGA 2941 CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC TGCCTGAGAA 3001 GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA TTGACGGCGG 3061 AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAAAAGA TGGACGGCAC 3121 CGAGGAACTG CTCGTGAAGC TGAACAGAGA GGACCTGCTG CGGAAGCAGC GGACCTTCGA 3181 CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG CACGCCATTC TGCGGCGGCA 3241 GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG ATCGAGAAGA TCCTGACCTT 3301 CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC AGCAGATTCG CCTGGATGAC 3361 CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAGGGCGC 3421 TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT AAGAACCTGC CCAACGAGAA 3481 GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC GTGTATAACG AGCTGACCAA 3541 AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC CTGAGCGGCG AGCAGAAAAA 3601 GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG ACCGTGAAGC AGCTGAAAGA 3661 GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG TGGAAGATCG 3721 GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA ATTATCAAGG ACAAGGACTT 3781 CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC GTGCTGACCC TGACACTGTT 3841 TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT GCCCACCTGT TCGACGACAA 3901 AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG GGCAGGCTGA GCCGGAAGCT 3961 GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC CTGGATTTCC TGAAGTCCGA 4021 CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC GACAGCCTGA CCTTTAAAGA 4081 GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC CTGCACGAGC ACATTGCCAA 4141 TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG ACAGTGAAGG TGGTGGACGA 4201 GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC GTGATCGAAA TGGCCAGAGA 4261 GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG AGAATGAAGC GGATCGAAGA 4321 GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC CCCGTGGAAA ACACCCAGCT 4381 GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG CGGGATATGT ACGTGGACCA 4441 GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC CATATCGTGC CTCAGAGCTT 4501 TCTGAAGGAC GACTCCATCG ACAACAAGGT GCTGACCAGA AGCGACAAGA ACCGGGGCAA 4561 GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG AAGAACTACT GGCGGCAGCT 4621 GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT CTGACCAAGG CCGAGAGAGG 4681 CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA CAGCTGGTGG AAACCCGGCA 4741 GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG AACACTAAGT ACGACGAGAA 4801 TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG TCCAAGCTGG TGTCCGATTT 4861 CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC AACTACCACC ACGCCCACGA 4921 CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA AAGTACCCTA AGCTGGAAAG 4981 CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG AAGATGATCG CCAAGAGCGA 5041 GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC AGCAACATCA TGAACTTTTT 5101 CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG CGGCCTCTGA TCGAGACAAA 5161 CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT TTTGCCACCG TGCGGAAAGT 5221 GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG GTGCAGACAG GCGGCTTCAG 5281 CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG ATCGCCAGAA AGAAGGACTG 5341 GGACCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG GCCTATTCTG TGCTGGTGGT 5401 GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC TGCTGGGGAT 5461 CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC GACTTTCTGG AAGCCAAGGG 5521 CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT AAGTACTCCC TGTTCGAGCT 5581 GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG GAAACGAACT 5641 GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC AGCCACTATG AGAAGCTGAA 5701 GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG GAACAGCACA AGCACTACCT 5761 GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA GTGATCCTGG CCGACGCTAA 5821 TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT AAGCCCATCA GAGAGCAGGC 5881 CaAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA GCCCCTGCCG CCTTCAAGTA 5941 CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC AAAGAGGTGC TGGACGCCAC 6001 CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG ATCGACCTGT CTCAGCTGGG 6061 AGGCGACAGC CCCAAGAAGA AGAGAAAGGT GGAGGCCAGC TAATAGGACC CAGCTTTCTT 6121 GTACAAAGTG GTGACGTAAG CTAGCAGGAT CTTTGTGAAG GAACCTTACT TCTGTGGTGT 6181 GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT AAGGTAAATA TAAAATTTTT 6241 AAGTGTATAA TGTGTTAAAC TACTGATTCT AATTGTTTGT GTATTTTAGA TTCCAACCTA 6301 TGGAACTGAT GAATGGGAGC AGTGGTGGAA TGCCTTTAAT GAGGAAAACC TGTTTTGCTC 6361 AGAAGAAATG CCATCTAGTG ATGATGAGGC TACTGCTGAC TCTCAACATT CTACTCCTCC 6421 AAAAAAGAAG AGAAAGGTAG ttGACCCCAA GGACTTTCCT TCAGAATTGC TAAGTTTTTT 6481 GAGTCATGCT GTGTTTAGTA ATAGAACTCT TGCTTGCTTT GCTATTTACA CCACAAAGGA 6541 AAAAGCTGCA CTGCTATACA AGAAAATTAT GGAAAAATAT TCTGTAACCT TTATAAGTAG 6601 GCATAACAGT TATAATCATA ACATACTGTT TTTTCTTACT CCACACAGGC ATAGAGTGTC 6661 TGCTATTAAT AACTATGCTC AAAAATTGTG TACCTTTAGC TTTTTAATTT GTAAAGGGGT 6721 TAATAAGGAA TATTTGATGT ATAGTGCCTT GACTAGAGAT CATAATCAGC CATACCACAT 6781 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA 6841 AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 6901 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 6961 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCC GTTTAAACTA CGCGTAATTC 7021 AAACAGGGTT CTGGCGTCGT TCTCGTACTG TTTTCCCCAG GCCAGTGCTT TAGCGTTATT 7081 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 7141 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 7201 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 7261 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG 7321 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 7381 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 7441 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 7501 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 7561 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 7621 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 7681 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 7741 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 7801 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 7861 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 7921 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 7981 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 8041 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 8101 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 8161 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 8221 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG 8281 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 8341 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 8401 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 8461 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT 8521 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 8581 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 8641 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 8701 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 8761 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 8821 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 8881 GCGAGGAagc ggAAGA //