LOCUS       pSL1180_HR_Aag2A        7782 bp    DNA     linear       21-OCT-2020
FEATURES             Location/Qualifiers
     misc_feature    236..1378
                     /note="Aag2 HA"
     misc_feature    3642..3644
                     /note="start"
     misc_feature    3645..3707
                     /note="3XFLAG"
     misc_feature    3784..4801
                     /note="Aag2 HA"
     misc_feature    1379..1412
                     /note="loxp"
     misc_feature    1413..2805
                     /note="AePUb"
     misc_feature    2905..3600
                     /note="RFP"
     misc_feature    3608..3641
                     /note="loxp"
     source          1..7782
                     /dnas_title="pSL1180_HR_Aag2Ago1NFLAG_loxpRFP"
ORIGIN      
        1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
       61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
      121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
      181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGACAT
      241 GTAGGACATG TGGGGTAGTT CCAGAAATCC AGATAAACGA TGGACATTTG AATTATCCCC
      301 AAACCTCCAT CATGTTCTGT TACTTGATAT CAACTCAAGG AACCCCGCAC TAAAATTGAA
      361 TTTCATGGCT ACTATGATAA CCGTGTTAAA CCGTTTTCTA AAAGTTTCTG TCGCATGACT
      421 CGTTACAAAT GACGTTACAA ATCTATAATT TCTATAAATC CCTTCAAAAT TGACTCTTGG
      481 AAATTTATCT GTATTAAAAT TATATAATTA TTTTAAAAGC TGGTAAATCA CCAACATTTT
      541 GACATCCATT TCATCCAGAA TCGTTCTATT GTTTATGCGG TGAATGCCGA AAAAAATATA
      601 CCTTCAATGA TTCATTGCCG ATGTTTCAAC TGAACTCAAA CTCAGAAATA TGAAATTTCA
      661 CGCATTTAGA TAGATGACTA CTCTTTTTTC AGAGTTTTAG TTCACAGCAA AGATAAAAGG
      721 TAATTCAAAT GAGAGCTGCT GTAGAAAGTT TCTCGCTCAA TGTATGATCT CGCCTTAAAG
      781 CCATTGGTAA CCAAGTGTGT AGCTTGTTGT TACTACGCAA ACGAATGGAT TTACACGTTA
      841 CTTAAAAATG CTACAATGAA GATTCTCTAT CTCCCAGTGG CGATAGTTAT ATTTTGGTCC
      901 ATTCATTTGC TTAAAAACAA CGAGCTACAC ATCAATGGCT TTTAGATCGA GATCACAATT
      961 TATGCGAGAT TTCCTCAAGT AATTTATTGC CAAGAAATTT CGTGAATAAT AATAATTATA
     1021 ATGATTATTC TGTTTCATGA GCGCATCCAT TTTGGAGCGT CCGTAAAATG AAACAAAACG
     1081 GTAAACCGCA ACCTGGCTGT TTACTTGCAG CGGTTTTTCG GTACCTAAAC TTGCGTACAA
     1141 GTCGAACTCG TCTAAAACAT AACGCCTACC CAGGCTGCTG GTAGCGAAAC GTTGGGGGGC
     1201 GATGAAGAAA ACTTGTCTCA CTCGCCATCA TACGAGAGTT TCGCGACGAG TTCAGCAGAC
     1261 GTGCGTGTTT AGCGCGACGA CGAAAAGTGC AAAAATTCGG CCGTAATTAG TTCGCTTCCG
     1321 TTTTTCCGAG TGCCTCCGAT CGTTAGCGGA CCGTTCCGGG TGTGATTTAA GCGTGAAAAT
     1381 AACTTCGTAT AATGTATGCT ATACGAAGTT ATTATCTTTA CATGTAGCTT GTGCATTGAA
     1441 TCCAATTATA ATTTGCCTTG GCACCAGCTG AGCCAGACAA GAAAGAAAGC TTCCCAGAAG
     1501 TATATCGATT TAGAAGGGTT GACGTCACTT GCTGACTGCA CTAATACAGC AAATGATGCA
     1561 ATTAGAATGA TTCAAGTGAA ATTCCCAAAT TACTGATTTT TCTCTGGATT TGGTTATCAG
     1621 ATTACATTCG AAGCTAAGAT TAGCTACCGA AATTGTCGAT CAAATCAGGA AATCCTTTCT
     1681 CTATCGAAAA AGGCATTCGC ACATCTTCCT CTCTATGCCA TATACACGAA GGGTAGGTAC
     1741 ATTGACGTCT TTGCCAGAAG TTGAACTGCA TCGTTCAAGG TACAGAATGA ACGACTAACA
     1801 GACACAAGCA CGTTTTGCTG TCCATTCAGA CACAGGGATG GTACCCATAT TCGATCGATA
     1861 TAGAGCCATC CAACCGAACA GAGGTATATG TATGAATGTA TTGCTGAAAT TTTCTAGAAG
     1921 TACAACCACC ACTACGACAG TGTCTATAAA ACGCCCCTGC AAAGGCGAAA CCAGCTCAAT
     1981 CGAATACGTT TCCTAGTGGA GTGAACATTA CGCGGCCCAA GTAAGCAGTG CCAGTGCAAG
     2041 TGAAGTGAAG TCTCTAGTGA AAAAGAGTGA TCCAATTAGC CAGAGGAGAA AATTTCAGAG
     2101 TGAACAAAGC TTTATTCAAA GGACAATTAC TATTAAATTG GTGAAAGTGC ATTTCGGTGA
     2161 AGGGAATCTT CTAGTGAAGG TAGGTAAATT AATTGATGAA ATTATAGCTA TGAGCGAAAA
     2221 CTAGTTTGGT GAATGATTCC TTTGTCTTTG AATGAGCAAA CTATTTTCCA AGATGGCGAC
     2281 TATTGAGCTT TGAGTGATTA GTGAAAATTT GCAACGCAGT TTCATCATCA TTGATAAAAC
     2341 CCAATTGTGA TTCACAGCGA TAATCATATT TCGTTGAATC ATCGCTACTA ATTGAATTAA
     2401 ATTTCTAGAA TAATAAGAAT AACGTATTTG CTCCGTCACA TATCTAAAAT AAATATTTTG
     2461 ATGGTTAATT ACCCATTAAG GTAATATTAA CACATATCGA GAAAAACCTT GAGGAAATCG
     2521 TGAAAACTTG AAGATACGCA ATTTCAAAAC TACGTAGTTC AAAGTCGAAA ACAAGTTAAT
     2581 TTTTCACTTA AAAGTAGGGC GTTGTTGTGA CGTCATCACC TTCAAGTGTA TATTTTTCAC
     2641 TTGGCCTGCG ACTGCAAACG CAGACAAAGC AAAACAAGTT TAAAACCTGT CGTGTCGTGC
     2701 TCGAAGCCAA AGGCAATGAA TCAATATCAA ATGAGAGTTT GCATTTCACA ACCAATTACT
     2761 CAAGCGTTTC CTCGTTTCTT TTTCTGCTCA ACAGAGATTT CAACACCAAG CTCGATAAGC
     2821 TTGTTCGAAT CTCGAGTGCG CGCTTCCGGA GGTATACACC TAGGCGGTAC CACTGCAGTG
     2881 AATTCGGAGC TCTACCGCGG CCGCATGAGC GAGCTGATCA AGGAGAACAT GCACATGAAG
     2941 CTGTACATGG AGGGCACCGT GAACAACCAC CACTTCAAGT GCACATCCGA GGGCGAAGGC
     3001 AAGCCCTACG AGGGCACCCA GACCATGAAG ATCAAGGTGG TCGAGGGCGG CCCTCTCCCC
     3061 TTCGCCTTCG ACATCCTGGC TACCAGCTTC ATGTACGGCA GCAAAGCCTT CATCAACCAC
     3121 ACCCAGGGCA TCCCCGACTT CTTTAAGCAG TCCTTCCCTG AGGGCTTCAC ATGGGAGAGA
     3181 ATCACCACAT ACGAAGACGG GGGCGTGCTG ACCGCTACCC AGGACACCAG CTTCCAGAAC
     3241 GGCTGCATCA TCTACAACGT CAAGATCAAC GGGGTGAACT TCCCATCCAA CGGCCCTGTG
     3301 ATGCAGAAGA AAACACGCGG CTGGGAGGCC AACACCGAGA TGCTGTACCC CGCTGACGGC
     3361 GGCCTGAGAG GCCACAGCCA GATGGCCCTG AAGCTCGTGG GCGGGGGCTA CCTGCACTGC
     3421 TCCTTCAAGA CCACATACAG ATCCAAGAAA CCCGCTAAGA ACCTCAAGAT GCCCGGCTTC
     3481 CACTTCGTGG ACCACAGACT GGAAAGAATC AAGGAGGCCG ACAAAGAGAC CTACGTCGAG
     3541 CAGCACGAGA TGGCTGTGGC CAAGTACTGC GACCTCCCTA GCAAACTGGG GCACAGATAA
     3601 TTAATTAATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TATGGATTAC AAGGATCACG
     3661 ATGGAGATTA CAAGGATCAC GATATCGATT ACAAGGATGA TGATAAGTAT CCGGTGGGTC
     3721 AACGTAAGTA CCGTTTCCGG ACGTTTGAAA AAAGTGCAAT TCACAAGAGA AGAAAAAAAA
     3781 AaGTGTTGgC GAGGGGACCT TCCTTCCTCC CTCTCGTCTC TCTCGCTGTG TAAATATTTC
     3841 TTCCCCCATG TGTGTAATTT TCCTGGCCTG CTGCTCTGTT TGGTGCTTCT CTGGACGTTT
     3901 TTGTGCGTGT GcGTTGGTGT GATGGGGGGC TTTTCCTGTG TACGGACGAA GAACTTTGTG
     3961 TCGGTGTGCG TGTGAGATGA GGCCCTCGCC AGAATGAAAA TAAACCTCAT CAACAATGAA
     4021 AAGTGTCACC ACTTTATGGA ATAAAAAACG TTTTTATGCA GAAGAGCATG TGAATTATGT
     4081 ATCACTTGGC GATGGAATTG GGAACATTGA TGCTTCCGGA CCGATGGAAT AATGGAAAAC
     4141 CAGATGTTTC TGCACGTTCT TTGGCACCTT GGCAATGTGT ACTAATCAAC ACCTCCCTCG
     4201 AAAAATATAC TTGGTGATCA GAATGTTTGT GTTTGTGAAT AGTAGGTAGT TTCCGTGTGT
     4261 GGGTGTGTGT CTTTGCTAAT CATCCTGCTC CAGAAAAAAA GGAAAACCAC CAATTTTAAT
     4321 CATCATTTTC TGCGGCTTCG GAGACTGATA AGAGGAATAC AACTGTTTGC AAACAGTGGA
     4381 TTGAAAACAA TGGATAGAAG TTTTATTGGT TCACTTCAGT TCTAAACAGC GGATCCATCT
     4441 GAAAAGTTAT ATGAAATTGT ACCCCCTTTT CAGTGCTGGG AAATTATTGC CTTTTGTGTC
     4501 GCTCTTTGCT CACAACTACA TACGGAGATG CAGACAGAGC TCGCATTATG TTGGTGGTGC
     4561 TCTGTCGCTG GCCATCCGAG AATCGGAAAC ATCGTGCGGA TGGAACATGG AAGAAGAGGA
     4621 CGTTGTAGGG TTTACCATTA TGTATATGCG TTCAGGCGAC ATCAGACGAG GTGGGTCTTC
     4681 TCGAAATGAT GGGTGGTTGA CTGAGTAGCA CCTGCTGTTC ATGTGCGGAA ATGGAATAAC
     4741 GTGGAGATGC AATGTTCTTC AACGCTAGAA GGATTCGGGG AGACGAAAAG CAAATCCGGT
     4801 GGCGGCCGCC TGCAGCTGGC GCCATCGATA CGCGTACGTC GCGACCGCGG ACATGTACAG
     4861 AGCTCGAGAA GTACTAGTGG CCACGTGGGC CGTGCACCTT AAGCTTGGCA CTGGCCGTCG
     4921 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC
     4981 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC
     5041 AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT
     5101 GCGGTATTTC ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT
     5161 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
     5221 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
     5281 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
     5341 CAAAAAACTT GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT
     5401 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
     5461 AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
     5521 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
     5581 AACGTTTACA ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
     5641 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
     5701 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC
     5761 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
     5821 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     5881 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     5941 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     6001 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     6061 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     6121 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     6181 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     6241 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     6301 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT
     6361 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
     6421 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT
     6481 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC
     6541 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
     6601 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
     6661 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT
     6721 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
     6781 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA
     6841 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     6901 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     6961 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     7021 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     7081 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     7141 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     7201 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     7261 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     7321 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     7381 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     7441 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     7501 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     7561 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     7621 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     7681 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG
     7741 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GA
//

