LOCUS       Exported               10554 bp ds-DNA     circular SYN 26-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 26, 2020 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    550..729
                     /label=cPPT
                     /note="gene=""cPPT"" - vntifkey=""34"" - label=""cPPT"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     550..729"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     609..630
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="vntifkey=""27"" - label=""cPPT_5'seq"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     705..728"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     738..772
                     /label=EcoEF1a_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattccgtgaggctccggtgcccg"
     promoter        754..1937
                     /label=EF-1alpha
                     /note="vntifkey=""29"" - label=EF-1alpha - 
                     ApEinfo_fwdcolor=#ff8040 - ApEinfo_revcolor=green - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 234..1417"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     1321..1340
                     /label=QB4469
                     /note="color: black; sequence: GCCAAGATCTGCACACTGGT"
     primer_bind     1444..1462
                     /label=QB1511
                     /note="color: black; sequence: GGTAGTCTCAAGCTGGCCG"
     primer_bind     1885..1905
                     /label=EF1a forward primer binding (QB0019)
                     /note="label=EF1a forward primer binding (QB0019) - 
                     ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=cyan - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1365..1385"
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(1923..1950)
                     /label=EcoEF1a_Rv
                     /note="color: black; sequence: 
                     tgacgcgtgagaattctcacgacacctgaaa"
     primer_bind     complement(1928..1950)
                     /label=pHR_EcoRIhomo_EF1a_rev
                     /note="color: black; sequence: 
                     TTGacgcgtgagaattCGCTAGCctcacgacacc"
     primer_bind     1939..1973
                     /label=EFGFPlig_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgcCACCATGGAGACCGACAC"
     primer_bind     1939..1967
                     /label=GFPlig_Fw
                     /note="color: black; sequence: 
                     tgcaattctcacgcgtGCCGCCACCATGGAGAC"
     misc_feature    1951..1959
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             1960..2892
                     /codon_start=1
                     /note="color: #000000"
                     /translation="METDTLLLWVLLLWVPGSTGDMVSKGEELFTGVVPILVELDGDVN
                     GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHD
                     FFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKL
                     EYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY
                     LSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKNAVGQDTQEVIVVPHSLPFKV
                     VVISAILALVVLTIISLIILIMLWQKKPR"
     misc_feature    1960..2022
                     /label=signal sequence (Ig kappa chain V-III region mouse
                     /note="color: #ff0000"
     misc_feature    2023..2739
                     /label=EGFP
                     /note="color: #00ff00"
     primer_bind     2026..2053
                     /label=HGFP_Fw
                     /note="color: black; sequence: 
                     cggcggcGTTTCTAAGGGTGAAGAATTGTTCACCG"
     primer_bind     complement(2493..2512)
                     /label=QB2643
                     /note="color: black; sequence: CCTTGATGCCGTTCTTCTGC"
     primer_bind     complement(2715..2739)
                     /label=Itgb2_GFP_Rv
                     /note="color: black; sequence: 
                     tcaagcttgcatgcctgcaggctacttatacagctcatccattcccagg"
     primer_bind     complement(2715..2739)
                     /label=HGFP_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaactaCTTATACAGCTCATCCATTCCCAGG"
     misc_feature    2806..2874
                     /label=transmembrane
                     /note="color: #99ccff"
     primer_bind     complement(2869..2915)
                     /label=GFPlig_Rv
                     /note="color: black; sequence: 
                     GTGggagttgcggccgcTTATCGGGGTTTTTTTTGCCACAG"
     primer_bind     complement(2874..2912)
                     /label=EFGFPlig_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaaTTATCGGGGTTTTTTTTGC"
     misc_feature    2905
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     misc_feature    2986..3008
                     /label=1167_seq
                     /note="gene=""**"" - vntifkey=""27"" - label=""1167_seq"" -
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2158..2180"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    2995..3586
                     /label=WPRE
                     /note="gene=""wPRE"" - vntifkey=""9"" - label=""WPRE"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2167..2758"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    2996..3584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    2996..3584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    2996..3584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(3049..3069)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(3467..3478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3466"
                     /translation="IEGR"
     CDS             complement(3467..3478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3466"
                     /translation="IEGR"
     CDS             complement(3467..3478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3466"
                     /translation="IEGR"
     LTR             3647..3880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3647..3880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3647..3880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(3905..3923)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3905..3923)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3905..3923)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4712..4816
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4712..4816
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4712..4816
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4817..5677
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4817 .. 4885 / #ccffcc / signal sequence
                        2: 4886 .. 5677 / #ccffcc
                     Cleavage site after base 4885"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4817..5677
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4817 .. 4885 / #ccffcc / signal sequence
                        2: 4886 .. 5677 / #ccffcc
                     Cleavage site after base 4885"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4817..5677
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4817 .. 4885 / #ccffcc / signal sequence
                        2: 4886 .. 5677 / #ccffcc
                     Cleavage site after base 4885"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4817..5677
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     rep_origin      5848..6436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5848..6436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5848..6436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        6682..7011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6682..7011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6682..7011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6862..6997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6862..6997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6862..6997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          8145..8210
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8145..8210
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8145..8210
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             8340..8360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8340..8360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8340..8360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8785..8919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8785..8919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8785..8919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             9088..9721
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             9088..9721
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             9088..9721
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    9768..9893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    9768..9893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    9768..9893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    join(10385..10554,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10385..10554,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10385..10554,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg
      781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      961 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     1021 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     1081 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     1141 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     1201 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     1261 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1321 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1381 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1441 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1501 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1561 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1621 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1681 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1741 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1801 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1861 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1921 catttcaggt gtcgtgagaa ttctcacgcg gccgccacca tggagaccga caccctcttg
     1981 ctctgggtcc tgctgctgtg ggttccgggt agtaccggag acatggtttc taagggtgaa
     2041 gaattgttca ccggagttgt gcccatcctg gttgagctgg acggagacgt taacggacac
     2101 aagttctcag tgagcggcga aggagagggc gacgctacgt acggtaaact gacgttgaag
     2161 ttcatctgta caacaggaaa gctgccagtg ccctggccta cactcgtgac caccctcacc
     2221 tacggagtcc agtgcttctc ccggtacccc gaccacatga aacagcacga tttcttcaag
     2281 tccgcgatgc ctgagggcta tgttcaggaa cgcaccatat tcttcaagga cgacggtaac
     2341 tataaaacca gggctgaagt taagttcgag ggggacacgt tggtaaatag aattgagttg
     2401 aaaggaatcg atttcaaaga agacggcaat attttgggcc acaagctgga gtacaactac
     2461 aacagccaca acgtgtatat tatggctgat aagcagaaga acggcatcaa ggtgaacttc
     2521 aaaatccgac acaatatcga ggatggctct gtgcaactgg ccgaccatta ccagcaaaac
     2581 acgcctatcg gcgacggtcc ggttttgctg ccagataacc actacctctc cacgcaatca
     2641 gccctctcca aggatcctaa cgaaaaaaga gaccatatgg ttctgcttga attcgtgacg
     2701 gctgctggga tcaccctggg aatggatgag ctgtataaga acgccgtggg ccaggatacg
     2761 caggaggtga tcgtcgtgcc ccacagcttg cccttcaagg tcgtggttat tagtgctatc
     2821 ctggcgttgg tggtgctgac tattatctcc cttatcatac ttatcatgct gtggcaaaaa
     2881 aaaccccgat aattaattaa ggccgcaact cccacctgca acatgcgtga ctgactgagg
     2941 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     3001 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3061 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3121 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3181 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3241 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3301 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3361 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3421 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3481 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3541 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     3601 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3661 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3721 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3781 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3841 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     3901 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     3961 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     4021 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     4081 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     4141 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     4201 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     4261 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     4321 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     4381 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     4441 ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4501 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4561 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4621 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4681 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6601 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6661 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6781 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6841 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6901 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6961 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     7021 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     7081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     7141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     7201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     7261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     7321 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     7381 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     7441 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     7501 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     7561 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     7621 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     7681 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     7741 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     7801 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     7861 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     7921 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     7981 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     8041 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     8101 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     8161 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     8221 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     8281 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     8341 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     8401 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     8461 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     8521 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     8581 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     8641 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     8701 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8761 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8881 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8941 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt
     9001 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     9061 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     9121 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     9181 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     9241 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     9301 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     9361 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     9421 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     9481 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     9541 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9601 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9661 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9721 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9781 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9841 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9901 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9961 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    10021 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    10081 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    10141 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    10201 aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc tggacgatat
    10261 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    10321 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    10381 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    10441 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    10501 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggg
//
