LOCUS       Exported               10593 bp ds-DNA     circular SYN 17-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Satoshi Toda
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 29, 2020 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    550..729
                     /label=cPPT
                     /note="gene=""cPPT"" - vntifkey=""34"" - label=""cPPT"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     550..729"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     609..630
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="vntifkey=""27"" - label=""cPPT_5'seq"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     705..728"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     738..772
                     /label=EcoEF1a_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattccgtgaggctccggtgcccg"
     promoter        754..1937
                     /label=EF-1alpha
                     /note="vntifkey=""29"" - label=EF-1alpha - 
                     ApEinfo_fwdcolor=#ff8040 - ApEinfo_revcolor=green - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 234..1417"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     1321..1340
                     /label=QB4469
                     /note="color: black; sequence: GCCAAGATCTGCACACTGGT"
     primer_bind     1444..1462
                     /label=QB1511
                     /note="color: black; sequence: GGTAGTCTCAAGCTGGCCG"
     primer_bind     1885..1905
                     /label=EF1a forward primer binding (QB0019)
                     /note="label=EF1a forward primer binding (QB0019) - 
                     ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=cyan - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1365..1385"
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     complement(1923..1950)
                     /label=EcoEF1a_Rv
                     /note="color: black; sequence: 
                     tgacgcgtgagaattctcacgacacctgaaa"
     primer_bind     complement(1928..1950)
                     /label=pHR_EcoRIhomo_EF1a_rev
                     /note="color: black; sequence: 
                     TTGacgcgtgagaattCGCTAGCctcacgacacc"
     primer_bind     1939..1971
                     /label=HALaG17_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgcCACCATGGCATTGCCC"
     primer_bind     1939..1966
                     /label=mtLaG17_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccGCCACCATGGCAT"
     primer_bind     1939..1964
                     /label=GssHA_PNE_Fw
                     /note="color: black; sequence: aattctcacgcggccgcCACCATGGC"
     primer_bind     1951..1963
                     /label=LaG_Fw
                     /note="color: black; sequence: tggatccGCCGCCACCATGG"
     misc_feature    1951..1959
                     /label=Kozak
                     /note="color: #99ccff"
     CDS             1960..2049
                     /codon_start=1
                     /note="color: #000000"
                     /translation="MALPVTALLLPLALLLHAARPYPYDVPDYA"
     misc_feature    1960..2022
                     /label=Signal Sequence
                     /note="color: #ffff00"
     misc_feature    2023..2049
                     /label=HAtag
                     /note="color: #a6acb3"
     primer_bind     complement(2026..2057)
                     /label=GssHA_PNE_Rv
                     /note="color: black; sequence: 
                     ACGGCGTCAGCGTAATCTGGAACATCGTATGG"
     primer_bind     2043..2072
                     /label=HA52SR4_Fw
                     /note="color: black; sequence: 
                     TTACGCTGACGCCGTTGTGACCCAGgaatc"
     CDS             2050..2778
                     /codon_start=1
                     /label=C11L34
                     /note="color: #ff0000"
                     /translation="DAVVTQESALTTSPGETVTLTCRSSTGAVTTSNYASWVQEKPDHL
                     FTGLIGGTNNRAPGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYSNHWVFGGGT
                     KLTVLGGGGGSGGGGSGGGGSGGGGSDVQLQESGPGLVAPSQSLSITCTVSGFSLTDYG
                     VNWVRQSPGKGLEWLGVIWGDGITDYNSALKSRLSVTKDNSKSQVFLKMNSLQSGDSAR
                     YYCVTGLFDYWGQGTTLTVSS"
     misc_feature    2083..2085
                     /label=C11L34-->52SR4 Thr-->Ser
                     /note="color: #a6acb3"
     misc_feature    2320..2322
                     /label=C11L34-->52SR4 Ala-->Val
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2335..2337
                     /label=C11L34-->52SR4 Asn-->Asp
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    2521..2523
                     /label=C11L34-->52SR4 Ser-->Leu
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2540..2559)
                     /label=270
                     /note="color: black; sequence: GCTCTGACGAACCCAGTTCA"
     primer_bind     complement(2755..2786)
                     /label=mt52SR4_Rv
                     /note="color: black; sequence: 
                     acggcgttGGAGGAAACAGTCAGAGTTGTCCC"
     primer_bind     complement(2767..2779)
                     /label=343
                     /note="color: black; sequence: agtccaggatGGAGGAAACAGT"
     primer_bind     2772..2798
                     /label=52SR4TMD_Fw
                     /note="color: black; sequence: TTCCTCCaacgccgtgggccaggatac"
     primer_bind     2776..2791
                     /label=17TM_Fw
                     /note="color: black; sequence: CGTTTCCAACGCCGTGGGCC"
     CDS             2776..2779
                     /codon_start=1
                     /note="color: #000000"
                     /translation="S"
     misc_feature    2776..2778
                     /label=LaG_17 GFP Nanobody
                     /note="color: #ccffcc"
     primer_bind     2777..2798
                     /label=LaM4TMD_Fw
                     /note="color: black; sequence: CTTTACCaacgccgtgggccaggatac"
     CDS             2779..2931
                     /codon_start=1
                     /note="color: #000000"
                     /translation="NAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLW
                     QKKPR"
     misc_feature    2779
                     /label=NL domains through cytoplasmic
                     /note="color: #a6acb3"
     CDS             2779
                     /codon_start=1
                     /label=Notch NL domains through cytoplasmic
                     /note="color: #000000"
                     /translation=""
     misc_feature    2845..2913
                     /label=transmembrane
                     /note="color: #99ccff"
     primer_bind     complement(2908..2954)
                     /label=GFPlig_Rv
                     /note="color: black; sequence: 
                     GTGggagttgcggccgcTTATCGGGGTTTTTTTTGCCACAG"
     primer_bind     complement(2913..2951)
                     /label=LaM4TMD_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccttaattaattatcggggttttttttgc"
     primer_bind     complement(2913..2951)
                     /label=17TM_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaaTTATCGGGGTTTTTTTTGC"
     misc_feature    2944
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     misc_feature    3025..3047
                     /label=1167_seq
                     /note="gene=""**"" - vntifkey=""27"" - label=""1167_seq"" -
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2158..2180"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3034..3625
                     /label=WPRE
                     /note="gene=""wPRE"" - vntifkey=""9"" - label=""WPRE"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2167..2758"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3035..3623
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="color: #ffffff"
     misc_feature    3035..3623
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="color: #ffffff"
     misc_feature    3035..3623
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(3088..3108)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(3506..3517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3505"
                     /translation="IEGR"
     CDS             complement(3506..3517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3505"
                     /translation="IEGR"
     CDS             complement(3506..3517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3505"
                     /translation="IEGR"
     LTR             3686..3919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3686..3919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             3686..3919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(3944..3962)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3944..3962)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(3944..3962)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4751..4855
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4751..4855
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4751..4855
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     gene            4856..5716
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     CDS             4856..5716
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4856 .. 4924 / #ccffcc / signal sequence
                        2: 4925 .. 5716 / #ccffcc
                     Cleavage site after base 4924"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4856..5716
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4856 .. 4924 / #ccffcc / signal sequence
                        2: 4925 .. 5716 / #ccffcc
                     Cleavage site after base 4924"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             4856..5716
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4856 .. 4924 / #ccffcc / signal sequence
                        2: 4925 .. 5716 / #ccffcc
                     Cleavage site after base 4924"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5887..6475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5887..6475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      5887..6475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        6721..7050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6721..7050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6721..7050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          8184..8249
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8184..8249
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     intron          8184..8249
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             8379..8399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8379..8399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             8379..8399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    8824..8958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8824..8958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    8824..8958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             9127..9760
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             9127..9760
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             9127..9760
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    9807..9932
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
                     /note="color: #ffe4c4"
     misc_feature    9807..9932
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
                     /note="color: #ffe4c4"
     misc_feature    9807..9932
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
                     /note="color: #ffe4c4"
     misc_feature    join(10424..10593,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10424..10593,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    join(10424..10593,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg
      781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      961 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     1021 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     1081 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     1141 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     1201 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     1261 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1321 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1381 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1441 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1501 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1561 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1621 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1681 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1741 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1801 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1861 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1921 catttcaggt gtcgtgagaa ttctcacgcg gccgccacca tggcattgcc cgtgaccgcc
     1981 ctgctgctgc cactggcctt gttgctccac gccgcgcggc catacccata cgatgttcca
     2041 gattacgctg acgccgttgt gacccaggaa tccgctctga ccacctctcc aggcgaaacc
     2101 gtgactctga cttgccgtag tagcaccggg gctgtgacca catctaacta tgccagttgg
     2161 gtccaggaaa aaccggatca cctgtttact ggcctgattg gcggcaccaa caatcgcgca
     2221 ccgggtgtgc ccgctcgttt cagcggttcc ctgattgggg acaaggcagc actgactatc
     2281 accggcgccc agaccgaaga tgaggcgatc tatttttgcg ccctgtggta cagcaaccat
     2341 tgggtgttcg ggggaggcac caaactgaca gtgctgggcg gaggaggagg ttcaggagga
     2401 ggaggtagcg ggggaggcgg ttccggggga ggcggttctg atgtgcagct gcaagaatcc
     2461 gggccaggac tggttgcgcc ttctcagagt ctgtcaatta catgtactgt tagtggcttt
     2521 agcctgaccg actatggtgt gaactgggtt cgtcagagcc caggcaaggg tctggagtgg
     2581 ctgggagtga tttgggggga tggaatcaca gactacaata gcgcactgaa atctcggctg
     2641 agtgttacca aagataacag caagtcccag gtcttcctga agatgaacag cctgcaaagc
     2701 ggcgactccg ctcgctatta ctgcgttacc ggactgtttg attattgggg gcaggggaca
     2761 actctgactg tttcctccaa cgccgtgggc caggatacgc aggaggtgat cgtcgtgccc
     2821 cacagcttgc ccttcaaggt cgtggttatt agtgctatcc tggcgttggt ggtgctgact
     2881 attatctccc ttatcatact tatcatgctg tggcaaaaaa aaccccgata attaattaag
     2941 gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct
     3001 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3061 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3121 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3181 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3241 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3301 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3361 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3421 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3481 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3541 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3601 ctccctttgg gccgcctccc cgcatcgata ccgtcgacca aggcagctgt agatcttagc
     3661 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3721 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3901 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
     3961 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
     4021 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
     4081 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
     4141 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
     4201 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     4261 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     4321 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     4381 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     4441 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctagctctga
     4501 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4561 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4621 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4681 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
     6721 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6781 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6841 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7021 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
     7081 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     7141 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     7201 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     7261 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     7321 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
     7381 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     7441 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     7501 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     7561 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
     7621 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
     7681 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     7741 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     7801 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     7861 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     7921 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     7981 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     8041 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     8101 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     8161 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     8221 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     8281 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     8341 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     8401 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     8461 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     8521 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     8581 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     8641 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     8701 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     8761 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     8821 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8881 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8941 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9001 accccatacc ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga
     9061 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9121 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     9181 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     9241 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     9301 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     9361 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     9421 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     9481 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     9541 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     9601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     9781 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     9841 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     9901 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     9961 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    10021 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    10081 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    10141 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    10201 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    10261 cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac aattggagaa
    10321 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10381 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10441 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10501 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    10561 cgcaacagca tctgttgcaa ctcacagtct ggg
//
