LOCUS       Exported               10714 bp ds-DNA     circular SYN 14-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Satoshi Toda
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2020 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7577..7598
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     primer_bind     7706..7737
                     /label=TRE3GS_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattcccgtggaattatcacc"
     promoter        7740..8107
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
                     /note="color: #99ccff; direction: RIGHT"
     primer_bind     complement(8094..8123)
                     /label=TRE3GS_Rv
                     /note="color: black; sequence: 
                     gtgtatacgggaattctttacgagggtagg"
     primer_bind     8127..8167
                     /label=TREmCPNE_new_Fw
                     /note="color: black; sequence: 
                     tgcaattctcacgcgtgccgccACCATGggcgttaaagtcc"
     primer_bind     8127..8153
                     /label=sGFPP_Fw
                     /note="color: black; sequence: tgcaattctcacgcgtGCCGCCACCAT"
     primer_bind     8130..8167
                     /label=GssmCherry_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgccACCATGGGCgttaaagtcc"
     primer_bind     8130..8161
                     /label=TsGFP_Fw
                     /note="color: black; sequence: 
                     tcgaattctcaCGCGtGCCGCCACCATGggcgtta"
     primer_bind     8130..8157
                     /label=solGFP_Fw
                     /note="color: black; sequence: aattctcacgcgGCCGCCACCATGGGC"
     misc_feature    8143..8151
                     /label=KOZAK
                     /note="color: #a6acb3"
     misc_feature    8152..8202
                     /label=signal sequence (Gaussia Luciferase)
                     /note="color: #000000"
     primer_bind     8152..8174
                     /label=L452_Fw
                     /note="color: black; sequence: 
                     gcctcatatgggcgttaaagtccttttcgc"
     CDS             8203..8916
                     /codon_start=1
                     /note="color: #000000"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    8203..8916
                     /label=EGFP
                     /note="color: #00ff00"
     primer_bind     complement(8670..8689)
                     /label=QB2643
                     /note="color: black; sequence: CCTTGATGCCGTTCTTCTGC"
     primer_bind     8819..8846
                     /label=GmWnt3a_Fw
                     /note="color: black; sequence: 
                     CCCTCTCCAAGGATCCTAACGAAAAAAG"
     primer_bind     complement(8890..8916)
                     /label=TsGFP_Rv
                     /note="color: black; sequence: 
                     TCAAGCTTGCATGCCtgcaggtcaCTTATACAGCTCATCCATTCCCAGGGT"
     primer_bind     complement(8891..8924)
                     /label=sGFPB_Rv
                     /note="color: black; sequence: 
                     ccgctggcCTTATACAGCTCATCCATTCCCAGGG"
     primer_bind     complement(8893..8916)
                     /label=SolGFP_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaatcaCTTATACAGCTCATCCATTCCCAG"
     primer_bind     complement(8899..8916)
                     /label=mcherry_Rv
                     /note="color: black; sequence: 
                     TCAAGCTTGCATGCCtgcaggTTACTTGTACAGCTCGTCCAT"
     primer_bind     8910..8933
                     /label=sGPBFP_Fw
                     /note="color: black; sequence: GTATAAGgccagcggcagcggcgc"
     primer_bind     8915..8933
                     /label=P2A_Fw.FOR
                     /note="color: black; sequence: CGTCCAGgccagcggcagcggcgc"
     misc_feature    8915..8917
                     /label=Brn2
                     /note="color: #ff8a29"
     CDS             8915..8916
                     /codon_start=1
                     /label=mBrn2
                     /note="color: #993366"
                     /translation=""
     primer_bind     8917..8934
                     /label=GlPBFP_Fw
                     /note="color: black; sequence: ACCCCGAgccagcggcagcggcgcc"
     primer_bind     8917..8934
                     /label=P2A-mCherry_Fw.FOR Ascl1
                     /note="color: black; sequence: ctggttcgccagcggcagcggcgcc"
     primer_bind     8917..8934
                     /label=P2A(2)_Fw.FOR
                     /note="color: black; sequence: tcaggtcgccagcggcagcggcgcc"
     primer_bind     8917..8934
                     /label=P2A-mCherry_Fw.FOR Ngn2
                     /note="color: black; sequence: TTGCATCgccagcggcagcggcgcc"
     primer_bind     8917..8934
                     /label=TBP2AmC_Fw
                     /note="color: black; sequence: caatagcgccagcggcagcggcgcc"
     CDS             8932..8988
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8968..8994)
                     /label=P2A_Rv.REV
                     /note="color: black; sequence: 
                     atcagctcgctcatatgaggcccggggttttcttcaac"
     primer_bind     complement(8968..8994)
                     /label=P2A(2)_Rv.REV
                     /note="color: black; sequence: 
                     atcagctcgctcatatgaggcccggggttttcttcaac"
     primer_bind     complement(8974..9005)
                     /label=TREsGFPP2A_Rv
                     /note="color: black; sequence: 
                     tttggatccaccatatgaggcccggggttttc"
     primer_bind     complement(8974..8995)
                     /label=sGFPP2A for L452_Rv
                     /note="color: black; sequence: acgcccatatgaggcccggggttttc"
     primer_bind     complement(8975..8997)
                     /label=RhoAP2A_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggtttt"
     primer_bind     complement(8975..8997)
                     /label=DTRP2A_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggtttt"
     primer_bind     complement(8978..8997)
                     /label=sGFPP_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggt"
     primer_bind     complement(8979..9005)
                     /label=52SR4P2A_Rv
                     /note="color: black; sequence: tttggatccaccatatgaggcccgggg"
     primer_bind     complement(8979..9005)
                     /label=LaM452SR4_Rv
                     /note="color: black; sequence: tttggatccaccatatgaggcccgggg"
     primer_bind     complement(8980..8997)
                     /label=SP2A_Rv
                     /note="color: black; sequence: CCCTTGCTCACCATatgaggcccggg"
     primer_bind     8985..9024
                     /label=NLS_IFP_for_TREU.FOR
                     /note="color: black; sequence: 
                     gcctcatatggtggatccaaaaaagaagagaaaggtagat"
     primer_bind     8985..9024
                     /label=NLS_IFP2_for_TetOW.FOR
                     /note="color: black; sequence: 
                     gcctcatatggtggatccaaaaaagaagagaaaggtagat"
     misc_feature    8992..8997
                     /label=mCherry
                     /note="color: #ff0000; direction: RIGHT"
     CDS             8992..8994
                     /codon_start=1
                     /gene="TagBFP"
                     /label=TagBFP
                     /note="color: #993366"
                     /translation="M"
     misc_feature    8992
                     /label=eGFP
                     /note="color: #00ff00; direction: RIGHT"
     CDS             8992
                     /codon_start=1
                     /label=mIFP
                     /note="color: #993366"
                     /translation=""
     CDS             8992
                     /codon_start=1
                     /note="color: #993366"
                     /translation=""
     CDS             9001..9021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             9025..9044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRK"
     primer_bind     complement(9039..9065)
                     /label=NLSI_Rv
                     /note="color: black; sequence: TCCCGAGCGGAGCCGCTGCCaaccttc"
     primer_bind     complement(9042..9058)
                     /label=NLSG_Rv
                     /note="color: black; sequence: TTGCTCACGGAGCCGCTGCCaacc"
     primer_bind     complement(9042..9058)
                     /label=2xNLS_Rv
                     /note="color: black; sequence: TTGCTCACGGAGCCGCTGCCTACC"
     primer_bind     9051..9079
                     /label=NIFP_Fw
                     /note="color: black; sequence: 
                     CGGCTCCGCTCGGGACCCTCAACCTTTCT"
     CDS             9058..10020
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /note="color: #a0003c"
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     misc_feature    9058
                     /label=mCherry
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(9994..10020)
                     /label=SbfI_IFP2forTetOW.REV
                     /note="color: black; sequence: 
                     tcaagcttgcatgcctgcaggtcaggcttctttcctctgcacctgcag"
     primer_bind     complement(9999..10040)
                     /label=H2BIFP_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaaTCAGGCTTCTTTCCTCTGCacc"
     primer_bind     complement(10009..10040)
                     /label=PacI_IFP_for TREU.REV
                     /note="color: black; sequence: 
                     ggagttgcggccttaattaatcaggcttcttt"
     misc_feature    10033
                     /label=promPGK
                     /note="color: #996633; direction: RIGHT"
     primer_bind     complement(10120..10141)
                     /label=QB2745
                     /note="color: black; sequence: GTAATCCAGAGGTTGATTATCG"
     enhancer        10123..10714
                     /label=WPRE
                     /note="color: #a6acb3"
     primer_bind     complement(10177..10197)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cccgtggaat tatcacctcg
     7741 agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga
     7801 gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag tttactccct
     7861 atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac
     7921 agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga
     7981 gaacgtataa gctttgctta tgtaaaccag ggcgcctata aaagagtgct gattttttga
     8041 gtaaacttca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     8101 tcgtaaagaa ttcccgtata caccggtgca attctcacgc gtgccgccac catgggcgtt
     8161 aaagtccttt tcgccctgat ctgcattgcc gtcgcagagg ccgtttctaa gggtgaagaa
     8221 ttgttcaccg gagttgtgcc catcctggtt gagctggacg gagacgttaa cggacacaag
     8281 ttctcagtga gcggcgaagg agagggcgac gctacgtacg gtaaactgac gttgaagttc
     8341 atctgtacaa caggaaagct gccagtgccc tggcctacac tcgtgaccac cctcacctac
     8401 ggagtccagt gcttctcccg gtaccccgac cacatgaaac agcacgattt cttcaagtcc
     8461 gcgatgcctg agggctatgt tcaggaacgc accatattct tcaaggacga cggtaactat
     8521 aaaaccaggg ctgaagttaa gttcgagggg gacacgttgg taaatagaat tgagttgaaa
     8581 ggaatcgatt tcaaagaaga cggcaatatt ttgggccaca agctggagta caactacaac
     8641 agccacaacg tgtatattat ggctgataag cagaagaacg gcatcaaggt gaacttcaaa
     8701 atccgacaca atatcgagga tggctctgtg caactggccg accattacca gcaaaacacg
     8761 cctatcggcg acggtccggt tttgctgcca gataaccact acctctccac gcaatcagcc
     8821 ctctccaagg atcctaacga aaaaagagac catatggttc tgcttgaatt cgtgacggct
     8881 gctgggatca ccctgggaat ggatgagctg tataaggcca gcggcagcgg cgccacaaac
     8941 ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc ccgggcctca tatggtggat
     9001 ccaaaaaaga agagaaaggt agatccaaag aagaaaagga aggttggcag cggctccgct
     9061 cgggaccctc aacctttctt ccctcctctg tatctcggag gaccagagat cactactgag
     9121 aactgcgagc gggaacctat acatatacca ggttctattc aacctcatgg agcactgctc
     9181 acagccgatg gacattctgg cgaggtgctg caagtttcac tcaacgcagc cactttcctg
     9241 ggccaggaac caactgttct gcggggacag accctggcag ccctgctgcc agaccagtgg
     9301 cctgctctgc agactgccct gcctccaggc tgccaggacg ccctgcagta tcgcgccacc
     9361 ctggattggc ccgccgccgg acacctgagc ctgaccgtgc atagggtggc cgagctgctg
     9421 attctggaat tcgagcctac tgaggcatgg gactctattg gaccccacgc tctgagaaat
     9481 gcaatgttcg ctctggagag tgctcctaat ctccgggcac tggctgaagt cgcaacccaa
     9541 acagtccggg aactgtcagg tttcgaccgg gtgatgctgt acaagtttgc accagacgca
     9601 acaggagagg ttattgccga agcaaggcgc gagggcatgc aggcttacct cgggcatagg
     9661 tttcccgcat ccaccacccc tgcacaagct agggccctct acacaagaca cctgctccgg
     9721 ctgaccgcag acaccagggc tgcagcagtg cccctcgacc ccgtgctgaa tccccagaca
     9781 aatgctccta cacctctggg cggagctgtc ctcagagcta catccccaat gcacatgcag
     9841 tacctgagga atatgggagt gggctcctcc ctgagcgtca gcgtcgtggt cggcggccag
     9901 ctgtggggac tgattgtctg ccaccatcag acaccctacg tgctgccacc cgatctgcgg
     9961 accaccctgg agtatctggg gaggctcctg tccctgcagg tgcagaggaa agaagcctga
    10021 ttaattaagg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag
    10081 agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
    10141 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    10201 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    10261 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    10321 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    10381 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    10441 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    10501 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    10561 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    10621 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    10681 gagtcggatc tccctttggg ccgcctcccc gcat
//
