LOCUS       Exported                7493 bp ds-DNA     circular SYN 12-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 15, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..359
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
     misc_feature    360..1623
                     /locus_tag="beta-actin promoter"
                     /label=beta-actin promoter
     misc_feature    1626..1670
                     /locus_tag="Intron"
                     /label=Intron
     misc_feature    1772..1777
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    1784..3373
                     /locus_tag="cORF"
                     /label=cORF
     misc_feature    1784..2239
                     /locus_tag="eMagB"
                     /label=eMagB
     misc_feature    2294..3226
                     /locus_tag="iRFP"
                     /label=iRFP
     misc_feature    3234..3370
                     /locus_tag="OMP25"
                     /label=OMP25
     misc_feature    3374..3379
                     /locus_tag="HpaI"
                     /label=HpaI
     misc_feature    3413..3552
                     /locus_tag="Intron(1)"
                     /label=Intron
                     /label=Intron(1)
     misc_feature    3555..4156
                     /locus_tag="WPRE"
                     /label=WPRE
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcggtacc gcgggcccgg
     1741 gatccaccgg aagccacctg catgcggccg cgctagcgcc accatgggac ataccctcta
     1801 cgcgccgggg ggttatgaca tcatgggtta cctcagacag atcagaaacc ggccgaaccc
     1861 acaagtggag ctgggacccg tcgacctctc ctgcgccctc gtgctgtgtg accttaagca
     1921 gaaggacacc cctgtggtgt acgcctccga agcattcctg gagatgaccg ggtacaacag
     1981 acacgaagtg ctgggacgga actgccgctt cctgcaatcc ccggatggaa tggtgaagcc
     2041 taagtcaacc cgcaaatacg tggactccaa cactatctac accatgaaga aggccattga
     2101 ccgcaatgct gaggtgcaag tggaagtggt gaacttcaag aagaacggac agcgcttcgt
     2161 caacttcctg actatgattc ccgtgcggga cgaaaccggc gaataccggt acagcatcgg
     2221 gtttcagtgc gagactgagg gcggctccgg aggcagcggc ggttctggag gttccggtgg
     2281 cggctcccaa ttgatggcgc gtaaggtcga tctcacctcc tgcgatcgcg agccgatcca
     2341 catccccggc agcattcagc cgtgcggctg cctactagcc tgcgacgcgc aggcggtgcg
     2401 gatcacgcgc attacggaaa atgccggcgc gttctttgga cgcgaaactc cgcgggtcgg
     2461 tgagctactc gccgattact tcggcgagac cgaagcccat gcgctgcgca acgcactggc
     2521 gcagtcctcc gatccaaagc gaccggcgct gatcttcggt tggcgcgacg gcctgaccgg
     2581 ccgcaccttc gacatctcac tgcatcgcca tgacggtaca tcgatcatcg agttcgagcc
     2641 tgcggcggcc gaacaggccg acaatccgct gcggctgacg cggcagatca tcgcgcgcac
     2701 caaagaactg aagtcgctcg aagagatggc cgcacgggtg ccgcgctatc tgcaggcgat
     2761 gctcggctat caccgcgtga tgttgtaccg cttcgcggac gacggctccg ggatggtgat
     2821 cggcgaggcg aagcgcagcg acctagagag ctttctcggt cagcactttc cggcgtcgct
     2881 ggtcccgcag caggcgcggc tactgtactt gaagaacgcg atccgcgtgg tctcggattc
     2941 gcgcggcatc agcagccgga tcgtgcccga gcacgacgcc tccggcgccg cgctcgatct
     3001 gtcgttcgcg cacctgcgca gcatctcgcc ctgccatctc gaatttctgc ggaacatggg
     3061 cgtcagcgcc tcgatgtcgc tgtcgatcat cattgacggc acgctatggg gattgatcat
     3121 ctgtcatcat tacgagccgc gtgccgtgcc gatggcgcag cgcgtcgcgg ccgaaatgtt
     3181 cgccgacttc ttatcgctgc acttcaccgc cgcccaccac caacgctacg cgttgcaggt
     3241 gcagaatgga cctataggac atcgaggtga aggggaccca agtggtattc ccatatttat
     3301 ggtgctggtg ccagtgtttg ccctcaccat ggtagcagcc tgggctttca tgagataccg
     3361 gcaacaactt tgagttaact cgaggtaccg atgaatagct aaggtcgagg ccgcaggtaa
     3421 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
     3481 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
     3541 tctccacagg tgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3961 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4081 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4141 ccccgcctgg aattcgagct cggtacgatc agcctcgact gtgccttcta gttgccagcc
     4201 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4261 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4321 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggccagc
     4381 ttttgttccc tttagggtta attgcgcgct tggcgctaat catggtcata gctgtttcca
     4441 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     4501 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     4561 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     4621 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     4681 atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg
     4741 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     4801 agaaaagcct tgacttgagg ttagattttt ttttatattt tgtttgtgtt atttttttct
     4861 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     4921 ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcagcccaag
     4981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5161 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5341 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     5401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     5521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     5941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6181 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
