LOCUS       pHiFiCas9-2xsgRNA_(empty,_donor)_        9022 bp    DNA     circular UNA 23-SEP-2020
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...11-cfx6nfk
VERSION     urn.local...11-cfx6nfk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..249
     source          1..>433
     misc_feature    250..267
                     /note="Geneious type: Site"
                     /standard_name="gRNA cloning site"
     misc_feature    268..343
                     /note="chimeric guide RNA scaffold"
                     /standard_name="chimeric guide RNA scaffold"
     misc_feature    344..349
                     /note="U6 terminator"
                     /standard_name="U6 terminator"
     misc_feature    <434..863
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of peSpCas9(1.1) PITCh gRNA
                     extraction - Fragment 2 - source extraction 2
                     (concatenated sequence 2)"
     promoter        434..682
     source          434..>867
     misc_feature    683..702
                     /note="Geneious type: gRNA"
                     /standard_name="PITCh-gRNA#3"
     misc_feature    703..778
                     /note="chimeric guide RNA scaffold"
                     /standard_name="chimeric guide RNA scaffold"
     misc_feature    779..784
                     /note="U6 terminator"
                     /standard_name="U6 terminator"
     misc_feature    864..867
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    868..434
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of peSpCas9(1.1) (modified)
                     (concatenated sequence 1)"
     source          <868..9022
     misc_feature    869..1667
                     /note="CBh"
                     /note="Geneious type: Promoter"
                     /standard_name="CBh"
     misc_feature    1619..1644
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     misc_feature    1669..1679
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     misc_feature    1674..1679
                     /note="Geneious type: Kozak"
                     /standard_name="Kozak"
     misc_feature    1680..6038
                     /note="Geneious type: ORF"
                     /standard_name="ORF 1 (frame 3)"
     source          <1680..>6038
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1683..6034
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GACTATAAGGACCACGACGGAGACTACAAGGATCATGATATT
                     GATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCC
                     ACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTC
                     TGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTG
                     CTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCG
                     ACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACAC
                     CAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAG
                     GTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGA
                     AGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAA
                     GTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGAC
                     CTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGA
                     TCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGT
                     GCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCC
                     AAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCC
                     AGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGG
                     CCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTG
                     AGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGT
                     ACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACAT
                     CCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGA
                     TACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGC
                     CTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACAT
                     TGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAG
                     ATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGC
                     AGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGC
                     CATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATC
                     GAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACA
                     GCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGA
                     GGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTC
                     GATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACT
                     TCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCC
                     CGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAAC
                     CGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCG
                     ACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCA
                     CGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGAC
                     ATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGG
                     AACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCG
                     GCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGAC
                     AAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAA
                     ACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGC
                     CCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGC
                     CCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAG
                     TGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGAC
                     CACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATC
                     AAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGA
                     ACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGA
                     ACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTT
                     CTGGCGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCA
                     AGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCA
                     GCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAG
                     AGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAA
                     CCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTA
                     CGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTG
                     GTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACC
                     ACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTA
                     CCCTGCGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAG
                     ATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACA
                     GCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAA
                     GGCGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGG
                     GATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGA
                     CCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGA
                     TAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGC
                     CCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGA
                     AACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGA
                     GAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTG
                     ATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGC
                     TGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGT
                     GAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAAT
                     GAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGC
                     AGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCT
                     GTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATC
                     CACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCA
                     CCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCA
                     CCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGAC
                     AAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAA"
     misc_feature    1683..1736
                     /note="Geneious type: Sorting signal"
                     /standard_name="BPNLS"
     CDS             1716..1736
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /translation="PKKKRKV"
                     /standard_name="nuclear localization signal of SV40 large
                     T  antigen CDS"
     CDS             1776..5879
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /standard_name="Cas9 (Csn1) endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system CDS"
     misc_feature    complement(3827..3862)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="HiFiCas9 R691A mutation RV"
     primer_bind     3835..3870
                     /standard_name="HiFiCas9 R691A mutation FW"
     misc_feature    3846..3848
                     /note="Geneious type: mutation"
                     /standard_name="R691A"
     misc_feature    5892..5945
                     /note="Geneious type: Sorting signal"
                     /standard_name="BPNLS"
     CDS             5925..5945
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /translation="PKKKRKV"
                     /standard_name="nuclear localization signal of SV40 large
                     T  antigen CDS"
     CDS             5952..6017
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /standard_name="three tandem FLAG(R) epitope tags,
                     followed by an enterokinase cleavage site CDS"
     misc_feature    6045..6276
                     /note="bGH polyA"
                     /note="Geneious type: polyA_signal"
                     /standard_name="bGH polyA"
     misc_feature    complement(6102..6117)
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     misc_feature    6285..6425
                     /standard_name="R-ITR"
     rep_origin      6517..6823
     misc_feature    7342..8199
                     /note="Geneious type: Marker"
                     /standard_name="AmpR"
     rep_origin      8350..9017
     primer_bind     9003..1
                     /standard_name="Sequsence primer"
ORIGIN      
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      421 aaatggctct agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
      481 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
      541 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      601 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      661 cttgtggaaa ggacgaaaca ccgcatcgta cgcgtacgtg ttgttttaga gctagaaata
      721 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      781 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc
      841 gccaattctg cagacaaatg gcggtacccg ttacataact tacggtaaat ggcccgcctg
      901 gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc
      961 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     1021 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     1081 gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     1141 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     1201 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     1261 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     1321 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     1381 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1441 ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     1501 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     1561 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
     1621 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
     1681 tgaagcggac tgctgatggc agtgaatttg agtccccaaa gaagaagaga aaggtggaag
     1741 gtatccacgg agtcccagca gccgaattcg ccaccatgga caagaagtat tctatcggac
     1801 tggacatcgg gactaatagc gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct
     1861 ctaagaagtt caaggtgctc gggaacaccg accggcattc catcaagaaa aatctgatcg
     1921 gagctctcct ctttgattca ggggagaccg ctgaagcaac ccgcctcaag cggactgcta
     1981 gacggcggta caccaggagg aagaaccgga tttgttacct tcaagagata ttctccaacg
     2041 aaatggcaaa ggtcgacgac agcttcttcc ataggctgga agaatcattc ctcgtggaag
     2101 aggataagaa gcatgaacgg catcccatct tcggtaatat cgtcgacgag gtggcctatc
     2161 acgagaaata cccaaccatc taccatcttc gcaaaaagct ggtggactca accgacaagg
     2221 cagacctccg gcttatctac ctggccctgg cccacatgat caagttcaga ggccacttcc
     2281 tgatcgaggg cgacctcaat cctgacaata gcgatgtgga taaactgttc atccagctgg
     2341 tgcagactta caaccagctc tttgaagaga accccatcaa tgcaagcgga gtcgatgcca
     2401 aggccattct gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt atcgctcagc
     2461 tgccgggtga aaagaaaaat ggactgttcg ggaacctgat tgctctttca cttgggctga
     2521 ctcccaattt caagtctaat ttcgacctgg cagaggatgc caagctgcaa ctgtccaagg
     2581 acacctatga tgacgatctc gacaacctcc tggcccagat cggtgaccaa tacgccgacc
     2641 ttttccttgc tgctaagaat ctttctgacg ccatcctgct gtctgacatt ctccgcgtga
     2701 acactgaaat caccaaggcc cctctttcag cttcaatgat taagcggtat gatgagcacc
     2761 accaggacct gaccctgctt aaggcactcg tccggcagca gcttccggag aagtacaagg
     2821 aaatcttctt tgaccagtca aagaatggat acgccggcta catcgacgga ggtgcctccc
     2881 aagaggaatt ttataagttt atcaaaccta tccttgagaa gatggacggc accgaagagc
     2941 tcctcgtgaa actgaatcgg gaggatctgc tgcggaagca gcgcactttc gacaatggga
     3001 gcattcccca ccagatccat cttggggagc ttcacgccat ccttcggcgc caagaggact
     3061 tctacccctt tcttaaggac aacagggaga agattgagaa aattctcact ttccgcatcc
     3121 cctactacgt gggacccctc gccagaggaa atagccggtt tgcttggatg accagaaagt
     3181 cagaagaaac tatcactccc tggaacttcg aagaggtggt ggacaaggga gccagcgctc
     3241 agtcattcat cgaacggatg actaacttcg ataagaacct ccccaatgag aaggtcctgc
     3301 cgaaacattc cctgctctac gagtacttta ccgtgtacaa cgagctgacc aaggtgaaat
     3361 atgtcaccga agggatgagg aagcccgcat tcctgtcagg cgaacaaaag aaggcaattg
     3421 tggaccttct gttcaagacc aatagaaagg tgaccgtgaa gcagctgaag gaggactatt
     3481 tcaagaaaat tgaatgcttc gactctgtgg agattagcgg ggtcgaagat cggttcaacg
     3541 caagcctggg tacctaccat gatctgctta agatcatcaa ggacaaggat tttctggaca
     3601 atgaggagaa cgaggacatc cttgaggaca ttgtcctgac tctcactctg ttcgaggacc
     3661 gggaaatgat cgaggagagg cttaagacct acgcccatct gttcgacgat aaagtgatga
     3721 agcaacttaa acggagaaga tataccggat ggggacgcct tagccgcaaa ctcatcaacg
     3781 gaatccggga caaacagagc ggaaagacca ttcttgattt ccttaagagc gacggattcg
     3841 ctaatgccaa cttcatgcaa cttatccatg atgattccct gacctttaag gaggacatcc
     3901 agaaggccca agtgtctgga caaggtgact cactgcacga gcatatcgca aatctggctg
     3961 gttcacccgc tattaagaag ggtattctcc agaccgtgaa agtcgtggac gagctggtca
     4021 aggtgatggg tcgccataaa ccagagaaca ttgtcatcga gatggccagg gaaaaccaga
     4081 ctacccagaa gggacagaag aacagcaggg agcggatgaa aagaattgag gaagggatta
     4141 aggagctcgg gtcacagatc cttaaagagc acccggtgga aaacacccag cttcagaatg
     4201 agaagctcta tctgtactac cttcaaaatg gacgcgatat gtatgtggac caagagcttg
     4261 atatcaacag gctctcagac tacgacgtgg accacatcgt ccctcagagc ttcctcaaag
     4321 acgactcaat tgacaataag gtgctgactc gctcagacaa gaaccgggga aagtcagata
     4381 acgtgccctc agaggaagtc gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg
     4441 caaagctgat cactcagcgg aagttcgaca atctcactaa ggctgagagg ggcggactga
     4501 gcgaactgga caaagcagga ttcattaaac ggcaacttgt ggagactcgg cagattacta
     4561 aacatgtcgc ccaaatcctt gactcacgca tgaataccaa gtacgacgaa aacgacaaac
     4621 ttatccgcga ggtgaaggtg attaccctga agtccaagct ggtcagcgat ttcagaaagg
     4681 actttcaatt ctacaaagtg cgggagatca ataactatca tcatgctcat gacgcatatc
     4741 tgaatgccgt ggtgggaacc gccctgatca agaagtaccc aaagctggaa agcgagttcg
     4801 tgtacggaga ctacaaggtc tacgacgtgc gcaagatgat tgccaaatct gagcaggaga
     4861 tcggaaaggc caccgcaaag tacttcttct acagcaacat catgaatttc ttcaagaccg
     4921 aaatcaccct tgcaaacggt gagatccgga agaggccgct catcgagact aatggggaga
     4981 ctggcgaaat cgtgtgggac aagggcagag atttcgctac cgtgcgcaaa gtgctttcta
     5041 tgcctcaagt gaacatcgtg aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat
     5101 caatcctccc caagcgcaac tccgacaagc tcattgcaag gaagaaggat tgggacccta
     5161 agaagtacgg cggattcgat tcaccaactg tggcttattc tgtcctggtc gtggctaagg
     5221 tggaaaaagg aaagtctaag aagctcaaga gcgtgaagga actgctgggt atcaccatta
     5281 tggagcgcag ctccttcgag aagaacccaa ttgactttct cgaagccaaa ggttacaagg
     5341 aagtcaagaa ggaccttatc atcaagctcc caaagtatag cctgttcgaa ctggagaatg
     5401 ggcggaagcg gatgctcgcc tccgctggcg aacttcagaa gggtaatgag ctggctctcc
     5461 cctccaagta cgtgaatttc ctctaccttg caagccatta cgagaagctg aaggggagcc
     5521 ccgaggacaa cgagcaaaag caactgtttg tggagcagca taagcattat ctggacgaga
     5581 tcattgagca gatttccgag ttttctaaac gcgtcattct cgctgatgcc aacctcgata
     5641 aagtccttag cgcatacaat aagcacagag acaaaccaat tcgggagcag gctgagaata
     5701 tcatccacct gttcaccctc accaatcttg gtgcccctgc cgcattcaag tacttcgaca
     5761 ccaccatcga ccggaaacgc tatacctcca ccaaagaagt gctggacgcc accctcatcc
     5821 accagagcat caccggactt tacgaaactc ggattgacct ctcacagctc ggaggggatg
     5881 agggagctga taagcgcacc gccgatggtt ccgagttcga aagccccaaa aaaaagcgca
     5941 aggtcgaaga tgactataag gatcacgacg gtgattataa agaccacgat atcgactata
     6001 aagacgacga tgacaaagag ctgtataaac tgctgtaaga attcctagag ctcgctgatc
     6061 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6121 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6181 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6241 ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat
     6301 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     6361 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     6421 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6481 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     6721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6841 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     6961 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7021 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7081 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7141 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7201 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7261 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7321 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7381 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7441 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7501 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7561 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7621 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7681 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7741 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7801 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7861 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7921 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7981 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8041 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc
     8101 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8161 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9001 tgctggcctt ttgctcacat gt
//
