LOCUS       pEPQD0KN0025_(pJL1-ATG_ 2355 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1637..1656
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(526..542)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(830..1645)
                     /label="aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1553..1572
                     /label="Kan-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(526..542)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     212..231
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(207..530)
                     /label="lacZ-alpha"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(539..561)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    772..786
                     /label="T7 term hairpin F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    28..104
                     /label="T7 promoter and 5' UTR from pJL1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(138..674)
                     /label="lacZ expression casette"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(793..807)
                     /label="T7 term hairpin R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(537..660)
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(751..769)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      765..812
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    457..513
                     /label="MCS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    619..640
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    550..566
                     /label="lac operator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        28..46
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(81..110)
                     /label="AN163\vector\amp\rev"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             78..100
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      1767..2355
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(574..604)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    706..812
                     /label="3' UTR and T7 terminator from pJL1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2256..2275
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     439..456
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    93..99
                     /label="RBS"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     CDS             complement(207..530)
                     /label="lacZ beta-galactosidase alpha peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     425..447
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    join(746..2355,1..24)
                     /label="backbone from pJL1-sfGFP (Addgene #69496)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     440..456
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2292..2310
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(877..904)
                     /label="QMD063_pJL1_outside(kanR)_R"
                     /note="sequence: tttcaaaaatatggtattgataatcctg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 aacgccagca acgcgatccc gcgaaattaa tacgactcac tatagggaca ccacaacggt
       61 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacaAATG tGAGACCgtc
      121 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      181 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      241 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      301 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      361 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      421 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga
      481 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
      541 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      601 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      661 cgctttccag tcgggaaacc tgtcgtgcca gctaGGTCTC aGCTTcggct gctaacaaag
      721 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      781 gggcctctaa acgggtcttg aggggttttt tgctgaaagc caattctgat tagaaaaact
      841 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      901 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      961 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1021 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1081 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1141 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1201 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1261 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     1321 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     1381 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1441 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1501 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1561 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ttcgagcaag
     1621 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1681 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1741 acacaacgtg agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2341 cggagcctat ggaaa
//