LOCUS       pEPQD0KN0245_(pJL1-ATG_ 2354 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(410..426)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1437..1456
                     /label="Kan-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     309..331
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(458..488)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(423..445)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     323..340
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2267..2285
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(714..1529)
                     /label="aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      649..696
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     96..115
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(91..414)
                     /label="lacZ beta-galactosidase alpha peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             2317..2339
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    503..524
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2267..2343
                     /label="T7 promoter and 5' UTR from pJL1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     324..340
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    434..450
                     /label="lac operator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(421..544)
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(677..691)
                     /label="T7 term hairpin R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(635..653)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(22..558)
                     /label="lacZ expression casette"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     2140..2159
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(406..426)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    630..2263
                     /label="backbone from pJL1-sfGFP (Addgene #69496)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2332..2338
                     /label="RBS"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     rep_origin      1651..2239
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1521..1540
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    341..397
                     /label="MCS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    590..696
                     /label="3' UTR and T7 terminator from pJL1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    656..670
                     /label="T7 term hairpin F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2176..2194
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(761..788)
                     /label="QMD063_pJL1_outside(kanR)_R"
                     /note="sequence: tttcaaaaatatggtattgataatcctg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          join(2344..2354,1..14)
                     /label="QMD380_25pJL1accept_to_CCAT_F"
                     /note="sequence: tctGAAGACtcACCATtGAGACCgtcacagcttgtc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2307..2343)
                     /label="QMD381_25pJL1accept_to_CCAT_R"
                     /note="sequence: tctGAAGACtcTGGTtatatctccttcttaaagttaaacaaaattatttcta"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 Cgtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
       61 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      121 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
      181 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      241 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      301 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
      361 gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
      421 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      481 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      541 tgcccgcttt ccagtcggga aacctgtcgt gccagctaGG TCTCaGCTTc ggctgctaac
      601 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      661 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagccaattc tgattagaaa
      721 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
      781 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
      841 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
      901 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
      961 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     1021 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1081 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     1141 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     1201 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     1261 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     1321 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     1381 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     1441 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcttcgag
     1501 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     1561 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     1621 tgagacacaa cgtgagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2221 ggggcggagc ctatggaaaa acgccagcaa cgcgatcccg cgaaattaat acgactcact
     2281 atagggacac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     2341 ataACCATtG AGAC
//