LOCUS       pEPQD0CB0026_(pEU-ACCEP 4281 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    795..811
                     /label="lac operator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    241..258
                     /label="SP6 Promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    259..330
                     /label="E01 Translational Enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2769..3629)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    985..1315
                     /label="E coli genome fragment"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1062..1313)
                     /label="diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1  from E coli MG1655"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(4030..4281)
                     /label="tet"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1621..1643)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(2010..2598)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(1657..1686)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(767..787)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(3671..3699)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(784..806)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     684..701
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(782..905)
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    864..885
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(452..775)
                     /label="lacZ beta-galactosidase alpha peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1009..1022
                     /label="Hairpin F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        complement(819..849)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    985..1061
                     /label="folA/apaH terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(383..919)
                     /label="lacZ expression casette"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1028..1042)
                     /label="Hairpin R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     457..476
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3858..3880)
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(357..362)
                     /label="Bsai"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     670..692
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    702..758
                     /label="MCS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1916..1938
                     /label="QMD542_211or_binding_seq_R"
                     /note="sequence: TATCCACAGAATCAGGGGATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          3734..3753
                     /label="QMD312_L0_alternate to 212or_R"
                     /note="sequence: GCACATTTCCCCGAAAAGTG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2086..2109)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3674..3701
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1608..1624)
                     /label="QMD512_M13_R"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          951..973
                     /label="QMD046 binding region (61.3)"
                     /note="sequence: CGGGTTATAATTACCTCAGGTCG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2057..2076)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1621..1643)
                     /label="QMD066_pEU_outsideterm(M13pUC)_R"
                     /note="sequence: AGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          356..375
                     /label="QMD044 binding region (60.6)"
                     /note="sequence: tgagaccgtcacagcttgtc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          980..1004
                     /label="CFPSQMD002"
                     /note="sequence: CATGGTTTTGTATAGAATTTACGGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(784..806)
                     /label="QMD066_pEU_outsideterm(M13pUC)_R"
                     /note="sequence: AGCGGATAACAATTTCACACAGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1914..1939)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2055..2073)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2896..2912)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1052..1070)
                     /label="QMD065_pEU_outside_R"
                     /note="sequence: GCGTCTTAAACACCGCCTG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          685..701
                     /label="QMD231_M13_forward20_primer"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3893..3912)
                     /label="QMD129_MEKKIBsaI_lift_R, 61.3"
                     /note="sequence: ggcatccgcttacagacaag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2555..2576
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(771..787)
                     /label="QMD512_M13_R"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(370..389)
                     /label="QMD129_MEKKIBsaI_lift_R, 61.3"
                     /note="sequence: ggcatccgcttacagacaag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          151..174
                     /label="QMD064_pEU_outside_F"
                     /note="sequence: TAAGCCAGATGCTACACAATTAGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          670..692
                     /label="QMD229_M13_pUC_bindslacZ_F"
                     /note="sequence: cccagtcacgacgttgtaaaacg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(328..355)
                     /label="QMD049 binding region (60.6)"
                     /note="sequence: cattAGCTCGAGAACTAGTGATATCTTG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 GGCGTAGAGG ATCTGGCTAG CGATGACCCT GCTGATTGGT TCGCTGACCA TTTCCGGGTG
       61 CGGGACGGCG TTACCAGAAA CTCAGAAGGT TCGTCCAACC AAACCGACTC TGGCGGCAGT
      121 TTACGAGAGA GATGATAGGG TCTGCTTCAG TAAGCCAGAT GCTACACAAT TAGGCTTGTA
      181 CATACTGTCG TTAGAACGCG GCTACAATTA ATACATAACC TTATGTATCA TACACATACG
      241 ATTTAGGTGA CACTATAGAA CTCACCTATC TCCCCAACAC CTAATAACAT TCAATCACTC
      301 TTTCCACTAA CCACCTATCT ACATCACCAA GATATCACTA GTTCTCGAGC TaatgtGAGA
      361 CCgtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      421 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
      481 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
      541 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      601 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      661 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc
      721 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
      781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      901 ctgcccgctt tccagtcggG aaacctgtcg tgccagctaG GTCTCaGCTT CGGGTTATAA
      961 TTACCTCAGG TCGACGTCCC ATGGTTTTGT ATAGAATTTA CGGCTAGCGC CGGATGCGAC
     1021 GCCGGTCGCG TCTTATCCGG CCTTCCTATA TCAGGCGGTG TTTAAGACGC CGCCGCTTCG
     1081 CCCAAATCCT TATGCCGGTT CGACGACTGG ACAAAATACT GTTTATCTTC CCAGCGCAGG
     1141 CAGGTTAATG TACCACCCCA GCAGCAGCCG GTATCCAGCG CGTATATACC TTCCGGCGTA
     1201 CCTTTGCCCT CCAGCGATGC CCAGTGACCA AAGGCGATGC TGTATTCTTC AGCGACAGGG
     1261 CCAGGAATCG CAAACCACGG TTTCAGTGGG GCAGGGGCCT CTTCCGGCGA TTCTTACTAG
     1321 CTAGTATGCA TAGGTGCTGA AATATAAAGT TTGTGTTTCT AAAACACACG TGGTACGTAC
     1381 GATAACGTAC AGTGTTTTTC CCTCCACTTA AATCGAAGGG TAGTGTCTTG GAGCGCGCGG
     1441 AGTAAACATA TATGGTTCAT ATATGTCCGT AGGCACGTAA AAAAAGCGAG GGATTCGAAT
     1501 TCCCCCGGAA CCCCCGGTTG GGGCCCACGC CTCGATCGAG CAAAAAAAAA AAAAAAGAAA
     1561 AAAAAAAAAA AAAAAAGCTT TCCCGCGGCC AGCTTGGCGT AATCATGGTC ATAGCTGTTT
     1621 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
     1681 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
     1741 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
     1801 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CACTCACTGA CTCGCTGCGC
     1861 TCGGTCGCTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     1921 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     1981 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     2041 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     2101 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     2161 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     2221 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     2281 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     2341 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     2401 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     2461 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     2521 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     2581 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2641 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2701 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2761 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2821 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2881 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAtCCACGCT CACCGGCTCC AGATTTATCA
     2941 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3001 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3061 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3121 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3181 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3241 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3301 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGCG TATGCGGCGA
     3361 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3421 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3481 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3541 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3601 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3661 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3721 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT
     3781 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC
     3841 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     3901 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     3961 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATTCGACG
     4021 CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG
     4081 CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC
     4141 GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG
     4201 ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT
     4261 GATGCCGGCC ACGATGCGTC C
//