LOCUS       pEPQD0KN0284_(pJL1_WG-p 3126 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(join(2929..3126,1..126))
                     /label="lacZ beta-galactosidase alpha peptide"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(170..200)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     21..43
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    336..412
                     /label="folA/apaH terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(join(2860..3126,1..270))
                     /label="lacZ expression casette"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    360..373
                     /label="Hairpin F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    336..666
                     /label="E coli genome fragment"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(995..1013)
                     /label="T7 Term"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     35..52
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(951..971)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(379..393)
                     /label="Hairpin R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     1881..1900
                     /label="pENTR-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    215..236
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    53..109
                     /label="MCS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    990..2623
                     /label="backbone from pJL1-sfGFP (Addgene #69496)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      2011..2599
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1016..1030
                     /label="T7 term hairpin F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      1009..1056
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    146..162
                     /label="lac operator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(413..664)
                     /label="diadenosine tetraphosphatase (apaH) C-terminal fragment ( aa 198-280) AVI54590.1  from E coli MG1655"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(1074..1889)
                     /label="aminoglycoside O-phosphotransferase APH(3')-Ia, kanamycin resistance (kanR)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(1037..1051)
                     /label="T7 term hairpin R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(133..256)
                     /label="Plac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2718..2735
                     /label="SP6 Promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(118..138)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2736..2807
                     /label="E01 Translational Enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(1121..1148)
                     /label="QMD063_pJL1_outside(kanR)_R"
                     /note="sequence: tttcaaaaatatggtattgataatcctg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2536..2554
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(403..421)
                     /label="QMD065_pEU_outside_R"
                     /note="sequence: GCGTCTTAAACACCGCCTG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
       61 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat
      121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      241 cgttgcgctc actgcccgct ttccagtcgg Gaaacctgtc gtgccagcta GGTCTCaGCT
      301 TCGGGTTATA ATTACCTCAG GTCGACGTCC CATGGTTTTG TATAGAATTT ACGGCTAGCG
      361 CCGGATGCGA CGCCGGTCGC GTCTTATCCG GCCTTCCTAT ATCAGGCGGT GTTTAAGACG
      421 CCGCCGCTTC GCCCAAATCC TTATGCCGGT TCGACGACTG GACAAAATAC TGTTTATCTT
      481 CCCAGCGCAG GCAGGTTAAT GTACCACCCC AGCAGCAGCC GGTATCCAGC GCGTATATAC
      541 CTTCCGGCGT ACCTTTGCCC TCCAGCGATG CCCAGTGACC AAAGGCGATG CTGTATTCTT
      601 CAGCGACAGG GCCAGGAATC GCAAACCACG GTTTCAGTGG GGCAGGGGCC TCTTCCGGCG
      661 ATTCTTACTA GCTAGTATGC ATAGGTGCTG AAATATAAAG TTTGTGTTTC TAAAACACAC
      721 GTGGTACGTA CGATAACGTA CAGTGTTTTT CCCTCCACTT AAATCGAAGG GTAGTGTCTT
      781 GGAGCGCGCG GAGTAAACAT ATATGGTTCA TATATGTCCG TAGGCACGTA AAAAAAGCGA
      841 GGGATTCGAA TTCCCCCGGA ACCCCCGGTT GGGGCCCACG CCTCGATCGA GCAAAAAAAA
      901 AAAAAGAAAA AAAAAAAAAA AAAGCTTTCC CGCGGCCAGC TTGGCGTAAT CATGGTCATA
      961 GCTGTTTCCT GTGTGAAATT GTTATCCGCt gccaccgctg agcaataact agcataaccc
     1021 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagccaattc tgattagaaa
     1081 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     1141 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     1201 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     1261 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     1321 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     1381 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1441 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     1501 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     1561 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     1621 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     1681 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     1741 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     1801 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcttcgag
     1861 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     1921 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     1981 tgagacacaa cgtgagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcgatcccg cgaCGAATAA GCCAGATGCT
     2641 ACACAATTAG GCTTGTACAT ACTGTCGTTA GAACGCGGCT ACAATTAATA CATAACCTTA
     2701 TGTATCATAC ACATACGATT TAGGTGACAC TATAGAACTC ACCTATCTCC CCAACACCTA
     2761 ATAACATTCA ATCACTCTTT CCACTAACCA CCTATCTACA TCACCAAGAT ATCACTAGTT
     2821 CTCGAGCTAA TGaGAGAccg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2881 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2941 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     3001 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaag
//