LOCUS       pEPQD0CM0541_(GST-throm 2712 bp ds-DNA     circular     08-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(1018..1036)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    40..84
                     /label="manually adjusted for minimal hairpin, -1.35 kcal/mol with pJL1-aCCAT 5'UTR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    join(729..2712,1..29)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    43..716
                     /label="N-term tag from pEPQD0CM0541"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1053..1072)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     651..673
                     /label="pGEX 5'"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             700..716
                     /label="thrombin cleavage site LVPR/GS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(973..1561)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    43..693
                     /label="GST (glutathione S-transferase)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            complement(1809..2468)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2672..2689
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(781..801)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GAATAAGGAA GTGCCATTCC GCCTGACCTA GGTCTCTCCA TGTCACCGAT TTTAGGCTAC
       61 TGGAAAATTA AGGGTCTTGT GCAACCCACA CGTCTGCTGC TGGAGTACTT AGAGGAGAAG
      121 TACGAGGAGC ACCTCTACGA ACGCGACGAG GGCGACAAGT GGCGTAATAA GAAATTCGAG
      181 TTAGGCTTAG AATTCCCTAA CCTGCCGTAC TACATCGACG GCGACGTCAA GCTGACCCAA
      241 AGTATGGCGA TTATCCGCTA CATTGCCGAT AAACATAATA TGCTCGGCGG CTGCCCGAAG
      301 GAACGCGCTG AAATCTCCAT GTTAGAGGGC GCTGTCCTGG ACATCCGTTA TGGGGTGTCA
      361 CGTATCGCGT ACTCGAAGGA TTTCGAGACT CTGAAGGTGG ACTTCCTGTC GAAATTACCC
      421 GAGATGTTAA AGATGTTTGA GGACCGCTTG TGCCACAAGA CTTACCTGAA CGGCGACCAC
      481 GTGACTCACC CGGATTTTAT GCTGTACGAT GCCTTGGACG TCGTACTGTA TATGGATCCG
      541 ATGTGTTTGG ACGCCTTTCC GAAGCTGGTG TGCTTCAAGA AGCGCATCGA GGCCATTCCG
      601 CAGATCGACA AATATTTAAA GTCGTCAAAA TACATTGCGT GGCCGCTTCA GGGCTGGCAA
      661 GCCACGTTTG GTGGCGGGGA TCACCCACCT AAGAGCGACC TGGTTCCGCG TGGATCAATG
      721 AGAGACCTAG GCTAGGTGGA GGCTCAGTGA TGATAAGTCT GCGATGGTGG ATGCATGTGT
      781 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CAGAGGGCAC AATCCTATTC
      841 CGCGCTATCC GACAATCTCC AAGACATTAG GTGGAGTTCA GTTCGGCGTA TGGCATATGT
      901 CGCTGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
      961 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1021 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1081 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1141 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1201 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1261 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1321 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1381 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1441 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1501 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1561 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCTA TTCAACAAAG CCGCCGTCCC
     1621 GTCAAGTCAG CGTAAATGGG TAGGGGGCTT CAAATCGTCC CCCCATACGA TATAAGTTGT
     1681 TACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA
     1741 AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG
     1801 ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
     1861 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
     1921 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
     1981 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
     2041 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
     2101 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
     2161 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
     2221 ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA
     2281 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG
     2341 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC
     2401 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT
     2461 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     2521 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT
     2581 TGATACCTGA AACAAAACCC ATCGTACGGC CAAGGAAGTC TCCAATAACT GTGATCCACC
     2641 ACAAGCGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC ATGCATAATC
     2701 CGCACGCATC TG
//