LOCUS       pEPQD0CM0543_(GST-TEV_C 2712 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(1024..1043)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    14..687
                     /label="N-term tag from pEPQD0CM0543"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     2643..2660
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(1780..2439)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(989..1007)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    668..687
                     /label="TEV cleavage site ENLYFQ/S"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    700..2712
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    14..664
                     /label="GST (glutathione S-transferase)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(944..1532)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(752..772)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 AGGTCTCTCC ATGTCACCGA TTTTAGGCTA CTGGAAAATT AAGGGTCTTG TGCAACCCAC
       61 ACGTCTGCTG CTGGAGTACT TAGAGGAGAA GTACGAGGAG CACCTCTACG AACGCGACGA
      121 GGGCGACAAG TGGCGTAATA AGAAATTCGA GTTAGGCTTA GAATTCCCTA ACCTGCCGTA
      181 CTACATCGAC GGCGACGTCA AGCTGACCCA AAGTATGGCG ATTATCCGCT ACATTGCCGA
      241 TAAACATAAT ATGCTCGGCG GCTGCCCGAA GGAACGCGCT GAAATCTCCA TGTTAGAGGG
      301 CGCTGTCCTG GACATCCGTT ATGGGGTGTC ACGTATCGCG TACTCGAAGG ATTTCGAGAC
      361 TCTGAAGGTG GACTTCCTGT CGAAATTACC CGAGATGTTA AAGATGTTTG AGGACCGCTT
      421 GTGCCACAAG ACTTACCTGA ACGGCGACCA CGTGACTCAC CCGGATTTTA TGCTGTACGA
      481 TGCCTTGGAC GTCGTACTGT ATATGGATCC GATGTGTTTG GACGCCTTTC CGAAGCTGGT
      541 GTGCTTCAAG AAGCGCATCG AGGCCATTCC GCAGATCGAC AAATATTTAA AGTCGTCAAA
      601 ATACATTGCG TGGCCGCTTC AGGGCTGGCA AGCCACGTTT GGTGGCGGGG ATCACCCACC
      661 TAAGAGCGAG AACCTGTATT TTCAGTCAAT GAGAGACCTA GGCTAGGTGG AGGCTCAGTG
      721 ATGATAAGTC TGCGATGGTG GATGCATGTG TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
      781 TGTTATCCGC TCAGAGGGCA CAATCCTATT CCGCGCTATC CGACAATCTC CAAGACATTA
      841 GGTGGAGTTC AGTTCGGCGT ATGGCATATG TCGCTGGAAA GAACATGTGA GCAAAAGGCC
      901 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
      961 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
     1021 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
     1081 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
     1141 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
     1201 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
     1261 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
     1321 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
     1381 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
     1441 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
     1501 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
     1561 CTGACGCTCT ATTCAACAAA GCCGCCGTCC CGTCAAGTCA GCGTAAATGG GTAGGGGGCT
     1621 TCAAATCGTC CCCCCATACG ATATAAGTTG TTACTAGTGC TTGGATTCTC ACCAATAAAA
     1681 AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT
     1741 GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC
     1801 ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC
     1861 ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC
     1921 CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT
     1981 GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA
     2041 ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG
     2101 GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC
     2161 GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG
     2221 AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
     2281 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
     2341 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
     2401 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
     2461 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
     2521 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTGATACCTG AAACAAAACC CATCGTACGG
     2581 CCAAGGAAGT CTCCAATAAC TGTGATCCAC CACAAGCGCC AGGGTTTTCC CAGTCACGAC
     2641 GTTGTAAAAC GACGGCCAGT CATGCATAAT CCGCACGCAT CTGGAATAAG GAAGTGCCAT
     2701 TCCGCCTGAC CT
//