LOCUS       pEPQD0CM0544_(MBP-polya 3198 bp ds-DNA     circular     26-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(38..697)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(2480..2499)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2130..2141
                     /label="Factor Xa cleavage site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2082..2140
                     /label="spacer and factor Xa site"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             984..2078
                     /label="MBP (maltose binding protein), V313"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    984..2141
                     /label="N-term tag from pEPQD0CM0544"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(2445..2463)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(2400..2988)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     901..918
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    join(2156..3198,1..970)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2208..2228)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ATCTATCAAC AGGAGTCCAA GCGAGCTCGA TATCAAATTA CGCCCCGCCC TGCCACTCAT
       61 CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT
      121 GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA
      181 TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA
      241 AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT
      301 AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA
      361 AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG
      421 TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGAA
      481 ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT
      541 GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT
      601 AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA
      661 TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA
      721 ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG
      781 AACCTCTTAC GTGCCGATCA ACGTCTCATT GATACCTGAA ACAAAACCCA TCGTACGGCC
      841 AAGGAAGTCT CCAATAACTG TGATCCACCA CAAGCGCCAG GGTTTTCCCA GTCACGACGT
      901 TGTAAAACGA CGGCCAGTCA TGCATAATCC GCACGCATCT GGAATAAGGA AGTGCCATTC
      961 CGCCTGACCT AGGTCTCTCC ATGAAAATCG AAGAAGGCAA ATTAGTGATA TGGATCAATG
     1021 GAGATAAGGG ATACAATGGC TTAGCGGAAG TGGGAAAGAA GTTTGAAAAG GACACTGGGA
     1081 TCAAGGTTAC GGTCGAACAC CCAGACAAGT TAGAGGAAAA GTTTCCGCAA GTAGCAGCCA
     1141 CAGGGGACGG TCCGGATATC ATTTTCTGGG CCCATGATCG TTTCGGCGGA TATGCCCAGA
     1201 GTGGTCTTTT AGCGGAGATT ACTCCTGATA AGGCATTTCA AGATAAATTG TACCCTTTCA
     1261 CGTGGGACGC GGTTCGCTAT AATGGAAAAC TTATCGCATA TCCTATTGCG GTAGAGGCAC
     1321 TGAGTCTCAT CTACAATAAG GACTTGCTCC CTAATCCGCC TAAGACATGG GAAGAAATTC
     1381 CCGCCCTCGA CAAGGAGCTC AAGGCAAAGG GCAAATCCGC CTTGATGTTT AATCTCCAGG
     1441 AGCCATATTT TACTTGGCCG TTGATCGCGG CCGATGGCGG CTACGCCTTT AAATACGAGA
     1501 ATGGTAAATA TGATATCAAG GATGTCGGAG TAGACAATGC GGGTGCAAAG GCTGGACTTA
     1561 CTTTCTTAGT GGATTTGATC AAGAATAAGC ATATGAACGC TGATACGGAC TATAGTATTG
     1621 CCGAGGCGGC GTTCAACAAG GGTGAGACTG CCATGACTAT TAATGGACCT TGGGCTTGGT
     1681 CGAATATTGA TACGAGTAAG GTCAACTACG GCGTGACTGT GTTACCTACA TTTAAAGGCC
     1741 AGCCTAGCAA GCCGTTTGTG GGTGTTTTGT CGGCGGGCAT CAATGCGGCA TCACCGAATA
     1801 AGGAATTAGC GAAGGAATTC TTGGAGAATT ACCTTCTCAC GGACGAGGGC CTTGAGGCCG
     1861 TAAACAAGGA TAAGCCATTA GGCGCGGTGG CCTTAAAGAG CTATGAAGAG GAACTGGTCA
     1921 AGGACCCGCG CATCGCAGCT ACCATGGAGA ATGCTCAAAA GGGCGAGATT ATGCCCAATA
     1981 TTCCTCAAAT GTCGGCCTTT TGGTACGCGG TCCGCACCGC AGTAATTAAT GCAGCGTCCG
     2041 GACGCCAAAC CGTGGACGAG GCGCTTAAGG ATGCTCAAAC TAATTCGAGC TCGAACAACA
     2101 ACAACAATAA CAATAACAAC AACCTCGGGA TCGAGGGAAG GGGAATGAGA GACCTAGGCT
     2161 AGGTGGAGGC TCAGTGATGA TAAGTCTGCG ATGGTGGATG CATGTGTCAT GGTCATAGCT
     2221 GTTTCCTGTG TGAAATTGTT ATCCGCTCAG AGGGCACAAT CCTATTCCGC GCTATCCGAC
     2281 AATCTCCAAG ACATTAGGTG GAGTTCAGTT CGGCGTATGG CATATGTCGC TGGAAAGAAC
     2341 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     2401 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     2461 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     2521 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     2581 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     2641 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     2701 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     2761 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     2821 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     2881 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     2941 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     3001 ATCTTTTCTA CGGGGTCTGA CGCTCTATTC AACAAAGCCG CCGTCCCGTC AAGTCAGCGT
     3061 AAATGGGTAG GGGGCTTCAA ATCGTCCCCC CATACGATAT AAGTTGTTAC TAGTGCTTGG
     3121 ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG
     3181 TTCTGAGGTC ATTACTGG
//