LOCUS       pEPQD0CM0546_(MBP-TEV_C 3168 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(1718..1736)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(1753..1772)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            complement(2509..3168)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(1481..1501)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             287..1381
                     /label="MBP (maltose binding protein), A313"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     204..221
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1673..2261)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    287..1416
                     /label="N-term tag from pEPQD0CM0546"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    join(1429..3168,1..273)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1397..1416
                     /label="TEV cleavage site ENLYFQ/S"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC ATTGATACCT
      121 GAAACAAAAC CCATCGTACG GCCAAGGAAG TCTCCAATAA CTGTGATCCA CCACAAGCGC
      181 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TCATGCATAA TCCGCACGCA
      241 TCTGGAATAA GGAAGTGCCA TTCCGCCTGA CCTAGGTCTC TCCATGAAAA TCGAAGAAGG
      301 CAAATTAGTG ATATGGATCA ATGGAGATAA GGGATACAAT GGCTTAGCGG AAGTGGGAAA
      361 GAAGTTTGAA AAGGACACTG GGATCAAGGT TACGGTCGAA CACCCAGACA AGTTAGAGGA
      421 AAAGTTTCCG CAAGTAGCAG CCACAGGGGA CGGTCCGGAT ATCATTTTCT GGGCCCATGA
      481 TCGTTTCGGC GGATATGCCC AGAGTGGTCT TTTAGCGGAG ATTACTCCTG ATAAGGCATT
      541 TCAAGATAAA TTGTACCCTT TCACGTGGGA CGCGGTTCGC TATAATGGAA AACTTATCGC
      601 ATATCCTATT GCGGTAGAGG CACTGAGTCT CATCTACAAT AAGGACTTGC TCCCTAATCC
      661 GCCTAAGACA TGGGAAGAAA TTCCCGCCCT CGACAAGGAG CTCAAGGCAA AGGGCAAATC
      721 CGCCTTGATG TTTAATCTCC AGGAGCCATA TTTTACTTGG CCGTTGATCG CGGCCGATGG
      781 CGGCTACGCC TTTAAATACG AGAATGGTAA ATATGATATC AAGGATGTCG GAGTAGACAA
      841 TGCGGGTGCA AAGGCTGGAC TTACTTTCTT AGTGGATTTG ATCAAGAATA AGCATATGAA
      901 CGCTGATACG GACTATAGTA TTGCCGAGGC GGCGTTCAAC AAGGGTGAGA CTGCCATGAC
      961 TATTAATGGA CCTTGGGCTT GGTCGAATAT TGATACGAGT AAGGTCAACT ACGGCGTGAC
     1021 TGTGTTACCT ACATTTAAAG GCCAGCCTAG CAAGCCGTTT GTGGGTGTTT TGTCGGCGGG
     1081 CATCAATGCG GCATCACCGA ATAAGGAATT AGCGAAGGAA TTCTTGGAGA ATTACCTTCT
     1141 CACGGACGAG GGCCTTGAGG CCGTAAACAA GGATAAGCCA TTAGGCGCGG TGGCCTTAAA
     1201 GAGCTATGAA GAGGAACTGG CCAAGGACCC GCGCATCGCA GCTACCATGG AGAATGCTCA
     1261 AAAGGGCGAG ATTATGCCCA ATATTCCTCA AATGTCGGCC TTTTGGTACG CGGTCCGCAC
     1321 CGCAGTAATT AATGCAGCGT CCGGACGCCA AACCGTGGAC GAGGCGCTTA AGGATGCTCA
     1381 AACCAACTCT TCAAGCGAGA ACCTGTATTT TCAGTCAATG AGAGACCTAG GCTAGGTGGA
     1441 GGCTCAGTGA TGATAAGTCT GCGATGGTGG ATGCATGTGT CATGGTCATA GCTGTTTCCT
     1501 GTGTGAAATT GTTATCCGCT CAGAGGGCAC AATCCTATTC CGCGCTATCC GACAATCTCC
     1561 AAGACATTAG GTGGAGTTCA GTTCGGCGTA TGGCATATGT CGCTGGAAAG AACATGTGAG
     1621 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     1681 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     1741 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     1801 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     1861 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     1921 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     1981 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     2041 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     2101 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     2161 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     2221 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     2281 CTACGGGGTC TGACGCTCTA TTCAACAAAG CCGCCGTCCC GTCAAGTCAG CGTAAATGGG
     2341 TAGGGGGCTT CAAATCGTCC CCCCATACGA TATAAGTTGT TACTAGTGCT TGGATTCTCA
     2401 CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA AATCCAGATG GAGTTCTGAG
     2461 GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG ATATCAAATT ACGCCCCGCC
     2521 CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC
     2581 ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA
     2641 ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC
     2701 AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC
     2761 TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG
     2821 AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC
     2881 ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT
     2941 TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG
     3001 ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC
     3061 GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG
     3121 CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCAT
//