LOCUS       pEPQD0CM0548_(HiBiT-Trx 2427 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(739..758)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    50..373
                     /label="TrxA (Thioredoxin A)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1495..2154)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(704..722)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    415..2427
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2358..2375
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(659..1247)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    383..402
                     /label="TEV cleavage site ENLYFQ/S"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    14..46
                     /label="HiBiT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(467..487)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    14..402
                     /label="N-term tag from pEPQD0CM0548"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 AGGTCTCACC ATGGTAAGCG GCTGGCGTCT GTTCAAGAAA ATTAGCGGAA GTGATAAGAT
       61 TATTCATTTA ACAGATGACA GCTTTGATAC AGACGTGCTG AAGGCAGATG GAGCAATTCT
      121 GGTAGACTTT TGGGCGGAAT GGTGTGGCCC ATGTAAGATG ATTGCGCCGA TCTTAGACGA
      181 GATTGCAGAT GAGTACCAAG GTAAGTTGAC GGTGGCGAAG CTCAATATTG ACCAGAATCC
      241 GGGTACCGCA CCTAAGTACG GAATTCGCGG AATTCCAACC TTGTTACTCT TTAAGAATGG
      301 CGAGGTTGCC GCCACGAAGG TCGGCGCGTT AAGCAAGGGC CAACTGAAGG AATTCTTGGA
      361 TGCGAATTTA GCAGGCAGTA GCGAGAACCT GTATTTTCAG TCAATGAGAG ACCTAGGCTA
      421 GGTGGAGGCT CAGTGATGAT AAGTCTGCGA TGGTGGATGC ATGTGTCATG GTCATAGCTG
      481 TTTCCTGTGT GAAATTGTTA TCCGCTCAGA GGGCACAATC CTATTCCGCG CTATCCGACA
      541 ATCTCCAAGA CATTAGGTGG AGTTCAGTTC GGCGTATGGC ATATGTCGCT GGAAAGAACA
      601 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
      661 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
      721 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
      781 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
      841 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
      901 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
      961 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     1021 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     1081 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     1141 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     1201 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     1261 TCTTTTCTAC GGGGTCTGAC GCTCTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA
     1321 AATGGGTAGG GGGCTTCAAA TCGTCCCCCC ATACGATATA AGTTGTTACT AGTGCTTGGA
     1381 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT
     1441 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC
     1501 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA
     1561 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG
     1621 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT
     1681 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT
     1741 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT
     1801 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT
     1861 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC
     1921 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA
     1981 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG
     2041 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT
     2101 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC
     2161 TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC
     2221 ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG TCTCATTGAT ACCTGAAACA
     2281 AAACCCATCG TACGGCCAAG GAAGTCTCCA ATAACTGTGA TCCACCACAA GCGCCAGGGT
     2341 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTCATGC ATAATCCGCA CGCATCTGGA
     2401 ATAAGGAAGT GCCATTCCGC CTGACCT
//