LOCUS       pEPQD0CM0549_(SUMO-TEV_ 2355 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(587..1175)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            complement(1423..2082)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(667..686)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    14..301
                     /label="SUMO (Small ubiquitin-related modifier)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     2286..2303
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(632..650)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    14..330
                     /label="N-term tag from pEPQD0CM0549"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(395..415)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    311..330
                     /label="TEV cleavage site ENLYFQ/S"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    343..2355
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 AGGTCTCTCC ATGTCAGATA GTGAAGTGAA CCAAGAAGCA AAACCAGAGG TCAAGCCGGA
       61 AGTTAAACCG GAAACCCATA TTAACCTGAA AGTTAGCGAC GGTAGCTCGG AAATTTTCTT
      121 TAAAATTAAG AAAACTACGC CGCTGCGTCG CTTAATGGAA GCATTTGCCA AGCGTCAAGG
      181 CAAAGAGATG GATAGCCTGC GCTTTCTTTA TGATGGCATC CGCATCCAGG CGGACCAAAC
      241 TCCAGAGGAC TTAGATATGG AAGATAATGA CATCATCGAA GCCCATCGTG AGCAAATCGG
      301 CGGCATTGAA GAGAACCTGT ATTTTCAGTC AATGAGAGAC CTAGGCTAGG TGGAGGCTCA
      361 GTGATGATAA GTCTGCGATG GTGGATGCAT GTGTCATGGT CATAGCTGTT TCCTGTGTGA
      421 AATTGTTATC CGCTCAGAGG GCACAATCCT ATTCCGCGCT ATCCGACAAT CTCCAAGACA
      481 TTAGGTGGAG TTCAGTTCGG CGTATGGCAT ATGTCGCTGG AAAGAACATG TGAGCAAAAG
      541 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
      601 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
      661 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
      721 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
      781 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
      841 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
      901 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
      961 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     1021 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     1081 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     1141 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     1201 GGTCTGACGC TCTATTCAAC AAAGCCGCCG TCCCGTCAAG TCAGCGTAAA TGGGTAGGGG
     1261 GCTTCAAATC GTCCCCCCAT ACGATATAAG TTGTTACTAG TGCTTGGATT CTCACCAATA
     1321 AAAAACGCCC GGCGGCAACC GAGCGTTCTG AACAAATCCA GATGGAGTTC TGAGGTCATT
     1381 ACTGGATCTA TCAACAGGAG TCCAAGCGAG CTCGATATCA AATTACGCCC CGCCCTGCCA
     1441 CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC
     1501 GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT
     1561 GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT
     1621 GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG
     1681 GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG
     1741 CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA
     1801 AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT
     1861 ACGAAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA
     1921 CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG
     1981 GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG
     2041 GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC
     2101 TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA
     2161 GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTGATAC CTGAAACAAA ACCCATCGTA
     2221 CGGCCAAGGA AGTCTCCAAT AACTGTGATC CACCACAAGC GCCAGGGTTT TCCCAGTCAC
     2281 GACGTTGTAA AACGACGGCC AGTCATGCAT AATCCGCACG CATCTGGAAT AAGGAAGTGC
     2341 CATTCCGCCT GACCT
//