LOCUS       pEPQD0CM0551_(HiBiT-S-t 2346 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(658..677)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(578..1166)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    221..253
                     /label="HiBiT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            complement(1414..2073)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    257..298
                     /label="S-tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    334..2346
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2277..2294
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(623..641)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    221..321
                     /label="N-term tag from pEPQD0CM0551"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(386..406)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    302..319
                     /label="TEV cleavage site ENLYFQ/S"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 AGAAGACTAC TCACCATGAA AGAAACAGCA GCCGCGAAAT TCGAACGTCA GCATATGGAT
       61 TCAGAGAACC TGTATTTTCA GTCAATGCGA GTAGTCTTCA AATCCATATC TTCCAACGGA
      121 TTATGGAAAG AAGATCGCAG TTGCTTAAAA TGGCTCGACG AGCAACCATC GCGCTCAGTA
      181 CTGTTAAAGC TAGTTAATGA TGGTTCGCGG TCTCACCATG GTAAGCGGCT GGCGTCTGTT
      241 CAAGAAAATT AGCGGAAAGG AGACTGCCGC AGCTAAATTT GAGCGCCAAC ACATGGACAG
      301 CGAAAATTTA TACTTCCAAT CAATGAGAGA CCTAGGCTAG GTGGAGGCTC AGTGATGATA
      361 AGTCTGCGAT GGTGGATGCA TGTGTCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
      421 CCGCTCAGAG GGCACAATCC TATTCCGCGC TATCCGACAA TCTCCAAGAC ATTAGGTGGA
      481 GTTCAGTTCG GCGTATGGCA TATGTCGCTG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
      541 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
      601 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
      661 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
      721 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
      781 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
      841 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
      901 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
      961 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     1021 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     1081 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     1141 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     1201 CTCTATTCAA CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA ATGGGTAGGG GGCTTCAAAT
     1261 CGTCCCCCCA TACGATATAA GTTGTTACTA GTGCTTGGAT TCTCACCAAT AAAAAACGCC
     1321 CGGCGGCAAC CGAGCGTTCT GAACAAATCC AGATGGAGTT CTGAGGTCAT TACTGGATCT
     1381 ATCAACAGGA GTCCAAGCGA GCTCGATATC AAATTACGCC CCGCCCTGCC ACTCATCGCA
     1441 GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG
     1501 AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT
     1561 GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT
     1621 CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC
     1681 CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC
     1741 GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA
     1801 ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGAAATTC
     1861 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
     1921 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
     1981 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
     2041 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
     2101 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC
     2161 TCTTACGTGC CGATCAACGT CTCATTGATA CCTGAAACAA AACCCATCGT ACGGCCAAGG
     2221 AAGTCTCCAA TAACTGTGAT CCACCACAAG CGCCAGGGTT TTCCCAGTCA CGACGTTGTA
     2281 AAACGACGGC CAGTCATGCA TAATCCGCAC GCATCTGGAA TAAGGAAGTG CCATTCCGCC
     2341 TGACCT
//