LOCUS pEPMY1CB0001_(pOpinF_L1 6245 bp ds-DNA circular 16-OCT-2020 DEFINITION . KEYWORDS "marker:AmpR, polyhedrin 3' UTR" FEATURES Location/Qualifiers CDS 494..1171 /label="mRFP1" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 promoter 428..434 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(4028..4096) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 enhancer 5298..5601 /label="CMV enhancer" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(4501..5106) /label="ORF603" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 terminator 1216..1287 /label="rrnB T1 terminator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 122..231 /label="p10 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 442..458 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b protein_bind 2435..2468 /label="loxP" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b CDS complement(3236..4027) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 terminator 1661..1708 /label="T7 terminator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 404..409 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_recomb 4339..5166 /label="baculovirus recombination region (lef2/ORF603)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter 63..81 /label="T7 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 polyA_site 1518..1573 /label="β-globin poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac protein_bind 368..389 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter 5607..5884 /label="chicken β-actin promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 3'UTR 1809..2181 /label="polyhedrin 3' UTR" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin complement(2636..3223) /label="pUC origin of replication" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 RBS 476..487 /label="RBS" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 410..427 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 82..106 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b CDS complement(1838..2426) /label="ORF1629" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 terminator 1303..1330 /label="T7Te terminator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 4339..4463 /label="lef2" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 misc_recomb 1721..2426 /label="baculovirus recombination region (ORF1629)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 63..82 /label="QMD060_T7_prom" /note="sequence: taatacgactcactataggg" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer complement(2682..2701) /label="QMD132_LETprom2_F, 61.4" /note="sequence: gtttcgccacctctgacttg" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 81..100 /label="QMD162_pOpinF_lacO_outside_F 59.9" /note="sequence: GGGAATTGTGAGCGGATAAC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 2542..2564 /label="QMD542_211or_binding_seq_R" /note="sequence: TATCCACAGAATCAGGGGATAAC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(3363..3379) /label="QMD047 binding region (57.3)" /note="sequence: TCTCGCGGTATCATTGC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(2711..2734) /label="QMD131_LETprom1_F, 61.7, -3.89 self" /note="sequence: gggaaacgcctggtatctttatag" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(2540..2565) /label="211or (L0 FWD)" /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(1392..1411) /label="QMD163_pOpinF_outside_R 58.9" /note="sequence: CAGCCACCACCTTCTGATAG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 3180..3201 /label="QMD540_pBR322_seq_R" /note="sequence: ttgtttgcaagcagcagattac" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 3381..3394 /label="QMD048 binding region (60, 14bp)" /note="sequence: CCACGCTCACCGGC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(2680..2698) /label="QMD062_pJL1_outside(BR322)_F" /note="sequence: tcgccacctctgacttgag" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 ORIGIN 1 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGAA 61 ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCGG AGTTAATCCG 121 GGACCTTTAA TTCAACCCAA CACAATATAT TATAGTTAAA TAAGAATTAT TATCAAATCA 181 TTTGTATATT AATTAAAATA CTATACTGTA AATTACATTT TATTTACAAT CAAAGGAGAT 241 ATACCGAATT CCCATTGAGA CCGATATCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 301 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 361 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 421 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACATAC TAGAGAAAGA 481 GGAGAAATAC TAGATGGCTT CCTCCGAGGA CGTTATCAAA GAGTTCATGC GTTTCAAAGT 541 TCGTATGGAA GGTTCCGTTA ACGGTCACGA GTTCGAAATC GAAGGTGAAG GTGAAGGTCG 601 TCCGTACGAA GGTACCCAGA CCGCTAAACT GAAAGTTACC AAAGGTGGTC CGCTGCCGTT 661 CGCTTGGGAC ATCCTGTCCC CGCAGTTCCA GTACGGTTCC AAAGCTTACG TTAAACACCC 721 GGCTGACATC CCGGACTACC TGAAACTGTC CTTCCCGGAA GGTTTCAAAT GGGAACGTGT 781 TATGAACTTC GAGGACGGTG GTGTTGTTAC CGTTACCCAG GACTCCTCCC TGCAAGACGG 841 TGAGTTCATC TACAAAGTTA AACTGCGTGG TACCAACTTC CCGTCCGACG GTCCGGTTAT 901 GCAGAAAAAA ACCATGGGTT GGGAAGCTTC CACCGAACGT ATGTACCCGG AGGACGGTGC 961 TCTGAAAGGT GAAATCAAAA TGCGTCTGAA ACTGAAAGAC GGTGGTCACT ACGACGCTGA 1021 AGTTAAAACC ACCTACATGG CTAAAAAACC GGTTCAGCTG CCGGGTGCTT ACAAAACCGA 1081 CATCAAACTG GACATCACCT CCCACAACGA GGACTACACC ATCGTTGAAC AGTACGAACG 1141 TGCTGAAGGT CGTCACTCCA CCGGTGCTTA ATAACGCTGA TAGTGCTAGT GTAGATCGCT 1201 ACTAGAGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT 1261 ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG 1321 CCTTTCTGCG TTTATAGGTC TCAGCTTTCT AGGGATCCAC CAGTTTGTGA TTAACCTCAG 1381 GTGCAGGCTG CCTATCAGAA GGTGGTGGCT GGTGTGGCCA ATGCCCTGGC TCACAAATAC 1441 CACTGAGATC GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA 1501 GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT 1561 TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA 1621 GTATTTGGTT TAGAGTTTGG CAACATATGC CCATATGTAA CTAGCATAAC CCCTTGGGGC 1681 CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGCATGC GGAGGAAATT CTCCTTGAAG 1741 TTTCCCTGGT GTTCAAAGTA AAGGAGTTTG CACCAGACGC ACCTCTGTTC ACTGGTCCGG 1801 CGTATTAAAA CACGATACAT TGTTATTAGT ACATTTATTA AGCGCTAGAT TCTGTGCGTT 1861 GTTGATTTAC AGACAATTGT TGTACGTATT TTAATAATTC ATTAAATTTA TAATCTTTAG 1921 GGTGGTATGT TAGAGCGAAA ATCAAATGAT TTTCAGCGTC TTTATATCTG AATTTAAATA 1981 TTAAATCCTC AATAGATTTG TAAAATAGGT TTCGATTAGT TTCAAACAAG GGTTGTTTTT 2041 CCGAACCGAT GGCTGGACTA TCTAATGGAT TTTCGCTCAA CGCCACAAAA CTTGCCAAAT 2101 CTTGTAGCAG CAATCTAGCT TTGTCGATAT TCGTTTGTGT TTTGTTTTGT AATAAAGGTT 2161 CGACGTCGTT CAAAATATTA TGCGCTTTTG TATTTCTTTC ATCACTGTCG TTAGTGTACA 2221 ATTGACTCGA CGTAAACACG TTAAATAGAG CTTGGACATA TTTAACATCG GGCGTGTTAG 2281 CTTTATTAGG CCGATTATCG TCGTCGTCCC AACCCTCGTC GTTAGAAGTT GCTTCCGAAG 2341 ATGATTTTGC CATAGCCACA CGACGCCTAT TAATTGTGTC GGCTAACACG TCCGCGATCA 2401 AATTTGTAGT TGAGCTTTTT GGAATTGCGA TCGCATAACT TCGTATAGCA TACATTATAC 2461 GAAGTTATAA GCTCGGAACG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 2521 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 2581 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 2641 ATAGGCTCCG CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 2701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 2761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 2821 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 2881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 2941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 3001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 3061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 3121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 3181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGTTACC 3241 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 3301 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 3361 CTGCAATGAT ACCGCGAGAT CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 3421 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 3481 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 3541 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 3601 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 3661 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 3721 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 3781 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 3841 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 3901 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 3961 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 4021 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 4081 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 4141 GTCTCATGTC CGCGCGTTTC CTGCATCTTT TAATCAAATC CCAAGATGTG TATAAACGCG 4201 CCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG 4261 CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA 4321 AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA 4381 ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGAC 4441 TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGTCAAAT TTGTTTTTTA 4501 TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC 4561 GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG 4621 CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCCTCTT CTTCGTATTC 4681 CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT 4741 GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG 4801 GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG 4861 TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC 4921 GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT 4981 TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA 5041 CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC 5101 AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA 5161 GTTGCTGATG GCCGGCCGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA 5221 TCAATATATA GTTGCTCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC 5281 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC 5341 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG 5401 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 5461 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC 5521 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 5581 TTAGTCATCG CTATTACCAT GCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC 5641 CATCTCCCCC CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC 5701 AGCGATGGGG GCGGGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG 5761 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA 5821 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG 5881 GGCGGGAGTC GCTGCGCGCT GCCTTCGCCC CGTGCCCCGC TCCGCCGCCG CCTCGCGCCG 5941 CCCGCCCCGG CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC 6001 TCCTTCGGGC TGTAATTAGC GCTTGGTTTA ATGACGGCTT GTTTCTTTTC TGTGGCTGCG 6061 TGAAAGCCTT GAGGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGAGCGGCT CGGGGCTGTC 6121 CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC GGGGTTCGGC TTCTGGCGTG 6181 TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC 6241 TCCTG //