LOCUS       pEPMY1CB0001_(pOpinF_L1 6245 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
KEYWORDS    "marker:AmpR, polyhedrin 3' UTR"                                   
FEATURES             Location/Qualifiers
     CDS             494..1171
                     /label="mRFP1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     promoter        428..434
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(4028..4096)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     enhancer        5298..5601
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(4501..5106)
                     /label="ORF603"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     terminator      1216..1287
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        122..231
                     /label="p10 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    442..458
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    2435..2468
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             complement(3236..4027)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      1661..1708
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        404..409
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_recomb     4339..5166
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        63..81
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_site      1518..1573
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    368..389
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        5607..5884
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     3'UTR           1809..2181
                     /label="polyhedrin 3' UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(2636..3223)
                     /label="pUC origin of replication"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             476..487
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        410..427
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    82..106
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             complement(1838..2426)
                     /label="ORF1629"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     terminator      1303..1330
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4339..4463
                     /label="lef2"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_recomb     1721..2426
                     /label="baculovirus recombination region (ORF1629)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          63..82
                     /label="QMD060_T7_prom"
                     /note="sequence: taatacgactcactataggg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2682..2701)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          81..100
                     /label="QMD162_pOpinF_lacO_outside_F 59.9"
                     /note="sequence: GGGAATTGTGAGCGGATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2542..2564
                     /label="QMD542_211or_binding_seq_R"
                     /note="sequence: TATCCACAGAATCAGGGGATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(3363..3379)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(2711..2734)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2540..2565)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1392..1411)
                     /label="QMD163_pOpinF_outside_R 58.9"
                     /note="sequence: CAGCCACCACCTTCTGATAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3180..3201
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3381..3394
                     /label="QMD048 binding region (60, 14bp)"
                     /note="sequence: CCACGCTCACCGGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2680..2698)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGAA
       61 ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCGG AGTTAATCCG
      121 GGACCTTTAA TTCAACCCAA CACAATATAT TATAGTTAAA TAAGAATTAT TATCAAATCA
      181 TTTGTATATT AATTAAAATA CTATACTGTA AATTACATTT TATTTACAAT CAAAGGAGAT
      241 ATACCGAATT CCCATTGAGA CCGATATCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
      301 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
      361 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
      421 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACATAC TAGAGAAAGA
      481 GGAGAAATAC TAGATGGCTT CCTCCGAGGA CGTTATCAAA GAGTTCATGC GTTTCAAAGT
      541 TCGTATGGAA GGTTCCGTTA ACGGTCACGA GTTCGAAATC GAAGGTGAAG GTGAAGGTCG
      601 TCCGTACGAA GGTACCCAGA CCGCTAAACT GAAAGTTACC AAAGGTGGTC CGCTGCCGTT
      661 CGCTTGGGAC ATCCTGTCCC CGCAGTTCCA GTACGGTTCC AAAGCTTACG TTAAACACCC
      721 GGCTGACATC CCGGACTACC TGAAACTGTC CTTCCCGGAA GGTTTCAAAT GGGAACGTGT
      781 TATGAACTTC GAGGACGGTG GTGTTGTTAC CGTTACCCAG GACTCCTCCC TGCAAGACGG
      841 TGAGTTCATC TACAAAGTTA AACTGCGTGG TACCAACTTC CCGTCCGACG GTCCGGTTAT
      901 GCAGAAAAAA ACCATGGGTT GGGAAGCTTC CACCGAACGT ATGTACCCGG AGGACGGTGC
      961 TCTGAAAGGT GAAATCAAAA TGCGTCTGAA ACTGAAAGAC GGTGGTCACT ACGACGCTGA
     1021 AGTTAAAACC ACCTACATGG CTAAAAAACC GGTTCAGCTG CCGGGTGCTT ACAAAACCGA
     1081 CATCAAACTG GACATCACCT CCCACAACGA GGACTACACC ATCGTTGAAC AGTACGAACG
     1141 TGCTGAAGGT CGTCACTCCA CCGGTGCTTA ATAACGCTGA TAGTGCTAGT GTAGATCGCT
     1201 ACTAGAGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT
     1261 ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG
     1321 CCTTTCTGCG TTTATAGGTC TCAGCTTTCT AGGGATCCAC CAGTTTGTGA TTAACCTCAG
     1381 GTGCAGGCTG CCTATCAGAA GGTGGTGGCT GGTGTGGCCA ATGCCCTGGC TCACAAATAC
     1441 CACTGAGATC GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA
     1501 GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT
     1561 TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA
     1621 GTATTTGGTT TAGAGTTTGG CAACATATGC CCATATGTAA CTAGCATAAC CCCTTGGGGC
     1681 CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGCATGC GGAGGAAATT CTCCTTGAAG
     1741 TTTCCCTGGT GTTCAAAGTA AAGGAGTTTG CACCAGACGC ACCTCTGTTC ACTGGTCCGG
     1801 CGTATTAAAA CACGATACAT TGTTATTAGT ACATTTATTA AGCGCTAGAT TCTGTGCGTT
     1861 GTTGATTTAC AGACAATTGT TGTACGTATT TTAATAATTC ATTAAATTTA TAATCTTTAG
     1921 GGTGGTATGT TAGAGCGAAA ATCAAATGAT TTTCAGCGTC TTTATATCTG AATTTAAATA
     1981 TTAAATCCTC AATAGATTTG TAAAATAGGT TTCGATTAGT TTCAAACAAG GGTTGTTTTT
     2041 CCGAACCGAT GGCTGGACTA TCTAATGGAT TTTCGCTCAA CGCCACAAAA CTTGCCAAAT
     2101 CTTGTAGCAG CAATCTAGCT TTGTCGATAT TCGTTTGTGT TTTGTTTTGT AATAAAGGTT
     2161 CGACGTCGTT CAAAATATTA TGCGCTTTTG TATTTCTTTC ATCACTGTCG TTAGTGTACA
     2221 ATTGACTCGA CGTAAACACG TTAAATAGAG CTTGGACATA TTTAACATCG GGCGTGTTAG
     2281 CTTTATTAGG CCGATTATCG TCGTCGTCCC AACCCTCGTC GTTAGAAGTT GCTTCCGAAG
     2341 ATGATTTTGC CATAGCCACA CGACGCCTAT TAATTGTGTC GGCTAACACG TCCGCGATCA
     2401 AATTTGTAGT TGAGCTTTTT GGAATTGCGA TCGCATAACT TCGTATAGCA TACATTATAC
     2461 GAAGTTATAA GCTCGGAACG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     2521 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     2581 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     2641 ATAGGCTCCG CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     2701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     2761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     2821 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     2881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     2941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     3001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     3061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     3121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     3181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGTTACC
     3241 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
     3301 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
     3361 CTGCAATGAT ACCGCGAGAT CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
     3421 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
     3481 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
     3541 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
     3601 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
     3661 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
     3721 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
     3781 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
     3841 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
     3901 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
     3961 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
     4021 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
     4081 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
     4141 GTCTCATGTC CGCGCGTTTC CTGCATCTTT TAATCAAATC CCAAGATGTG TATAAACGCG
     4201 CCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG
     4261 CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA
     4321 AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA
     4381 ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGAC
     4441 TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGTCAAAT TTGTTTTTTA
     4501 TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC
     4561 GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG
     4621 CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCCTCTT CTTCGTATTC
     4681 CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT
     4741 GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG
     4801 GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG
     4861 TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC
     4921 GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT
     4981 TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA
     5041 CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC
     5101 AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA
     5161 GTTGCTGATG GCCGGCCGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA
     5221 TCAATATATA GTTGCTCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
     5281 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
     5341 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
     5401 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
     5461 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
     5521 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
     5581 TTAGTCATCG CTATTACCAT GCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC
     5641 CATCTCCCCC CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC
     5701 AGCGATGGGG GCGGGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG
     5761 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA
     5821 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG
     5881 GGCGGGAGTC GCTGCGCGCT GCCTTCGCCC CGTGCCCCGC TCCGCCGCCG CCTCGCGCCG
     5941 CCCGCCCCGG CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC
     6001 TCCTTCGGGC TGTAATTAGC GCTTGGTTTA ATGACGGCTT GTTTCTTTTC TGTGGCTGCG
     6061 TGAAAGCCTT GAGGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGAGCGGCT CGGGGCTGTC
     6121 CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC GGGGTTCGGC TTCTGGCGTG
     6181 TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC
     6241 TCCTG
//