LOCUS       Exported               13465 bp ds-DNA    circular SYN 13-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   JoshBlack099
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        225..423
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             441..621
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    668..793
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1286..1519
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2046..2163
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2208..2228
                     /label="M13-rev"
                     /note="M13-rev"
     primer_bind     2208..2224
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2245..2263
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2290..2382
                     /note="enhanced gRNA constant region"
     promoter        complement(4264..4512)
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    4361..4383
                     /label="U6 Seq Primer"
                     /note="U6 Seq Primer"
     promoter        complement(4527..4545)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4555..4571)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4677..4771
                     /note="enhgRNA cr2"
     misc_feature    4772..4790
                     /note="NEUROG3.2"
     promoter        4792..5116
                     /note="mU6"
     promoter        complement(5117)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5127..6281
                     /note="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             6310..7026
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7036..7092
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7093..7689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     protein_bind    7705..7738
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7763..8351
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8234..8245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(8354..8370)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8876..9056
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9088..9312
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9358..9786
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9800..10129
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      9980..10115
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        10177..10224
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             10243..10617
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10747..10868
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10917..10933)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10941..10957
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10965..10995)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11010..11031
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11319..11907)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12078..12938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12939..13043)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        13309..223
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
       61 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      121 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      181 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      241 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      301 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      361 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      421 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
      781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
      841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
      901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
      961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1921 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     1981 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2041 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2101 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2161 tttcgggttt attacaggga cagcagagat ccagtttggt taatacacag gaaacagcta
     2221 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     2281 ccaccgcgga aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
     2341 ttatttaaac ttgctatgct gtttccagca tagctcttaa acagagacgt acaaaaaaga
     2401 gcaagaagct aaaaaagatt taaaaattat ttttagcgca gttaatggaa caggaactaa
     2461 atttacccca aaaatattac gtgaatcagg atataacgtt attgaggttg aagagcatgc
     2521 atttgaagat gaaacattta aaaatgttgt aaatccaaat ccagaatttg atcctgcatg
     2581 aaaaataccg cttgaatatg gtattaaaca tgatgcagat attattatta tgaatgaccc
     2641 agatgctgac agatttggaa tggcaataaa acatgatggt cattttgtaa gattagatgg
     2701 aaatcaaaca ggaccaattt taattgattg aaaattatca aatctaaaac gcttaaatag
     2761 cattccaaaa aatccggctc tatattcaag ttttgtaaca agtgatttgg gtgatagaat
     2821 cgctcatgaa aaatatggag ttaatattgt aaaaacttta actggattta aatgaatggg
     2881 tagagaaatt gctaaagaag aagataacgg attaaatttt gtttttgctt atgaagaaag
     2941 ttatggatat gtaattgatg actcagctag agataaagat ggaatacaag cttctatatt
     3001 aatagcagag gctgcttgat tttataaaaa acaaaataaa acattagtag actatttaga
     3061 agatttattt aaagaaatgg gtgcatatta cactttcact ttaaacttga attttaaacc
     3121 agaagaaaag aaattaaaaa ttgaaccatt aatgaaatca ttgagagcaa cacccttaac
     3181 tcaaattgct ggacttaaag ttgttaatgt tgaagactac atcgatggaa tgtataatat
     3241 gccaggacaa gacttactaa aattttattt agaagataag tcatgatttg ctgttcgccc
     3301 aagtggaact gaacctaaac taaaaattta ttttataggt gttggtgaat ctgttcaaaa
     3361 cgctaaagtt aaagtagacg aaattattaa agaattaaaa ttaaaaatga atatatagga
     3421 gaaaaaatga aactaaacaa atatatagat cacacattat taaaacaaga tgctacgaaa
     3481 gctgaaatta aacaattatg tgatgaagca attgaatttg attttgcaac agtttgtgtt
     3541 aattcatatt gaacaagcta ttgtaaagaa ttattaaaag gcacaaatgt aggaataaca
     3601 aatgttgtag gttttcctct aggtgcatgc acaacagcta caaaagcatt cgaagtttct
     3661 gaagcaatta aagatggtgc aacagaaatt gatatggtat taaatattgg tgcattaaaa
     3721 gacaaaaatt atgaattagt tttagaagac atgaaagctg taaaaaaagc agctggatca
     3781 catgttgtta aatgtattat ggaaaattgt ttattaacaa aagaagaaat catgaaagct
     3841 tgtgaaatag ctgttgaagc tggattagaa tttgttaaaa catcaacagg attttcaaaa
     3901 tcaggtgcaa catttgaaga tgttaaacta atgaagtcag ttgttaaaga caatgcttta
     3961 gttaaagcag ctggtggagt tagaacattt gaagatgctc aaaaaatgat tgaagcagga
     4021 gctgaccgct taggaacaag tggtggagta gctattatta aaggtgaaga aaacaacgcg
     4081 agttactaaa actagcgttt ttttattttg ctcattttta ttaaaagttt gcaaaaagga
     4141 acataaaaat tctaattatt gatactaaag ttattaaaaa gaagattttg gttgatttta
     4201 taaaggtcat agaatataat attttagcat gtgtattttg tgtgctcatt tacaaccgtc
     4261 tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
     4321 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
     4381 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
     4441 aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
     4501 aaataggccc tcggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4561 cgtcgtttta caacattaac ccgtgtcggc tccagatctg gcctccgcgc cgggttttgg
     4621 cgcctcccgc gggcgccccc ctcctcacgg cgagctgaat ctgggcgact cagtacaaaa
     4681 aaagcagccg actcggctgc cactttttca agttgtgtac ggactagcct tatttgaact
     4741 tgctatgcag ctttctgctt agctctcaaa cctctgtttg ctctctcgag caaacaaggc
     4801 ttttctccaa gggatattta tagtctcaaa acacacaatt actttacagt tagggtgagt
     4861 ttccttttgt gctgtttttt aaaataataa tttagtattt gtatctctta tagaaatcca
     4921 agcctatcat gtaaaatgta gctagtatta aaaagaacag attatctgtc ttttatcgca
     4981 cattaagcct ctatagttac taggaaatat tatatgcaaa ttaaccgggg caggggagta
     5041 gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc gggcctagag atggcggcgt
     5101 cggatctatc tgagaccggg ctaacggctg ccacgtcaga cgaagggcgc agcgagcgtc
     5161 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     5221 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     5281 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     5341 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     5401 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     5461 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     5521 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     5581 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     5641 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     5701 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     5761 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     5821 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     5881 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     5941 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     6001 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     6061 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     6121 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     6181 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     6241 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     6301 gactctagaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6361 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6421 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6481 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6541 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6601 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6661 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6721 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     6781 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6841 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6901 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6961 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     7021 tacaagggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     7081 aatcctggac cgatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     7141 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     7201 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     7261 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     7321 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     7381 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7441 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tgtcgcccga ccaccagggc
     7501 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     7561 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7621 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7681 cccggtgcct aaagttgata atagataact tcgtatagca tacattatac gaagttatga
     7741 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     7801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     8161 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8341 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     8401 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8461 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8521 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8581 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     8641 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     8701 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     8761 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     8821 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     8881 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8941 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9001 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     9061 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     9121 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     9181 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     9241 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     9301 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     9361 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9421 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9481 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9541 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9601 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9661 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9721 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9781 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9961 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10021 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
    10081 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
    10141 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
    10201 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
    10261 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
    10321 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
    10381 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
    10441 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
    10501 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
    10561 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
    10621 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    10681 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    10741 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10801 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10861 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
    10921 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    10981 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    11041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    11101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    11161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11221 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    11281 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11341 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11401 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11461 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11521 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11581 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11641 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    11701 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    11761 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11821 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    11881 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11941 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    12001 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    12061 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    12121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    12181 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    12241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    12301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    12361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    12421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    12481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    12541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    12601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    12661 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    12721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    12781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    12841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    12901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    12961 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    13021 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    13081 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    13141 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    13201 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
    13261 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
    13321 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    13381 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    13441 cattgacgtc aataatgacg tatgt
//
